Toxicity of Spirotetramat on Solitary Bee Larvae, Osmia Cornuta (Hymenoptera: Megachilidae), in Laboratory Conditions

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Abstract

In this paper we assessed, under laboratory conditions, the toxicity of an active substance on solitary bee larvae of Osmia cornuta (Hymenoptera: Megachilidae). A field-realistic dose of the systemic insecticide spirotetramat was applied to the mass provisions. The insecticide’s effects on several life-cycle parameters were studied in males and females. Our results showed a significantly shorter post-emergence longevity in bees exposed to spirotetramat during the larval stage, compared to the control. The observed reduction in longevity was 18 and 15%, respectively, in males and females. Mortality rate and other biological traits (larval and spinning duration, emergence time, food/body conversion rate) did not show significant differences between the two treatments. The method described in this study can be used to test the effects of toxic substances (i.e. agrochemicals) on bees. Moreover, it can constitute a basis for the development of a standardised protocol in the first tier of the Environmental Risk Assessment for solitary bees.

Abbott V. A., Nadeau J. L., Higo H. A., Winston M. L. (2008) Lethal and sublethal effects of imidacloprid on Osmia lignaria and clothianidin on Megachile rotundata (Hymenoptera: megachilidae). Journal of Economic Entomology 101: 784-796.

Arena M., Sgolastra F. (2014) A meta-analysis comparing the sensitivity of bees to pesticides. Ecotoxicology 23: 324-334.

Babendreier D., Kalberer N., Romeis J., Fluri P., Bigler F. (2004) Pollen consumption in honey bee larvae: a step forward in the risk assessment of transgenic plants. Apidologie 35: 293-300.

Bosch J., Kemp W. P. (2000) Development and emergence of the orchard pollinator, Osmia lignaria (Hymenoptera, Megachilidae). Environmental Entomology 29: 8-13.

Bosch J., Kemp W. P. (2001) How to manage the blue orchard bee as an orchard pollinator. Sustainable Agricultural Network. Beltsville, Maryland. 98 pp.

Bosch J., Kemp W. P. (2002) Developing and establishing bee species as crop pollinators: the example of Osmia spp. (Hymenoptera: Megachilidae) and fruit trees. Bulletin of Entomological Research 92: 3-16.

Bosch J., Kemp W. P. (2004) Effect of pre-wintering and wintering temperature regimes on weight loss, survival, and emergence time in the mason bee Osmia cornuta (Hymenptera: Megachilidae). Apidologie 35: 469-479.

Bosch J., Vicens N. (2002) Body size as an estimator of production costs in a solitary bee. Ecological Entomology 27: 129-137.

Bosch J., Vicens N. (2005) Sex allocation in the solitary bee Osmia cornuta: do females behave in agreement with Fisher’s theory? Behavioral Ecology and Sociobiology 59: 124-132.

Bosch J., Vicens N. (2006) Relationship between body size, provisioning rate, longevity and reproductive success in females of the solitary bee Osmia cornuta. Behavioral Ecology and Sociobiology 60: 26-33.

Bosch J., Sgolastra F., Kemp W. P. (2008) Life Cycle Ecophysiology of Osmia Mason Bees Used as Crop Pollinators. In: James R. R., Pitts-Singer T. L. (Eds.) Bee Pollination in Agricultural Ecosystems. Oxford University Press. Oxford, UK: 83-104.

Bosch J., Sgolastra F., Kemp W. P. (2010) Timing of eclosion affects diapause development, fat body consumption and longevity in Osmia lignaria, a univoltine, adultwintering solitary bee. Journal of Insect Physiology 56: 1949-1957.

Brittain C., Potts S. G. (2011) The potential impacts of insecticides on the life-history traits of bees and the consequences for pollination. Basic and Applied Ecology 12: 321-331.

Crailsheim K., Brodschneider R., Aupinel P., Behrens D., Genersch E., Vollmann J., Riessberger-Gallé U. (2012) Standard methods for artificial rearing of Apis mellifera larvae. Journal of Apicultural Research 52: 1-15.

Dmochowska K., Giejdasz K., Fliszkiewicz M., Żółtowska K. (2012) Changes in antioxidative system of red mason bee (Osmia rufa) (Hymenoptera: Megachilidae) induced by artificially elongated postdiapause. Journal of Apicultural Science 56: 99-106.

EFSA (2013) EFSA Guidance Document on the risk assessment of plant protection products on bees (Apis mellifera, Bombus spp. and solitary bees). EFSA Journal 11(7): 3295. 266 pp.

European Commissiom (2009) Regulation EC No 1107/2009 of the European Parliament and of the Council. Official Journal of European Union 309: 1-50.

Gallai N., Salles J. M., Settele J., Vaissiere B. E. (2009) Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Ecological Economics 68: 810-821.

Garibaldi L. A., Steffan-Dewenter I., Winfree R., Aizen M. A., Bommarco R., Cunningham S. A., Kremen C., Carvalheiro L. G., Harder L. D., Afik O., Bartomeus I., Benjamin F., Boreux V., Cariveau D., Chacoff N. P., Dudenhoffer J. H., Freitas B. M., Ghazoul J., Greenleaf S., Hipolito J., Holzschuh A., Howlett B., Isaacs R., Javorek S. K., Kennedy C. M., Krewenka K. M., Krishnan S., Mandelik Y., Mayfield M. M., Motzke I., Munyuli T., Nault B. A., Otieno M., Petersen J., Pisanty G., Potts S. G., Rader R., Ricketts T. H., Rundlof M., Seymour C. L., Schuepp C., Szentgyorgyi H., Taki H., Tscharntke T., Vergara C. H., Viana B. F., Wanger T. C., Westphal C., Williams N., Klein A. M. (2013) Wild pollinators enhance fruit set of crops regardless of honey bee abundance. Science 339: 1608-1611.

Gradish A. E., Scott-Dupree C. D., Cutler G. C. (2012). Susceptibility of Megachile rotundata to insecticides used in wild blueberry production in Atlantic Canada. Journal of Pest Science 85: 133-140.

Hodgson E. W., Pitts-Singer T. L., Barbour J. D. (2011) Effects of the insect growth regulator, novaluron on immature alfalfa leafcutting bees, Megachile rotundata. Journal of Insect Science 11: 43. Available at: http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1050&context=ent_pubs

Huntzinger C. I., James R. R., Bosch J., Kemp W. P. (2008) Laboratory biossays to evaluate fungicides for chalkbrood control in larvae of the alfalfa leafcutting bee (Hymenoptera; Megachilidae). Journal of Economic Entomology 101: 660-667.

Klein A. M., Vaissiere B. E., Cane J. H., Steffan-Dewenter I., Cunningham S. A., Kremen C., Tscharntke T. (2007) Importance of pollinators in changing landscapes for world crops. Proceedings of the Royal Society B 274: 303-313.

Konrad R., Ferry N., Gatehouse A. M. R., Babendreier D. (2008) Potential effects of oilseed rape expressing oryzacystatin-1 (OC-1) and of purified insecticidal proteins on larvae of the solitary bee Osmia bicornis. PLoS ONE 3(7): e2664.

Ladurner E., Bosch J., Kemp W. P., Maini S. (2005) Assessing delayed and acute toxicity of five formulated fungicides to Osmia lignaria Say and Apis mellifera. Apidologie 36: 449-460.

Ladurner E., Bosch J., Kemp W. P., Maini S. (2008) Foraging and Nesting Behavior of Osmia lignaria (Hymenoptera: Megachilidae) in the Presence of Fungicides: Cage Studies. Journal of Economic Entomology 101: 647-653.

Maus C. (2008) Ecotoxicological profile of the insecticide spirotetramat. Bayer Crop Science Journal 61: 159-180.

Peach M. L., Alston D. G., Tepedino V. J. (1995) Sublethal effects of carbaryl bran bait on nesting performance, parental investment, and offspring size and sex ratio of the alfalfa leafcutting bee (Hymenoptera: Megachilidae). Environmental Entomology 24: 34-39.

Peters D. S. (1977) Systematik und Zoogeographie der westpaleärktischen Arten von Osmia’s str., Monosmia, und Orientosmia. Senckenbergiana Biologica 58: 287-346.

Radmacher S., Strohm E. (2010) Factors affecting offspring body size in the solitary bee Osmia bicornis (Hymenoptera, Megachilidae). Apidologie 41: 169-177.

Radmacher S., Strohm E. (2011) Effects of constant and fluctuating temperatures on the development of the solitary bee Osmia bicornis (Hymenoptera: Megachilidae). Apidologie 42: 711-720.

Rogers D., Williams G., Lam C., Fischer D., Hackenberg D. (2010) Movento®, Citrus and Honey Bees. Results of a successful cooperative study. In: Proceedings of the American Bee Research Conference. Orlando-USA. 14-15 January 2010. American Bee Journal: 506.

Rundlöf M., Andersson G. K. S., Bommarco R., Fries I., Hederström V., Herbertsson L., Jonsson O., Klatt B. K., Pedersen T. R., Yourstone J., Smith H. G. (2015) Seed coating with a neonicotinoid insecticide negatively affects wild bees. Nature 521(7550): 77-80.

Sandrock C., Tanadini L. G., Pettis J. S., Biesmeijer J. C., Potts S. G., Neumann P. (2014) Sublethal neonicotinoid insecticide exposure reduces solitary bee reproductive success. Agricultural and Forest Entomology 16: 119-128.

Schindler M., Diestelhorst O., Härtel S., Saure C., Schanowski A., Schwenninger H. R. (2013) Monitoring agricultural ecosystems by using wild bees as environmental indicators. Biorisk 8: 39-51.

Sgolastra F., Kemp W. P., Maini S., Bosch J. (2012) Duration of prepupal summer dormancy regulates synchronization of adult diapause with winter temperatures in bees of the genus Osmia. Journal of Insect Physiology 58: 924-933.

Sgolastra F., Kemp W. P., Buckner J. S., Pitts-Singer T. L., Maini S., Bosch J. (2011) The long summer: Pre-wintering temperatures affect metabolic expenditure and winter survival in a solitary bee. Journal of Insect Physiology 57: 1651-1659. Statistica ver. 7. (2004) StatSoft Inc.

Tasei J. N., Carre S., Moscatelli B., Grondeau C. (1988) Recherche de la D.L. 50 de la deltamethrine (Decis) chez Megachile rotundata F. Abeille pollinistatrice de la luzerne (Medicago sativa L.) et des effets de doses infralethales sur les adultes et les larves. Apidologie 19 (3): 291-306.

Tesoriero D., Maccagnani B., Santi F., Celli G. (2003) Toxicity of three pesticides on larval instars of Osmia cornuta: preliminary results. Bulletin of Insectology 56: 169-171.

Vicens N., Bosch J. (2000) Nest site orientation and relocation of populations of the orchard pollinator Osmia cornuta (Hymenoptera: Megachilidae). Environmental Entomology 29: 69-75 .

Wasielewski O., Wojciechowicz T., Giejdasz K., Krishnan N. (2013) Overwintering strategies in the red mason solitary bee-physiological correlates of midgut metabolic activity and turnover of nutrient reserves in females of Osmia bicornis. Apidologie 44: 642-656.

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