Biochemical markers for the assessment of aquatic environment contamination

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Biochemical markers for the assessment of aquatic environment contamination

The need for assessment of aquatic ecosystem contamination and of its impact on water dwelling organisms was developed in response to rising aquatic environmental pollution. In this field study, liver enzymes of phase I and phase II of xenobiotic transformation, namely cytochrome P450, ethoxyresorufin-O-deethylase, glutathione-S-transferase and tripeptide glutathione were used to assess the contamination of the aquatic environment at different rivers in the Czech Republic. The indicator species selected was the male chub (Leuciscus cephalus L.) and male brown trout (Salmo trutta fario). Chemical analyses included also the assessment of the most important inductors of previously mentioned biochemical markers. The major inductors of monitored biomarkers are industrial contaminants which belong to a large group of organic pollutants (PCB, PAH, PCDD/F, DDT, HCH, HCB and OCS), persistent in the environment. Four different groups of river basins were assessed: the River Tichá Orlice and its tributary the Kralický brook; important tributaries of the River Elbe (the rivers Orlice, Chrudimka, Cidlina, Jizera, Vltava, Ohře and Bílina); major rivers in the Czech Republic (the rivers Lužnice, Otava, Sázava, Berounka, Vltava, Labe, Ohře, Svratka, Dyje, Morava and Odra) and the River Vltava. The use of the biochemical markers together with chemical analyses seems to be an effective way to monitor the quality of aquatic environment.

Al-Arabi SAM, Adolfsson-Erici M, Waagbo R, Ali MS and Goksoyr A. (2005) Contaminant accumulation and biomarker responses in caged fish exposed to effluents from anthropogenic sources in the Karnaphuly River, Bangladesh. Environ Toxicol Chem 24: 1968-1978.

Boon JP, Everaarts JM, Hillebrand MTJ, Eggens ML, Pijnenburg J and Goksøyr A. (1992) Changes in levels of hepatic biotransformation enzymes and hemoglobin levels in female plaice (Pleuronectes-platessa) after oral-administration of a technical polychlorinated biphenyl mixture (Clophen A40). Sci Total Environ 114: 113-133.

Bozcaarmutlu A and Arinc E. (2004) Inhibitory effects of divalent metalions on liver microsomal 7-ethoxyresorufin-O-deethylase (EROD) activity of leaping mullet. Mar Environ Res 58: 521-524.

Brammell BF, Price DJ, Birge WJ and Elskus AA. (2004) Apparent lack of CYP1A response to high PCB body burdens in fish from a chronically contaminated PCB site. Mar Environ Res 58: 251-255.

Burke MD and Mayer RT. (1974) Ethoxyresorufin - direct fluorimetric assay of a microsomal o-dealkylation which is preferentially inducible by 3-methylcholanthrene. Drug Metab Dispos 2: 583-588.

Eaton DL and Bammler TK. (1999) Concise review of the glutathione S-transferases and their significance to toxicology. Toxicol Sci 49: 156-164.

Ellman GL. (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82: 70-77.

Flammarion P, Devaux A, Nehls S, Migeon B, Noury P and Garric J. (2002) Multibiomarker responses in fish from the Moselle River (France). Ecotox Environ Safe 51: 145-153.

Forlin L, Haux C, Karlsson-Norrgren L, Runn P and Larsson A. (1986) Biotransformation enzyme-activities and histopathology in rainbow trout, Salmo Gairdneri, treated with cadmium. Aquat Toxicol 8: 51-64.

Foster EP, Fitzpatrick MS, Feist GW, Schreck CB, Yates J, Spitsbergen JM and Heidel JR. (2001) Plasma androgen correlation, EROD induction, reduced, condition factor, and the occurrence of organochlorine pollutants in reproductively immature white sturgeon (Acipenser transmontanus) from the Columbia River, USA. Arch Environ Con Tox 41: 182-191.

Frasco MF and Guilhermino L. (2002) Effects of dimethoate and beta-naphthoflavone on selected biomarkers of Poecilia reticulata. Fish Physiol Biochem 26: 149-156.

Gadagbui BKM and Goksoyr A. (1996) CYP1A and other biomarker responses to effluents from a textile mill in the Volta River (Ghana) using caged tilapia (Oreochromis niloticus) and sediment-exposed mudfish (Clarias anguillaris). Biomarkers 1: 252-261.

Goksoyr A and Forlin L. (1992) The cytochrome-P-450 system in fish, aquatic toxicology and environmental monitoring. Aquat Toxicol 22: 287-311.

Grabic R, Novak J and Pacakova V. (2000) Optimization of a GC-MS/MS method for the analysis of PCDDs and PCDFs in human and fish tissue. HRC-J High Res Chrom 23: 595-599.

Habig WH, Pabst MJ and Jakoby WB. (1974) Glutathione S-transferases. First enzymatic step in mercapturic acid formation. J Biol Chem 249: 7130-7139.

Hajslova J, Schoula R, Holadova K and Poustka J. (1995) Analysis of PCBs in biotic matrices by 2-dimensional GC-ECD. Int J Environ An Ch 60: 163-173.

Hamed RR, Farid NM, Elowa SHE and Abdalla AM. (2003) Glutathione related enzyme levels of freshwater fish as bioindicators of pollution. Environmentalist 23: 313-322.

Havelkova M, Blahova J, Kroupova H, Randak T, Slatinska I, Leontovycova D, Grabic R, Pospisil R and Svobodova Z. (2008a) Biomarkers of contaminant exposure in chub (Leuciscus cephalus L.)-biomonitoring of major Rivers in the Czech Republic. Sensors 8: 2589-2603.

Havelkova M, Randak T, Zlabek V, Krijt J, Kroupova H, Pulkrabova J and Svobodov Z. (2007) Biochemical markers for assessing aquatic contamination. Sensors 7: 2599-2611.

Havelkova M, Svobodova Z, Kolarova J, Krijt J, Nemethova D, Jarkovsky J and Pospisil R. (2008b) Organic pollutant contamination of the river Ticha Orlice as assessed by biochemical markers. Acta Vet Brno 77: 133-141.

Heininger P and Pelzer J. (1998) Trends and patterns in the contamination of sediments from federal waterways in Eastern Germany. Acta Hydroch Hydrob 26: 218-225.

Henczova M, Deer AK, Komlosi V and Mink J. (2006) Detection of toxic effects of Cd2+ on different fish species via liver cytochrome P450-dependent monooxygenase activities and FTIR spectroscopy. Anal Bioanal Chem 385: 652-659.

Henson KL, Stauffer G and Gallagher EP. (2001) Induction of glutathione S-transferase activity and protein expression in brown bullhead (Ameiurus nebulosus) liver by ethoxyquin. Toxicol Sci 62: 54-60.

Hosnedl T, Hajslova J, Kocourek V, Tomaniova M and Volka K. (2003) 1-hydroxypyrene as a biomarker for fish exposure to polycyclic aromatic hydrocarbons. B Environ Contam Tox 71: 465-472.

Koehler HR, Sandu C, Scheil V, Nagy-Petrica EM, Segner H, Telcean I, Stan G and Triebskorn R. (2007) Monitoring pollution in river Mures, Romania, Part III: biochemical effect markers in fish and integrative reflection. Environ Monit Assess 127: 47-54.

Kolarova J, Svobodova Z, Zlabek V, Randak T, Hajslova J and Suchan P. (2005) Organochlorine and PAHs in brown trout (Salmo trutta fario) population from Tichá Orlice River due to chemical plant with possible effects to vitellogenin expression. Fresen Environ Bull 14: 1091-1096.

Krca S, Zaja R, Calic V, Terzic S, Grubesic MS, Ahel M and Smital T. (2007) Hepatic biomarker responses to organic contaminants in feral chub (Leuciscus cephalus) - laboratory characterization and field study in the Sava River, Croatia. Environ Toxicol Chem 26: 2620-2633.

Lehmann A and Rode M. (2001) Long-term behaviour and cross-correlation water quality analysis of the River Elbe, Germany. Water Res 35: 2153-2160.

Lewis DFV. (2001) Guide to cytochromes P450. Structure and function. Taylor & Francis Inc., London.

Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. (1951) Protein measurement with folin phenol reagent. J Biol Chem 193: 265-275.

Mannervik B and Danielson UH. (1988) Glutathione transferases - structure and catalytic activity. Crit Rev Biochem Mol 23: 283-337.

Mayon N, Bertrand A, Leroy D, Malbrouck C, Mandiki SNM, Silvestre F, Goffart A, Thome JP and Kestemont P. (2006) Multiscale approach of fish responses to different types of environmental contaminations: A case study. Sci Total Environ 367: 715-731.

Noble E, Barre H and Dierickx PJ. (1998) Effect of diet and beta-naphthoflavone on hepatic and renal glutathione S-transferase isoenzymes in carp (Cyprinus carpio). Fish Physiol Biochem 18: 203-212.

Otto DME and Moon TW. (1996) Phase I and II enzymes and antioxidant responses in different tissues of brown bullheads from relatively polluted and non-polluted systems. Arch Environ Con Tox 31: 141-147.

Parente TEM, De-Oliveira ACAX and Paumgartten FJR. (2008) Induced cytochrome P450 1A activity in cichlid fishes from Guandu River and Jacarepagua Lake, Rio de Janeiro, Brazil. Environ Pollut 152: 233-238.

Perez-Lopez M, Anglade P, Bec-Ferte MP, Debrauwer L, Perdu E, Cravedi JP and Rouimi P. (2000) Characterization of hepatic and extrahepatic glutathione S-transferases in rainbow trout (Oncorhynchus mykiss) and their induction by 3,3',4,4'-tetrachlorobiphenyl. Fish Physiol Biochem 22: 21-32.

Perez-Lopez M, Novoa-Valinas MC and Melgar-Riol MJ. (2002) Glutathione S-transferase cytosolic isoforms as biomarkers of polychlorinated biphenyl (Arochlor 1254) experimental contamination in rainbow trout. Toxicol Lett 136: 97-106.

Petrivalsky M, Machala M, Nezveda K, Piacka V, Svobodova Z and Drabek P. (1997) Glutathione-dependent detoxifying enzymes in rainbow trout liver: Search for specific biochemical markers of chemical stress. Environ Toxicol Chem 16: 1417-1421.

Randak T, Zlabek V, Kolarova J, Svobodova Z, Hajslova J, Siroka Z, Janska M, Pulkrabova J, Cajka T and Jarkovsky J. (2006) Biomarkers detected in Chub (Leuciscus cephalus L.) to evaluate contamination of the Elbe and Vltava rivers, Czech Republic. B Environ Contam Tox 76: 233-241.

Schlezinger JJ and Stegeman JJ. (2001) Induction and suppression of cytochrome P4501A by 3,3',4,4',5-pentachlorobiphenyl and its relationship to oxidative stress in the marine fish scup (Stenotomus chrysops). Aquat Toxicol 52: 101-115.

Schmidt K, Steinberg CEW, Pflugmacher S and Staaks GBO. (2004) Xenobiotic substances such as PCB mixtures (Aroclor 1254) and TBT can influence swimming behavior and biotransformation activity (GST) of carp (Cyprinus carpio). Environ Toxicol 19: 460-470.

Sen A and Semiz A. (2007) Effects of metals and detergents on biotransformation and detoxification enzymes of leaping mullet (Liza saliens). Ecotox Environ Safe 68: 405-411.

Siroka Z, Krijt J, Randak T, Svobodova Z, Peskova G, Fuksa J, Hajslova J, Jarkovsky J and Janska M. (2005) Organic pollutant contamination of the River Elbe as assessed by biochemical markers. Acta Vet Brno 74: 293-303.

Slatinska I, Blahova J, Siroka Z, Krijt J, Svobodova Z, Randak T, Nemethova D, Jarkovsky J and Havelkova M. (2008) Use of biochemical markers for the assessment of organic pollutant contamination of the River Vltava, Czech Republic. Ecotox Environ Safe (in press)

Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ and Klenk DC. (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76-85.

Stegeman JJ and Hahn ME. (1994) Biochemistry and molecular biology of monooxygenase: current perspective on forms, functions, and regulation of cytochrome P450 in aquatic species, in Aquatic toxicology; Molecular, Biochemical and Cellular Perspectives (Malins DC and Ostrander GK eds), pp. 87-206, Lewis Publishers, CRC press, Boca Raton.

Stegeman JJ, Woodin BR, Singh H, Oleksiak MF and Celander M. (1997) Cytochromes P450 (CYP) in tropical fishes: catalytic activities, expression of multiple CYP proteins and high levels of microsomal P450 in liver of fishes from Bermuda. Comp Biochem Phys 116C: 61-75.

Stien X, Risso C, Gnassiabarelli M, Romeo M and Lafaurie M. (1997) Effect of copper chloride in vitro and in vivo on the hepatic EROD activity in the fish Dicentrarchus labrax. Environ Toxicol Chem 16: 214-219.

Svobodova Z, Celechovska O, Kolarova J, Randak T and Zlabek V. (2004) Assessment of metal contamination in the upper reaches of the Tichá Orlice River. Czech J Anim Sci 49: 458-464.

Van den Berg M, Birnbaum L, Bosveld ATC, Brunström B, Cook P, Feeley M, Giesy JP, Hanberg A, Hasegawa R, Kennedy SW, Kubiak T, Larsen JC, van Leeuwen FXR, Liem AKD, Nolt C, Peterson RE, Poellinger L, Safe S, Schrenke D, Tillitt D, Tysklind M, Younes M, Wærn F and Zacharewski T. (1998) Toxic equivalency factors (TEFs) for PCBs, PCDDs, PCDFs for humans and wildlife. Environ Health Persp 106: 775-792.

Van der Oost R, Beyer J and Vermeulen NPE. (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ Toxicol Phar 13: 57-149.

Vanderoost R, Vangastel L, Worst D, Hanraads M, Satumalay K, Vanschooten FJ, Heida H and Vermeulen NPE. (1994) Biochemical markers in feral roach (Rutilus rutilus) in relation to the bioaccumulation of organic trace pollutants. Chemosphere 29: 801-817.

Vigano L, Arillo A, Melodia F, Bagnasco M, Bennicelli C and Deflora S. (1995) Hepatic and biliary biomarkers in rainbow-trout injected with sediment extracts from the River Po (Italy). Chemosphere 30: 2117-2128.

Vindimian E, Namour P, Migeon B and Garric J. (1991) Insitu pollution induced cytochrome P450 activity offresh-water fish - barbel (Barbus barbus), chub (Leuciscus cephalus) and nase (Chondrostom nasus). Aquat Toxicol 21: 255-266.

Whyte JJ, Jung RE, Schmitt CJ and Tillitt DE. (2000) Ethoxyresorufin-O-deethylase (EROD) activity in fish as a biomarker of chemical exposure. Crit Rev Toxicol 30: 347-570.

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The Journal of Institute of Experimental Pharmacology of Slovak Academy of Sciences

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