Acute Contact Toxicity of Six Pesticides in Honeybees (Apis Mellifera Meda) in Iran

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Pollination has an important role in both agricultural production and wild plant reproduction. For the pollination of crops, agriculture relies largely on managed colonies of the honeybee Apis mellifera. Worker bees are primarily affected by pesticides. The symptoms of poisoning vary depending on the developmental stage of the individual bee and kind of chemical employed. The acute contact toxicity of insecticides (phosalone and pirimicarb), acaricide (propargite), insecticide and acaricide (fenpropathrin), fungicides and bactericides (copper oxychloride and bordeaux mixture) was assessed in Iran through laboratory experiments. The median lethal concentrations (LC50-24h, LC50-48h and LC50-72h) were evaluated for the purposes of this research. Results showed that fenpropathrin had high toxicity; LC50-24h, LC50-48h and LC50-72h were 5.7, 3.2 and 2.9 ppm respectively. Additionally, the bordeaux mixture had the minimum contact toxicity on honeybees with LC50-24h, LC50-48h and LC50-72h being 79,926; 69,552 and 69,045 ppm respectively and was safe and non-toxic in honeybees.

Adams, R.G., & Bartholomew, C. (2012). Protecting honey bees from pesticide poisoning. University of Connecticut, 7 pp.

EFSA. (European Food Safety Authority). (2013). EFSA Guidance Document on the risk assessment of plant protection products on bees (Apis mellifera, Bombus spp. and solitary bees). EFSA Journal, 11(7), 268pp.

EPPO/Council of Europe. (1993). Decision-Making Scheme for the Environmental Risk Assessment of Plant Protection Products - Honeybees. EPPO bulletin, 23(1), 151-165.

Gallai, N., Salles, J.M., Settele, J., & Vaissière, B.E. (2009). Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Journal of Ecological Economics, 68(3), 810-821.

Gauthier, M., Dacher M., Thany, S.H., Niggebrugge, C., Deglise, P., Kljucevic, P., Armengaud, C., Grünewald, B. (2010). Involvement of alpha-bungarotoxin-sensitive nicotinic receptors in long-term memory formation in the honeybee (Apis mellifera). Neurobiology of Learning and Memory, 86(1), 164-174.

Hepburn, R., & Radloff, S. (2011). Honeybees of Asia. Springer-Verlag Berlin Heidelberg.

Kevan, P. G. (1999). Pollinators as bioindicators of the state of the environment: species, activity and diversity. Agriculture Ecosystems and Environment, 74, 373-393.

Laurino, D., Manino, A., Patetta, A., Ansaldi, M., Porporato, M. (2010). Acute oral toxicity of neonicotinoids on different bee strains. Redia, 93, 99-102.

Laurino, D., Manino, A., Patetta, A., Ansaldi, M., Porporato, M. (2011), Toxicity of neonicotinoid insecticides to honey bees: laboratory tests. Bulletin of Insectology, 64, 107-113.

Laurino, D., Manino, A., Patetta, A., Ansaldi, M., Porporato, M. (2013). Toxicity of neonicotinoid insecticides on different honey bee genotypes. Bulletin of Insectology, 66(1), 119-126.

Mayer, D.F., Johansen, C.A., & Baird, C.R. (1999). How to reduce bee poisoning from pesticides. Pacific Northwest Extension Publication PNW 518.

Mullin, C.A., Frazier, M., Frazier, J.L., Ashcraft, S., Simonds, R., van Engelsdorp, D., Pettis, J.S. (2010). High levels of miticides and agrochemicals in North American apiaries. implications for honey bee health. PLoS ONE 5:3 pp. 9754.

OECD. (1998). Gaudiness for the testing of chemical No. 213. Honeybees, Acute Oral Toxicity Test. Section 2. effect on biotic systems. OECD, Paris, DOI: 10.178719789264070165-en.

OEPP/EPPO. (2010a). EPPO Standards PP1/170 (4). Efficacy evaluation of plant protection products, Side-effects on honeybees. OEPP/EPPO Bulletin, 40, 313-319.

OEPP/EPPO. (2010b). EPPO Standards PP3/10 (3). Environmental risk assessment for plant protection products. Chapter 10: honeybees. OEPP/EPPO Bulletin, 40, 323- 331.

Piechowicz, B., Grodzicki, P., Stawarczyk, M., Stawarczyk, K. (2013). Circadian and seasonal changes in honeybee (Apis mellifera) worker susceptibility to diazinon, teflubenzuron, pirimicarb, and indoxacarb. The Polish Journal of Environmental Studies, 22(5), 1457-1463.

Rasuli, F., Nazemi Rafie, J., & Sadeghi, A. (2015). The acute oral toxicity of commonly used pesticides in Iran, to honeybees (Apis mellifera meda). Journal of Apicultural Science, 59(1), 17-26. DOI 10.1515/jas-2015-0007

Robertson, J. L., & Preisler, H. K. (1992). Pesticide Bioassays with Arthropods. CRC Press Boca Raton, FL, USA.

Sanford, M.T. (2009). Protecting honey bees from pesticides. University of Florida 13 pp.

Srinivasan, M. V. (2011). Honeybees as a Model for the Study of Visually Guided Flight, Navigation, and Biologically Inspired Robotics. Physiological Reviews, 91, 413-460.

Tesoriero, D., Maccagnani, B., Santi, F., & Celli, G. (2003). Toxicity of three pesticides on larval instars of Osmia cornuta: preliminary results. Bulletin of Insectology, 56(1), 169-171.

Vandame, R., & Belzunces, L.P. (1998). Joint actions of deltamethrin and azole fungicides on honey bee thermoregulation. Neuroscience Letters, 25(1), 57-60.

VanEngelsdorp, D., Hayes, J., Underwood, R., & Pettis, J. (2009). A survey of honey bee colony losses in the United States, fall 2008 to spring 2009. Journal of Apicultural Research, 49(1), 7-14.

Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

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