Dehydrogenase activity of forest soils depends on the assay used

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Abstract

Dehydrogenases are exclusively intracellular enzymes, which play an important role in the initial stages of oxidation of soil organic matter. One of the most frequently used methods to estimate dehydrogenase activity in soil is based on the use of triphenyltetrazolium chloride as an artificial electron acceptor. The purpose of this study was to compare the activity of dehydrogenases of forest soils with varied physicochemical properties using different triphenyltetrazolium chloride assays. The determination was carried out using the original procedure by Casida et al., a modification of the procedure which involves the use of Ca(OH)2 instead of CaCO3, the Thalmann method, and the assay by Casida et al. without addition of buffer or any salt. Soil dehydrogenase activity depended on the assay used. Dehydrogenase determined by the Casida et al. method without addition of buffer or any salt correlated with the pH values of soils. The autoclaved strongly acidic samples of control soils showed high concentrations of triphenylformazan, probably due to chemical reduction of triphenyltetrazolium chloride. There is, therefore, a need for a sterilization method other than autoclaving, ie a process that results in significant changes in soil properties, thus helping to increase the chemical reduction of triphenyltetrazolium chloride.

Alef K. and Nannipieri P., 1995. Enzyme activities. In: Methods in applied, Soil Microbiology and Biochemistry (Eds K.Alef, P. Nannipieri). Academic Press, London-New York- San Francisco.

Benefield C.B., Howard P.J.A., and Howard D., 1977. The estimation of dehydrogenase activity, Soil Biol. Biochem., 9, 67-70.

Berns A.E., Phlilipp H., Narres H.D., Burauel P., Vereecken P., and Tappe W., 2008. Effect of gamma-sterilization and autoclaving on soil organic matter structure as studied by solid state NMR, UV and fluorescence sopectroscopy. Eur.J. Soil Sci., 59, 540-550.

Beyer L., Wachendorf C., Elsne D.C., and Knabe R., 1993. Suitability of dehydrogenase activity assay as an index of soil bilogical activity, Biol. Fertil. Soil, 16, 52-56.

Brookes P.C., 1995. The use of microbial parameters in monitoring soil pollution by heavy metals. Biol. Fertil. Soil, 19, 269-275.

Brzezińska M., Stępniewska Z., and Stępniewski W., 2001. Dehydrogenase and Catalase Activity of Soil Irigated with Municipal Wastewater, Polish J. Environ. Studies, 10, 307-311.

Camiña F., Trasar-Cepeda C., Gil-Sotres F., and Leirós C., 1998. Measurement of dehydrogenase activity in acid soils rich in organic matter. Soil Biol. Biochem., 30, 1005-1011.

Casida L.E., Klein D.A., and Santoro T., 1964. Soil dehydrogenase activity. Soil Sci., 98, 371-376.

Chodak M., Pietrzykowski M., and Niklińska M., 2009. Development of microbial properties in a chronosequence of sandy mine soils. Applied Soil Ecol., 41, 259-268.

Friedel J., Mölter K., and Fisher W., 1994. Comparison and improvement of methods for determining soil dehydrogenase activity by using triphenylotetrazolium chloride and iodonitrotetrazolium chloride. Biol. Fertil. Soil, 18, 291-296.

Gajda A.M., Przewłoka B., and Gawryjołek K., 2013. Changes in soil quality associated with tillage system applied. Int. Agrophysics, 27, 133-141.

Ghaly A.E. and Mahmoud N.S., 2007. Effect of tetrazolium chloride concentration, O2, and cell age on dehydrogenase activity of Aspergillus niger. Appl. Biochem. Biotechnol., 136, 207-222.

Gong P., 1997. Dehydrogenase activity in soil: a comparison between the TTC and INT assay under their optimum conditions, Soil Biol. Biochem., 29, 211-214.

Januszek K., Błońska E., and Stanik P., 2007. Comments concerning determination of dehydrogenase activity in soil by the TTC-Formazan test (in Polish). Acta Agrophysica, 9(3), 635-644.

Karr D.B. and Emerich D.W., 2000. Bradyrhizobium japonicum Isocitrate Dehydrogenase exibit calcium-dependent hysteresis. Arch. Biochem. Biophysics, 376(1), 101-108.

Klose S., Acosta-Martínez V., and Ajwa H.A., 2006. Microbial community composition and enzyme activities in a sandy loam soil after fumigation with metyl bromide or alternative biocides. Soil Biol. Biochem., 38, 1243-1254.

Lenhard V.G., 1956. Die Dehydrogenaseaktiwität des Bodens als Mass für die Microorganismentätigkeit im Boden. Z Pflanzenernähr Bodenkd., 73, 1-11.

Lityński T., Jurkowska H., and Gorlach E., 1976. Chemical- Agricultural Analysis (in Polish). PWN, Warsaw, Poland.

Malkomes H.P., 1991. Vergleich der TTC- und INT-reduction zum nachweis von pflanzenschutzmittelwirkungen auf die dehydrogenaseaktivität im boden. Nachrichtenbl. Dtsch. Pflanzenschutzd, 43, 52-57.

Nannipieri P., Grego S., and Cecanti B., 1990. Ecological significance of the biological activity in soil. In: Soil Biochem. (Eds J.M. Bollag, B. Stotzky). Dekker, New York, USA.

Paradelo R. and Barral M.T., 2009. Effect of moisture and disaggregation on the microbial activity of soil, Soil Till. Res., 104(2), 317-319.

Praveen-Kumar B. and Tarafdar J.C., 2003. 2,3,5-Triphenyltetrazolium chloride (TTC) as an electron acceptor of culturable soil bacteria, fungi and actinomycetes. Biol. Fertil. Soil, 38, 186-189.

Quilchano C. and Marañón T., 2002. Dehydrogenase activity in Mediterranean forest soils. Biol. Fertil. Soil, 35, 102-107.

Ross D.J., 1971. Some factors influencing the estimation of dehydrogenase activities of some soils under pasture. Soil Biol. Biochem., 3, 97-110.

Rossel D. and Tarradellas J., 1991. Dehydrogenase activity of soil micloflora: significance in ecotoxicological tests. Environ. Toxicol Water Qual., 6, 17-33.

Rous J., Brookes P.C., and Bååth E., 2010. Investigating the mechanisms for the opposing pH relationships of fungal and bacterial growth in soil. Soil Biol. Biochem., 42, 926-934.

Shaw L.J. and Burns R.G., 2006. Enzyme Activity Profiles and Soil Quality. In: Microbiological Methods for Assessing Soil Quality (Eds J. Bloem, D.W. Hopkins, A. Benedetti). CABI Publishing, London, UK.

Skawryło-Bednarz B. and Krzepiłko A., 2009. Effect of different fertilization on enzyme activity in rhizosphere and nonrhizosphere of amaranth. Int. Agrophys., 23, 409-412.

StatSoft Inc., 2011. STATISTICA Version 10, Computer software.

Stevenson I.L., 1959. Dehydrogenase activity in soils. Can. J. Microbiol., 5, 97-110.

Stępniewska Z., 1987. Fe2+ interference in determination of dehydrogenase activity of soils. Polish J. Soil Sci. Soil Chem., 21(1), 25-31.

Trasar-Cepeda C., Leiros M.C., Seoane S., and Gil-Sotres F., 2000. Limitations of soil enzymes as indicators of soil pollution. Soil Biol. Biochem., 32, 1867-1875.

Trevors J.T., 1984. Effect of substrate concentration, inorganic nitrogen, O2 concentration, temperature and pH on dehydrogenase activity in soil. Plant Soil, 77, 285-293.

Von Mersi W. and Schinner F., 1991. An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chloride. Biol. Fertil. Soils, 11, 216-220.

Włodarczyk T. Stępniewski W., and Brzezińska M., 2002. Dehydrogenase activity, redox potential, and emissions of carbon dioxide and nitrous oxide from Cambisols under flooding condition. Biol. Fertil. Soils, 36, 200-2006.

Wolińska A., 2010. Dehydrogenase activity of soil microorganisms and oxygen availability during reoxidation process of selected mineral soils from Poland (in Polish). Acta Agrophysica Monographiae, 3, 1-88.

WRB, 2006. World reference base for soil resources. A framework for international classification, correlation and communication. World Soil Resources Reports 103. FAO UN, Rome.

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