Introduction: Quince (Cydonia oblonga Mill.) is a plant of which both the fruits and the leaves are sources of compounds with antioxidant potential. Such activity could be helpful to prevent the development of so-called oxidative stress.
Objective: The aim of the study was to evaluate the antioxidant properties of ethanolic, methanolic and acetonic extracts of mature and immature quince fruits, as well as leaves.
Methods: The extracts were prepared using ultrasound-assisted extraction, for 15, 30 and 60 minutes. The antioxidant activity was assessed by DPPH, FRAP, ABTS and Folin-Ciocalteu (F-C) methods.
Results: Antioxidant activity of all of the evaluated extracts were observed. The highest potential determined with each method was found for leaf extracts. Moreover, higher activity of unripe fruit extracts compared to ripe fruit was observed. Taking into account the applied extractants, the highest antioxidant capacity was found for methanolic extracts, extracted for 60 and 30 minutes. By contrast, the lowest potential was observed mainly for ethanolic extracts (extraction time 15 minutes).
Conclusion: Quince extracts, particularly alcoholic extracts of leaves, seem to be a valuable source of anti-oxidants. Factors as extraction time, the type of solvent and degree of fruit maturity may influence the antioxidant activity of extracts.
1. Thanan R, Oikawa S, Hiraku Y, Ohnishi S, Ma N, Pinlaor S et al. Oxidative stress and its significant roles in neurodegenerative diseases and cancer. Int J Mol Med Sci 2014; 16(1):193-217. doi: http://dx.doi.org/10.3390/ijms16010193
6. Stojanović BT, Mitić SS, Stojanović GS, Mitić MN, Kostić DA, Paunović DD et al. Phenolic profiles and metal ions analyses of pulp and peel of fruits and seeds of quince (Cydonia oblonga Mill.). Food Chem 2017; 232:466-75. doi: http://dx.doi.org/10.1016/j.foodchem.2017.04.041
9. Muzykiewicz A, Zielonka-Brzezicka J, Klimowicz A, Florkowska K. Rowan (Sorbus aucuparia L.) as a source of compounds with potential anti-oxidant properties –comparison of antioxidant properties of leaves, flowers and fruit extracts. Probl Hig Epidemiol 2017; 98:125-32.
10. Zielonka-Brzezicka J, Nowak A, Zielińska M, Klimowicz A. Comparison of the antioxidant properties of selected parts of raspberry (Rubus idaeus) and blackberry (Rubus fruticosus). Pomeranian J Life Sci 2016; 62(4):52-9. doi: http://dx.doi.org/10.21164/pomjlifesci.269
11. Nowak A, Zielonka-Brzezicka J, Pechaiko D, Tkacz M, Klimowicz A. The evaluation of the antioxidant properties of Ginkgo biloba L. leaves after the end of the growing season. Pomeranian J Life Sci 2017; 63(1):24-30. doi: http://dx.doi.org/10.21164/pomjlifesci.222
12. Costa RM, Magalhães AS, Pereira JA, Andrade PB, Valentão P, Carvalho M et al. Evaluation of free radical-scavenging and antihemolytic activities of quince (Cydonia oblonga) leaf: a comparative study with green tea (Camellia sinensis). Food Chem Toxicol 2009; 47:860-65. doi: http://dx.doi.org/10.1016/j.fct.2009.01.019
14. Matysiak M, Gaweł-Bęben K, Rybczyńska K, Gmiński J, Surma S. Comparing selected biological properties of garlic (Allium sativum L.) from Poland and China. Żywn Nauka Technol Jakość 2015; 2(99):160-9. doi: http://dx.doi.org/10.15193/zntj/2015/99/030
15. Apak R, Özyürek M, Güçlü K, Çapanoğlu E. Antioxidant activity/capacity measurement. 1. Classification, physicochemical principles, mechanisms, and electron transfer (ET)-based assays. J Agric Food Chem 2016; 64:997-1027. doi: http://dx.doi.org/10.1021/acs.jafc.5b04739
16. Wojdyło A, Oszmiański J, Bielicki P. Polyphenolic composition, antioxidant activity, and polyphenol oxidase (PPO) activity of quince (Cydonia oblonga Miller) varieties. J Agric Food Chem 2013; 61:2762-72. doi: http://dx.doi.org/10.1021/jf304969b
17. Hamauzu Y, Yasui H, Inno T, Kume C, Omanyuda M. Phenolic profile, antioxidant property, and anti-influenza viral activity of Chinese quince (Pseudocydonia sinensis Schneid.), quince (Cydonia oblonga Mill.), and apple (Malus domestica Mill.) fruits. J Agric Food Chem 2005; 53:928-34. doi: http://dx.doi.org/10.1021/jf0494635
20. Sati P, Pandey A, Rawat S, Rani A. Phytochemicals and antioxidants in leaf extracts of Ginkgo biloba with reference to location, seasonal variation and solvent system. J Pharm Res 2013; 7:804-9. doi: http://dx.doi.org/10.1016/j.jopr.2013.09.001
21. Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S et al. Effect of extrac-Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal 2014; 22:296-302. doi: http://dx.doi.org/10.1016/j.jfda.2013.11.001
22. Ghitescu RE, Volf I, Carausu C, Bühlmann AM, Gilca IA, Popa VI. Optimization of ultra-ultra-sound-assisted extraction of polyphenols from spruce wood bark. Ultrason Sonochem 2015; 22:535-41. doi: http://dx.doi.org/10.1016/j.ultsonch.2014.07.013
23. Bimakr M, Rahman RA, Taip FS, Adzahan NM, Sarker MZ, Ganjloo A. Optimization of ultrasound-assisted extraction of crude oil from winter melon (Benincasa hispida) seed using response surface methodology and evaluation of its antioxidant activity, total phenolic content and fatty acid composition. Molecules 2012; 17(10):11748-62. doi: http://dx.doi.org/10.3390/molecules171011748