Determination of lotaustralin in Rhodiola species

Open access


In our research, the concentration of lotaustralin in the roots of two species Rhodiola kirilowii and Rhodiola rosea were compared. Aqueous and hydroalcoholic extracts from those plants were analyzed too. To determine the content of this compound the ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. The obtained results showed that the content of measured lotaustralin depends on the species of Rhodiola. R. rosea roots are the richer source of lotaustralin then R. kirilowii. The same situation was observed in the extracts. A hydroalcoholic extract from R. rosea contains up to 135.276 mg of lotaustralin in 100 g of dry powdered material. In the case of R. kirilowii extracts, an aqueous extract contained more lotaustralin (74.791 mg/100 g of dry powdered material) then a hydroalcoholic extract.

1. Siegien I. Cyanogenesis in plants and its role in herbivore defense. Kosmos. Problemy Nauk Biologicznych 2007; 56 1-2 (274-275):155-166.

2. Seigler DS. Cyanogenic glycosides and cyanolipids. [In:] Seigler DS. Plant Secondary Metabolism. Kluwer Academic Press, Boston 1998; 273-296.

3. Yip W-K, Yang SF. Cyanide metabolism in relation to ethylene production in plant tissues. Plant Physiol 1988; 88:473-476.

4. Zilg H, Conn EE. Stereochemical aspects of lotaustralin biosynthesis. J Biol Chem 1974; 249(10):3112-3115.

5. Wolski T, Baj T, Ludwiczuk A, Głowniak K, Czarnecka G. Rhodiola genus taxonomy chemical composition, activity and use also phyochemical analysis of roots of two species of Rhodiola: Rhodiola rosea L. and Rhodiola quadrifida (Pall.) Fish et Mey. Postępy Fitoterapii 2008; 11(1):2-14.

6. Gryszczyńska A, Mielcarek S, Buchwald W. The determination of flavan-3-ol content in the root of Rhodiola Kirilowii. Herba Pol 2011; 51(1): 27-37.

7. Yousef GG , Grace MH, Cheng DM, Belolipov IV, Raskin I, Lila MA. Comparative phytochemical characterization of three Rhodiola species. Phytochemistry 2006; 67:2380-2391.

8. Assessment report on Rhodiola rosea L., rhizoma et radix. Committee on Herbal Medicinal Products (HMPC), EMA/HMPC/232100/2011, 27 March 2012.

9. Panossian A, Wikmana G, Sarris J. Rosenroot (Rhodiola rosea): Traditional use, chemical composition, pharmacology and clinical efficacy. Phytomedicine 2010; 17(7):481-493.

10. Wiedenfeld H, Zych M, Buchwald W, Furmanow1a M. New compounds from Rhodiola kirilowii. Sci Pharm 2007;75:29-34.

11. Krajewska-Patan A, Furmanowa A, Mścisz A, Dreger M, Łowicka A, Górska-Paukszta M, et al. Tissue culture of Rhodiola Kirilowii (Regel.) Maxim - contents of biologically active compounds at different stages of growth. Herba Pol 2006; 52(4):98-106.

12. Kang S, Wang J, Zhang J, Liu FY, Xu Z. The isolation and identification of p-hydroxyphenrthyl B-dglycoside (salidroside) and lotaustralin in 10 species of Rhodiola using GC are reported. Chin Mater Med 1998; 23:365-366.

13. Kang S, Zhang J, Lu Y, Lu D. Chemical constituents of Rhodiola Kirilowii (Reg.) Reg. Chung Kuo Chung Yao Tsa Chih 1992; 17:100-101.

14. Peng N, Ma CY, Ge YC. Chemical constituents of Rhodiola Kirilowii Regel. Zhongguo Zhong Yao Za Zhi 1994; 19(11):676-7, 702.

15. Zhang S, Wang J, Zhang H. Chemical costituents of Tibetan medicinal herb Rhodiola Kirilowii (Reg.) Reg Zhongguo Zhong Yao Za Zhi 1991; 16(8):483-512.

16. Kang S, Wang J. Comaprative study of the constituents from 10 Rhodiola plants. Zhong Yao Cai 1997; 20(12):616-8.

17. Rojas MG, Morales-Ramo JA. Tri-trophic level impact of host plant linamarin and lotaustralin on Tetranychus urticae and its predator Phytoseiulus persimilis. J Chem Ecol 2010; 36:1354-1362.

18. Nyirenda DB, Chiwona-Karltun L, Chitundu M, Haggblade S, Brimer L. Chemical safety of cassava products in regions adopting cassava production and processing - Experience from Southern Africa, Food Chem Toxic 2011; 49:607-612.

19. Burns AE, Howard Bradbury J, Cavagnaro TR, Gleadow RM. Total cyanide content of cassava food products in Australia. J Food Comp Anal 2011; 25:79-82.

20. Akgul Y, Ferreira D, Abourashed EA, Khan IA. Lotaustralin from Rhodiola rosea roots. Phytochemical Communications. Fitoterapia 2004; 75:612-614.

21. Xiao WQR. Quantitative Analysis of Salidroside, Tyrosol and Lotaustralin in Roots of Rhodiola L. Plants Grown at Tianshan Mountain. Chinese J Pharmaceut Anal 2005; 09:1026-1029.

22. Stochmal A, Oleszek W. Changes of cyanogenic glucosides in white clover (trifolium repens l.) during the growing season. J Agric Food Chem 1997; 45:4333-4336.

23. Busk PK, Moller BL. Dhurrin synthesis in sorghum is regulated at the transcriptional level and induced by nitrogen fertilization in older plants. Plant Physiol 2002; 129(3):1222-31.

24. Vetter J. Plant cyanogenic glycosides. Toxicon 2000; 38:11-36.

Herba Polonica

From Botanical to Medical Research

Journal Information


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 154 150 4
PDF Downloads 57 56 2