Changes in the Antioxidative System of the Red Mason Bee (Osmia Rufa) (Hymenoptera: Megachilidae) Induced by Artificially Elongated Diapause

Open access

Changes in the Antioxidative System of the Red Mason Bee (Osmia Rufa) (Hymenoptera: Megachilidae) Induced by Artificially Elongated Diapause

Osmia rufa as a polylectic insect is used in the pollination of numerous plants. The usefulness of this insect for plant breeders is considerably limited because of the short flight periods of the insects in the natural environment. In order to break this limitation, the wintering period of the insects in cocoons is elongated. The temperature is maintained at 4°C up to the time of plant blooming. This treatment does shortens the lifetime of the insects which may be the result of oxidative stress. Such results led to the examination of the selected components of antioxidative system. These components are: total antioxidative status, content of glutathione and activity of peroxidase and glutathione S-transferase in O. rufa emerged in spring, according to their biological clock, and emerged in summer - after elongated diapause. It was observed that diapause elongation unprofitably influenced the antioxidative system of a bee. A statistically significant decrease in total antioxidative status, and activity of both antioixidative enzymes - peroxidase and glutathione S-transferase, was noted. The changes in the glutathione level in turn, were insignificant. In our opinion, the lower efficiency of antioxidative systems of the red mason bee emerged in summer, may limit their response ability to endo- and exogenous factors influencing oxidative stress. The results of our study are the first publication concerning an antioxidative system in mason bees.

Bartosz G. (2003) - Druga twarz tlenu. Wydawnictwo Naukowe PWN

Bosch J., Kemp W. P. (2003) - Effect of wintering duration and temperature on survival and emergence time in males of the orchard pollinator Osmia lingaria (Hymenoptera: Megachilidae). Envir. Entomol., 32: 711-716.

Bosch J., Kemp W. P. (2004) - Effect of pre-wintering and wintering temperature regimes on weight loss, survival, and emergence time in the mason bee Osmia cornuta (Hymenoptera: Megachilidae). Apidologie, 35: 469-479.

Chance B., Maehly A. C. (1955) - Assay of catalases and peroxidases, in: Colowick S.P. and Kaplan N. (Ed.) Methods in Enzymology, Vol. 2. Academic Press, Inc., New York, pp: 764-775.

Chien C., Motoyama N., Dauterman W. C. (1995) - Separation of multiple forms of acidic glutathione S-transferase isozymes in a susceptible and a resistant strain of house fly, Musca domestica (L.). Arch. Insect Biochem. Physiol., 28: 397-406.

Clark A. G. (1990) - The glutathione S-transferases and resistance to insecticides: in Hayes J. D., Pickett C. B. and Mantle T. J. (Ed.) Glutathione S-transferases and Drug Resistance, Taylor and Francis, London. pp. 369-378.

Collins A. M., Williams V., Evans J. D. (2004) - Sperm storage and antioxidative enzyme expression in the honey bee, Apis mellifera. Insect Mol. Biol., 13: 141-146.

Després L., David J. P., Gallet C. (2007) - The evolutionary ecology of insect resistance to plant chemicals. Trends Ecol. Evol., 22: 288-307.

Ellman G. L. (1959) - Tissue sulfyhydryl groups. Arch. Biochem. Biophys., 82: 70-77.

Farjan M. (2008) - Wpływ askorbinianu na metabolism cukrowy i wskaźniki antyoksydacyjne w rozwoju czerwia pszczoły miodnej (Apis mellifera carnica) nieporażonego i porażonego Varroa destructor. Doctoral thesis. University of Warmia and Mazury.

Feng Q. I., Davey K. G., Pang A. S. D., Ladd T. R., Ratnakaran A., Tomkins B., Zheng S., Palli S. (2001) - Developmental expression and stress induction of glutathione S-transferase in the spruce budworm, Christoneura fumiferana. J. Insect. Physiol., 47: 1-10.

Francis F., Haubruge E., Dierickx P. (2002) - Glutathione S-transferase izoenzymes in the two-spot ladybird, Adalia bipunctata (Coleoptera: Coccinellidae). Arch. Insect Biochem. Physiol., 49: 158-166.

Fröhlich D. R., Burris T. E., Brindley W.A. (1989) - Characterization of glutathion-S-transferase in a solitary bee, Megachile rotunda (Fab.) (Hymenoptera: Megachilidea) and inhibition by chalcones, flavones, quercetin and triphanediol. Comp. Biochem. Physiol., 94: 661-665.

Giejdasz K., Wilkaniec Z. (1998) - Effect of the activation of the bee Osmia rufa L., Megachilidae on the emerging dynamics of imagines and their survival rate. Pszczeln. Zesz. Nauk., 42: 265 - 269.

Hayaoka T., Dauterman W. C. (1982) - Induction of glutathione S-transferase by phenobarbital and pesticides in various house fly strains and its effect on toxicity. Pestic. Biochem. Physiol., 17: 113-119.

Joanisse D. R., Storey K. B. (1996) - Oxidative stress and antioxidants in overwintering larvae of cold-hardy goldenrod gall insects. J. Exp. B., 199: 1483-1491.

Jovanovic-Galovic A., Blagojevic D., Grubor-Lajsic G., Worland R., Spasic M. B. (2004) - Role of antioxidant defenseduring different stages of preadult life cycle in European corn borer (Ostrinia nubilalis, Hübn.): diapause and metamorphosis. Arch. Insect Biochem. Physiol., 55: 79-89.

Kafel A., Bednarska K., Augustyniak M., Witas I., Szulińska E. (2003) - Activity of glutatione S-transferase in Spodoptera exigua larvae exposed to cadmium and zinc in two subsequent generations. Environ. Int., 28: 683-686.

Lee K., Berenbaum M. R. (1992) - Ecological aspects of antioxidant enzymes and glutathione-S-transferases in three Papilio species. Biochem. Sys. Ecol., 3: 197-207.

Lipiński Z., Farjan M., Żółtowska K., Polaczek B. (2008) - Effects of dietary transgenic Bt maize pollen on hive worker honeybees. Polish J. of Environ. Stud., 17: 957-961.

Maeta Y., Kurihara M. (1971) - Anatomical and histological studies on the oogenesis and oosorption of terminal oocytes within the genus Osmia. Kontyu, 39: 138-158.

Oakeshott J. G., Johnson R. M., Berenbaum M. R., Ranson H., Cristino A. S., Claudianos C. (2010) - Metabolic enzymes associated with xenobiotic and chemosensory responses in Nasonia vitripennis. Insect Mol. Biol., 19: 147-163.

Papadopoulos A.I., Polemitou I., Laifi P., Yiangou A., Tananaki C. (2004) - Glutathione S-transferase in the insect Apis mellifera macedonica: Kinetic characteristics and effect of stress on the expression of GST isoenzymes in the adult worker bee. Comp. Biochem. Physiol., Part B: 139: 93-97.

Rice-Evans C. A., Diplock A. T., Symons M. C. R. (1991) - Techniques in Free Radical Research, Elsevier, London, New York, Tokyo.

Re R., Pellegrini N., Proteggen A., Pannala A., Yang M., Rice-Evans C. (1999) - Antioxidant activity applying and improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 26: 1231-1237.

Sgolastra F., Bosch J., Molowny-Horas R., Maini S., Kemp W. P. (2010) - Effect of temperature regime on diapause intensity in an adult-wintering Hymenopteran with obligate diapause. J. Insect Physiol., 56: 185-194.

Storey K. B. (1990) - Life in limbo: insights into the biochemical regulation of dormancy. Biochem. Cell. Biol., 68: 401-403.

Wasielewski O., Giejdasz K., Wojciechowicz T., Skrzypski M. (2011) - Ovary growth and protein levels in ovary and fat body during adult-wintering period in the red mason bee, Osmia rufa. Apidologie, 42: 749-758.

Weinhold L. C., Ahmad S., Pardini R. S. (1990) - Insect glutathione S-transferase: a predictor of allelochemical and oxidative stress. Comp. Biochem. Physiol., Part B: 95: 355-363.

Weirich G. F., Collins A. M., Williams V.P. (2002) - Antioxidant enzymes in the honey bee, Apis mellifera. Apidologie, 33: 3-14.

Whiteley G. S. W., Fuller B. J., Hobbs K. E. F. (1992) - Lipid peroxidation in liver tissue specimens stored at subzero temperatures. Cryo-Letters, 13: 83-86.

Wilce M. C., Parker M. W. (1994) - Structure and function of glutathione S-transferase. Biochem. Biophysis. Acta, 1205: 1-18.

Wilkaniec Z. (1991) - Możliwość zastosowania Osmia rufa L. (Apoidea, Magachilidae) w zapylaniu niektórych roślin uprawnych. Rocz. AR Pozn., 229: 173-179.

Yu S. J. (1992) - Plant-allelochemical-adapted glutathione transferases in Lepidoptera, in: Mullin C.A., Scott J.G. (Ed.) Molecular Mechanisms of Insecticide Resistance, American Chemical Society, Washington DC, pp: 174-190.

Yu S. J. (1996) - Insect glutathione S-Transferases. Zool. Stud., 35: 9-19.

Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

Journal Information


IMPACT FACTOR 2017: 0.75
5-year IMPACT FACTOR: 1.007

CiteScore 2017: 0.92

SCImago Journal Rank (SJR) 2017: 0.345
Source Normalized Impact per Paper (SNIP) 2017: 0.461

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 149 149 12
PDF Downloads 58 58 8