Inflammatory Cytokine Secretion Status of Bone Marrow Cells and Clinical Significance in Immunerelated Hematocytopenia

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Abstract

Objective To observe the expression of inflammatory molecules in bone marrow immune cells of patients with immune-related hematocytopenia (IRH), and to investigate the immune mechanism and clinical significance of the disease.

Methods Total of 36 IRH patients were selected as observation group and 30 healthy people were taken as control group. Serum cytokines levels, activity of immunocytes and expression of HLA-DR were detected. Immune fluorescence was applied to observe the expression state of immunologic molecules and cytokines in IRH patients.

Results Serum cytokines were elevated in various degrees in observation group. Compared with the control group, the cytokines levels were significantly higher (P < 0.05). After treatement with immunosuppressive drugs, the serum levels of cytokines in observation group reduced to a level close to the control group. HLADR were upregulated in activated tissue basophils, eosinophils, dendritic cells (DC) and macrophages of bone marrow in IRH patients, and POX activity in these immunocytes of IRH was higher than that of the control group. Immune molecules were highly expressed in eosinophils, DC and macrophages.

Conclusions It is demonstrated that antibodies or self-reactive lymphocytes were produced in IRH marrow, which would cause lesions of hemocytes, and lead to pathological process finally. Structure of hematopoietic cells mutated and these cells might be acted as target cells of immunocytes in the pathological process. Immunocytes could secrete inflammatory factors and lead to immunologic injury of hemocyte.

1 Szalay B, Acs L, Vásárhelyi B, Kovács L, Balog A. Successful use of tocilizumab in a patient with rheumatoid arthritis following severe pancytopenia during etanercept therapy. J Clin Rheumatol 2011;17(7):377-379.

2 Padigel UM, Lee JJ, Nolan TJ, Schad GA, Abraham D. Eosinophils can function as antigen-presenting cells to induce primary and secondary immune responses to Strongyloides stercoralis. Infect Immun 2006;74:3232-3238.

3 Letimier FA, Passini N, Gasparian S, Bianchi E, Rogge L. Chromatin remodeling by the SWI/SNF-like BAF complex and STAT 4 activation synergistically induce IL-12Rbeta2 expression during human Th1 cell differentiation. The EMBO J 2007;26:1292-1302. Bettelli E, Korn T, Oukka M, Kuchroo

4 VK. Iuduction and effector functionof T(H) 17 cells . Nature 2008;453(7198):1051-1057.

5 Oliveira LC, Porta G, Marin ML, Bittencourt PL, Kalil J, Goldberg AC. Autoimmune hepatitis, HLA and extended haplotypes. Autoimmun Rev 2011;10(4):189-193.

6 Sheehan NJ. HLA-B27: what’s new? Rheumatology (Oxford) 2010;49(4):621-631.

7 Colbert RA. The immunobiology of HLA-B27: variations on a theme. Curr Mol Med 2004;4:21-30.

8 Allen RL, Trowsdale J. Recognition of classical and heavy chain form of HLA-B27 by leukocyte receptor. Curr Mol Med 2004; 4(1):59-65.

9 Deng J, Younge BR, Olshen RA, Goronzy JJ, Weyand CM. Th17 and Th1 T-cell responses in giant cell arteritis. Circulation 2010;121:906-915.

10 Ekinci NS, Alpsoy E, Karakas AA, Yilmaz SB, Yegin O. IL-17A has an important role in the acute attacks of Behçet’s disease. J Invest Dermatol 2010;130(8):2136-2138.

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