Methoprene and Temperature Stimulation of Emergence and Longevity in Adults of Osmia Rufa L. (Megachilidae; Apoidea) During Wintering Period

Open access

Abstract

In this study methoprene, a juvenile hormone analogues, was tested as a factor that stimulates the end of diapause, bee activation and emergence. In addition, the survival of bees was checked when combined with an application of methoprene. The experimental activation of wintering bees was carried out once a month between December and March, Three groups of bee cocoons were selected for each activation term: treated with either methoprene or acetone as vehicle control and untreated as controls. After the applications were finished the cocoons were incubated at temperature 26°C. The emerged adult males and females were then kept in the laboratory and fed sucrose solution to evaluate the longevity of bees. The application of juvenile hormone analogue reduced the emergence time of adult bees in December, February and March. The rate of emergence presented in the form of cumulative percentage of emerged adult bees indicated that the bees treated with methoprene started to emerge 1-2 days earlier than bees from acetone and control groups and finished emergence 2-5 days earlier too. Methopren application did not reduce the longevity of the bees after emergence. Moreover, the median longevity of these females was higher than untreated in December and February.

Bosch J., & Blas, J. (1994). Effect of over-wintering and incubation temperatures on adult emergence in Osmia cornuta Latr. Apidologie, 25, 265-277.

Bosch, J., & Kemp, W.P. (2000). Development and emergence of the orchard pollinator Osmia lignaria (Hymenoptera: Megachilidae). Environmental Entomology, 29, 8-13. DOI: 10.1603/0046-225X-29.1.8

Bosch, J., & Kemp, W.P. (2003). Effect of wintering duration and temperature on survival and emergence time in males of the orchard pollinator Osmia lignaria (Hymenoptera: Megachilidae). Environmental Entomology, 32, 711-716. DOI: 10.1603/0046-225X-32.4.711

Bosch, J., & Kemp, W.P (2004). Effect of pre-wintering and wintering temperature regimes on weight loss, survival, and emergence time in the mason bee Osmia cornuta (Hymenoptera: Megachilidae). Apidologie, 35, 469-479. DOI: 10.1051/apido:2004035

Bosch, J., Kemp, W.P., & Peterson, S.S. (2000). Management of Osmia lignaria (Hymenoptera: Megachilidae) populations for almond pollination: methods to advance bee emergence. Environmental Entomology, 29, 874-883. http://dx.doi.org/10.1603/0046-225X-29.5.874

Bosch, J., Sgolastra, F., & Kemp, W.P. (2010). Timing of eclosion affects diapause development, fat body consumption and longevity in Osmia lignaria, a univoltine, adult-wintering solitary bee. Journal of Insect Physiology, 56, 1949-1957. DOI:10.1016/j.jinsphys.2010.08.017

De Loof, A. (2008). Ecdysteroids, juvenile hormone and insect neuropeptides: recent successes and remaining major challenges. General and Comparative Endocrinology, 155, 3-13. http://dx.doi.org/10.1016/j.ygcen.2007.07.001

Denlinger, D.L. (2002). Regulation of diapause. Annual Review of Entomology, 47, 93-122. DOI: 10.1146/annurev.ento.47.091201.145137

Fliszkiewicz M., Giejdasz, K., & Wilkaniec, Z. (2011). The effect of diapause termination time on the successful insemination in Osmia rufa L. Journal of Apicultural Science, 55, 73:78.

Hondelmann, P., & Poehling, H.M. (2007). Diapause and overwintering of the hoverfly Episyrphus balteatus. Entomologia Experimentalis et Applicata, 124, 189-200. DOI: 10.1111 /j.1570-7458.2007.00568.x

Kaminski P. (2010). Gametoclonal and Somaclonal Variation among Head Cabbage Androgenic Lines of R1 and R2 Generations Obtained from Jaguar F1 Hybrid. Journal of Agricultural Science, 2(2), 119-128.

Klowden, M. J., & Chambers, G.M. (1989). Ovarian development and adult mortality in Aedes aegypti treated with sucrose, juvenile hormone and methoprene. Journal of Insect Physiology, 35, 513-517. https://doi.org/10.1016/0022-1910(89)90058-9

Koštál, V. (2006). Eco-physiological phases of insect diapause. Journal of Insect Physiology, 52, 113-127. http://dx.doi.org/10.1016/j.jinsphys.2005.09.008

Malka, O., Katzav-Gozansky, T., & Hefetz, A. (2009). Uncoupling fertility from fertility-associated pheromones In worker honeybees (Apis melifera). Journal of Insect Physiology, 55, 205-209. http://dx.doi.org/10.1016/j.jinsphys.2008.11.002

Mitchel, C.J., & Briegel, H. (1989). Inability of diapausing Culex pipiens (Diptera: Culicidae) to use blood for producing lipid reserves for overwinter survival. Journal of Medical Entomology, 26, 318-326.

Robinson, G.E., Strambi, C., Strambi, A., & Feldlaufer, M.F. (1992). Reproduction in worker honey bees is associated with low juvenile hormone titers and rates of biosynthesis. General and Comparative Endocrinology, 87, 471-480.

Rust R., Torchio P., & Trostle G. (1989). Late embryogenesis and immature development of Osmia rufa cornigera (Rossi) (Hymenoptera: Megachilidae), Apidologie, 20, 359-367.

Schittenhelm, S., Giadis, T., & Rao, V.R. (1997). Efficiency of various insects in germplasm regeneration of carrot, onion and turnip rape accessions. Plant Breeding, 116, 369-375.

Sedivy, C., & Dorn, S. (2014). Towards a sustainable management of bees of the subgenus Osmia (Megachilidae; Osmia) as fruit tree pollinators. Apidologie, 45, 88-105. DOI: 10.1007/ s13592-013-0231-8

Sgolastra, F., Bosch, J., Molowny-Horas, R., Maini, S., & Kemp, W.P. (2010). Effect of temperature regime on diapause intensity in and adult - wintering Hymenopteran with obligate diapause. Journal of Insect Physiology, 56, 185-194. DOI: 10.1016/j.jinsphys.2009.10.001

Simonet, G., Poels, J., Claeys, I., Van Loy, T., Franssens, V., De Loof, A., & Vanden Broeck, J. (2004). Neuroendocrinological and molecular aspects of insect reproduction. Journal of Neuroendocrinology, 16, 649-659.

Strachecka A., Chobotow J., Paleolog J., Łoś A., Schulz M., Teper D., Kucharczyk H., & Grzybek M. (2017) Insights into the biochemical defenceand methylation of the solitary bee Osmia rufa L: A foundation for examining eusociality development.” PLoS ONE 12 (4): e0176539.

Tasei J. N. (1973). Le comportement de nidification chez. lOsmia: Osmia cornuta Latr. et Osmia rufa L (Hymenoptera: Megachilidae). Apidologie, 4, 195-315.

Wasielewski, O., Giejdasz, K., Wojciechowicz, T., & Skrzypski, M. (2011a). Ovary growth and protein levels in ovary and fat body during adult-wintering period in the red mason bee, Osmia rufa L. Apidologie, 42, 749-758. DOI: 10.1007/s13592-011-0084-y

Wasielewski, O., Wojciechowicz, T., Giejdasz, K., & N. Krishnan N. (2011b). Influence of methoprene and temperature on diapause termination in adult females of the over-wintering solitary bee, Osmia rufa L. Journal of Insect Physiology, 57, 1682-1688. DOI:10.1016/j.jinsphys.2011.09.002

Wilkaniec, Z., & Radajewska, B. (1996). Solitary bee Osmia rufa L.(Apoidea, Megachilidae) as pollinator of strawberry cultivated in an unheated plastic tunnel. In Proceedings of III International Strawberry Symposium (p. 489-494).

Wilkaniec, Z., & Maciejewska, M. (1998). Wykorzystanie pszczoły murarki ogrodowej (Osmia rufa L., Megachilidae) do zapylania brzoskwini w uprawie szklarniowej. Zeszyty Naukowe Akademii Rolniczej w Krakowie, 57, 869-873. [in Polish]

Wójtowski, F., & Wilkaniec, Z. (1978). Rearing and utilization of solitary bees settled in nest traps (in Polish). Instrukcja Wdrożeniowa AR Poznań 1-10.

Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

Journal Information


IMPACT FACTOR 2017: 0.75
5-year IMPACT FACTOR: 1.007

CiteScore 2017: 0.92

SCImago Journal Rank (SJR) 2017: 0.345
Source Normalized Impact per Paper (SNIP) 2017: 0.461

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 163 163 13
PDF Downloads 89 89 8