Genetic variation of Apis mellifera from Serbia inferred from mitochondrial analysis

Open access

Genetic variation of Apis mellifera from Serbia inferred from mitochondrial analysis

Two honeybee subspecies inhabit Serbia; Apis mellifera carnica and A. m. macedonica. Both belong to eastern Mediterranean (C) evolutionary lineage. Furthermore three Serbian honeybee ecotypes restricted to particular regions, were defined through morphometry and cytogenetic analyses. In this study, mitochondrial data have been used to analyze the molecular diversity of the honeybee population from Serbia. Seven haplotypes of the C evolutionary lineage have been found, two of them are newly described (C2o and C2p) and restricted to two regions, which ultimately increased the number of haplotypes found in this lineage. Comparisons with surrounding honeybee populations suggest a hybrid situation between A. m. carnica and A. m. macedonica and also introgression from A. m. ligustica. The results should be considered when dealing with future conservation strategies, and for pathogen-parasite-tolerant breeding programs.

Bandelt H. J., Forster P., Rōhl A. (1999) - Median-joining networks for inferring intraspecific phylogenies. Mol. Biol. Evol., 16: 37-48.

Bouga M., Harizanis P. B., Kilias G., Alahiotis S. (2005) - Genetic divergence and phylogenetic relationships of honey bee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR - RFLP analysis of three mtDNA segments. Apidologie, 36: 335-344.

Cirkovic D. (2002) - Reproductive-productive and hygienic-grooming characterization of Syenichko-Peshterski honey bee ecotype, MSc thesis, Belgrade University.

Crozier R. H., Crozier Y. H. (1993) - The mitochondrial genome of the honey bee Apis mellifera: complete sequence and genome organisation. Genetics, 133: 97-117.

Dedej S., Delaplane K. S., Gocaj E. (2000) - A technical and economic evaluation of beekeeping in Albania. Bee World, 81: 87-97.

Engel M. S. (1999) - The taxonomy of recent and fossil honey bees (Hymenoptera: Apidae, Apis). J. Hymenop. Res., 8: 165-196.

Franck P., Garnery L., Celebrano G., Solignac M., Cornuet J. M. (2000) - Hybrid origins of honey bees from Italy (Apis mellifera ligustica) and Sicily (A. m. sicula). Mol. Ecol., 9: 907-921.

Garnery L., Vautrin D., Cornuet J. M., Solignac M. (1991) - Phylogenetic relationships in the genus Apis inferred from mitochondrial DNA sequence data. Apidologie, 22: 87-92.

Hewitt G. M. (1999) - Post-glacial recolonization of European biota. Biol. J. Linn. Soc., 68: 87-112.

Ivanova E., Petrov P., Bouga M., Emmanouel N., Ivgin-Tukka R., Kence M. (2010) - Genetic variation in honey bee (Apis mellifera L.) populations from Bulgaria. J. Apic. Sci., 54: 49-59.

KaToh K., Toh H. (2008) - Recent developments in the MAFFT multiple sequence alignment program. Briefings in Bioinformatics, 9: 286-298.

Martimianakis S., Klossa-Kilia E., Bouga M., Kilias G. (2011) - Phylogenetic relationships of Greek Apis mellifera subspecies based on sequencing of mtDNA segments (COI and ND5). J. Apic. Res., 50: 42-50.

Moritz R. F. A., Härtel S., Neumann P. (2005) - Global invasions of the western honey bee (Apis mellifera) and the consequences for biodiversity. Ecoscience, 12: 289-301.

Muñoz I., Dall'Olio R., Lodesani M., De la Rúa P. (2009) - Population genetic structure of coastal Croatian honeybees (Apis mellifera carnica). Apidologie, 40: 617-626.

Özdil F., Yildiz M. A., Hall H. G. (2009) - Molecular characterization of Turkish honeybee populations (Apis mellifera L.) inferred from mitochondrial DNA RFLP and sequence results. Apidologie, 40: 570-576.

Pejovic D. (2001) - Investigations of hygienic and grooming behaviour of honey bee subspecies Apis mellifera carnica in disease resistance, MSc thesis, Belgrade University.

Ruttner F. (1988) - Biogeography and Taxonomy of Honeybees, Springer-Verlag; Berlin, Germany, 284 pp.

Sheppard W. S., Meixner M. D. (2003) - Apis mellifera pomonella, a new honey bee subspecies from Central Asia. Apidologie, 34: 367-375.

Sinacori A., Rinderer T. E., Lancaster V., Sheppard W. S. (1998) - A morphological assessment of Apis mellifera from Palermo, Italy. Apidologie, 29: 481-490.

Stanimirovic Z., Popeskovic D., Pejovic D. (1999a) - Biodiversity of the honeybee Apis mellifera, Linne (1758), from the Yugoslav regions I - the biometric variability of the Banate and Sjenicko-Peshtersky ecotypes chromosomes. Acta Vet. Beograd, 49: 199-206.

Stanimirovic Z., Vucinic M., Stevanovic J. (1999b) - Biodiversity of the honeybee Apis mellifera, Linne (1758), from some Yugoslav regions: II - Ultrastructural chromosomal differences between Banat and Syenichko-Peshterski honeybee ecotype. Acta Vet. Beograd, 49: 207-214.

Stanimirovic Z., Pejovic D., Stevanovic J., Vucinic M., Mirilovic M. (2002) - Investigations of hygienic behaviour and disease resistance in organic beekeeping of two honeybee ecogeographic varieties from Serbia. Acta Vet. Beograd, 52: 169-180.

Stanimirovic Z., Stevanovic J., Andjelkovic M. (2005a) - Chromosomal diversity in Apis mellifera carnica from Serbia. Apidologie, 36: 31-42.

Stanimirovic Z., Stevanovic J., Cirkovic D. (2005b) - Behavioural defenses of the honey bee ecotype from Sjenica - Pester against Varroa destructor. Acta Vet. Beograd, 55: 69-82.

Stevanovic J. (2002) - Investigations of morphometric and chromosomal variability in diversity preserving of Carniolan honey bee (Apis mellifera carnica Pollmann, 1879) in Serbia, MSc thesis, Belgrade University.

Stevanovic J. (2007) - Ecological-ethological defence mechanisms of Apis mellifera carnica against ectoparasite Varroa destructor on the territory of Serbia. PhD dissertation, PhD thesis, Belgrade University.

Stevanovic J., Stanimirovic Z., Radakovic M., Kovacevic R. S. (2010) - Biogeographic study of the honey bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia based on mitochondrial DNA analyses. Rus. J. Genet., 46: 603-609.

Stevanovic J., Stanimirovic Z., Genersch E., Kovacevic S. R., Ljubenkovic J., Radakovic M., Aleksic N. (2011) - Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder. Apidologie, 42: 49-58.

Sušnik S., Kozmus P., Poklukar J., Meglič V. (2004) - Molecular characterisation of indigenous Apis mellifera carnica in Slovenia. Apidologie, 35: 623-636.

Walsh P. S., Metzqer D. A., Higuchi R. (1991) - Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques, 10: 506-512.

Whitfield C. W., Behura S. K., Berlocher S. H., Clark A. G., Johnston J. S., Sheppard W. S., Smith D. R., Suarez A. V., Weaver D., Tsutsui N. D. (2006) - Thrice out of Africa: Ancient and recent expansions of the honey bee, Apis mellifera. Science, 314: 642-645.

Journal of Apicultural Science

The Journal of Research Institute of Horticulture and Apicultural Research Association

Journal Information


IMPACT FACTOR 2017: 0.75
5-year IMPACT FACTOR: 1.007

CiteScore 2017: 0.92

SCImago Journal Rank (SJR) 2017: 0.345
Source Normalized Impact per Paper (SNIP) 2017: 0.461

Cited By

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 249 249 30
PDF Downloads 119 119 23