Objective To investigate the prevalence and levels of anti-HEV IgG in the population of Jiangsu Province.
Methods Total of 2 656 samples from Qindong and 11 463 samples from Anfeng were colleted. The anti- HEV antibody was qualitatively and quantitatively detected using ELISA kits and the references had been established.
Results The positive rates of anti-HEV IgG in male and female were 55.6% and 40.1%, respectively. The positive rate of anti-HEV IgM in male and female were both 3.4%. In opposite to anti-HEV IgG, the positive rate of anti-HEV IgM in Anfeng was significant higher than that in Qindong. The mean anti-HEV IgG titers for 6 age groups were 0.94, 0.92, 1.07, 1.46, 1.27, 1.19 and 0.68, 1.31, 1.08, 1.14, 1.31, 1.68 IU/ml, in Qindong and Anfeng region, respectively. The positive rate of anti-HEV IgG tended to increase with age and the titer of anti- HEV IgG was associated with age (R > 0.90).
Conclusions The results in this study showed that HEV was widely prevalent in both Qindong and Anfeng of Jiansu Province and the prevalence and the anti-HEV IgG titer were associated with gender and age.
1 Teshale EH, Hu DJ, Holmberg SD. The two faces of hepatitis E virus. Clin Infect Dis 2010; 51:328-334.
2 Aggarwal R, Naik S. Epidemiology of hepatitis E: current status. J Gastroenterol Hepatol 2009; 24: 1484-1493.
3 Dalton HR, Thurairajah PH, Fellows HJ, Hussaini HS, Mitchell J, Bendall R, et al. Autochthonous hepatitis E in southwest England. J Viral Hepat 2007;14:304-309.
4 Khuroo MS, Kamili S. Aetiology, clinical course and outcome of sporadic acute viral hepatitis in pregnancy. J Viral Hepat 2003;10:61-69.
5 Teshale EH, Howard CM, Grytdal SP, Handzel TR, Barry V, Kamili S, et al. Hepatitis E epidemic, Uganda. Emerg Infect Dis 2010; 16:126-129.
6 Purcell RH, Emerson SU. Hepatitis E: an emerging awareness of an old disease. J Hepatol 2008; 48:494-503.
7 Takahashi K, Iwata K, Watanabe N, Hatahara T, Ohta Y, Baba K, et al. Full-genome nucleotide sequence of a hepatitis E virus strain that may be indigenous to Japan. Virology 2001; 287:9-12.
8 Bryan JP, Tsarev SA, Iqbal M, Ticehurst J, Emerson S, Ahmed A, et al. Epidemic hepatitis E in Pakistan: patterns of serologic response and evidence that the antibody to hepatitis E virus protects against disease. J Infect Dis 1994;170:517-521.
9 Tsarev SA, Tsareva TS, Emerson SU, Govindarajan S, Shapiro M, Gerin JL, et al. Successful passive and active immunization of cynomolgus monkeys against hepatitis E. Proc Natl Acad Sci USA 1994; 91:10198-10202.
10 Ai X, Zhang XF, Huang SJ, Jiang HM, Wang YJ, Yang CL, et al. Analysis on the epidemiological characteristics of the sporadic cases with hepatitis E infection in the rural area in Jiangsu Province. Zhongguo Ren Shou Gong Huan Bing Xue Bao (Chin) 2009;25:253-256.
11 Li RC, Ge SX, Li YP, Zheng YJ, Nong Y, Guo QS, et al. Seroprevalence of hepatitis E virus infection, rural southern People’s Republic of China. Emerg Infect Dis 2006;12:1682-1688.
12 Zhang XF, Zhang J , Liu SL, Wu YS, Meng FY, Zhu FC. Seroepidemiological study on hepatitis E virus infection in rural general population in Jiangsu Province. Zhongguo Tian Ran Yao Wu (Chin) 2007; 19:53-55.
13 Zhou C, Huang WJ, Yao X, Yan Q, Zhang J, Zhu FC, et al. Evaluation of the diagnostic kits for hepatitis E and establishment of a quantification method for detecting anti-HEV IgG. Zhonghua Wei Sheng Wu Xue Mian Yi Xue Za Zhi (Chin) 2009; 29:854-857.
14 Jang JH, Jung YM, Kim JS, Lee SH, Kim JW, Hwang SG, et al. Coexistence of IgM antihepatitis A virus and IgM antihepatitis E virus in acute viral hepatitis: a prospective, multicentre study inKorea. J Viral Hepat 2011; 18:408-414.
15 Ji Y, Zhu Y, Liang J, Wei X, Yang X, Wang L, et al. Swine hepatitis E virus in rural southern China: genetic characterization and experimental infection in rhesus monkeys (Macaca mulatta). J Gastroenterol 2008; 43:565-570.
16 Zhang J, Ge SX, Huang GY, Li SW, He ZQ, Wang YB, et al. Evaluation of antibody-based and nucleic acid-based assays for diagnosis of hepatitis E virus infection in a rhesus monkey model. J Med Virol 2003;71:518-526.
17 Ijaz S, Vyse AJ, Morgan D, Pebody RG, Tedder RS, Brown D. Indigenous hepatitis E virus infection in England: more common than it seems. J Clin Virol 2009; 44:272-276.
18 Minuk GY, Sun A, Sun DF, Uhanova J, Nicolle LE, Larke B, et al. Serological evidence of hepatitis E virus infection in an indigenous North American population. Can J Gastroenterol 2007; 21:439-442.
19 Labrique AB, Zaman K, Hossain Z, Saha P, Yunus M, Hossain A, et al. Population seroprevalence of hepatitis E virus antibodies in rural Bangladesh. Am J Trop Med Hyg 2009; 81:875-881.
20 Zheng Y, Ge S, Zhang J, Guo Q, Ng MH, Wang F, et al. Swine as a principal reservoir of hepatitis E virus that infects humans in eastern China. J Infect Dis 2006; 193:1643-1649.
21 Khuroo MS, Kamili S, Dar MY, Moecklii R, Jameel S. Hepatitis E and long-term antibody status. Lancet 1993; 341:1355.
22 Myint KS, Endy TP, Shrestha MP, Shrestha SK, Vaughn DW, Innis BL, et al. Hepatitis E antibody kinetics in Nepalese patients. Trans R Soc Trop Med Hyg 2006;100:938-941.
23 Innis BL, Seriwatana J, Robinson RA, Shrestha MP, Yarbough PO, Longer CF, et al. Quantitation of immunoglobulin to hepatitis E virus by enzyme immunoassay. Clin Diagn Lab Immunol 2002; 9:639-648.
24 Pourpongporn P, Samransurp K, Rojanasang P, Wiwattanakul S, Srisurapanon S. The prevalence of anti-hepatitis E in occupational risk groups. J Med Assoc Thai 2009; 92:38-42.