Determination of chlorogenic and gallic acids by UPLC-MS/MS

Open access


The aim of our study were qualitative and quantitative analyses of two polyphenolic acids: chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola: R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts were prepared. In order to identify analysed polyphenolic compounds ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic acid is commonly found in the roots of these plants. Aqueous extract in both species is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic method developed by our team is more precise then previously published.

1. Olszewska M. Quantitative HPLC analysis of flavonoids and chlorogenic acid in the leaves and inflorescences of Prunus Serotina Ehrh. Acta Chromatogr 2008; 19:253-269.

2. Belay A, Gholap AV. Characterization and determination of chlorogenic acids (CGA) in coffee beans by UV-Vis spectroscopy. Afr J Pure Appl Chem 2009; 3(11):234-240.

3. Yuan B, Qioa M, Xu H, Wang L, Li F. Determination of chlorogenic acid in rat plasma by high performance liquid chromatography after peritoneal administration of compound Daqingye injection. Yakugaku Zasshi 2006; 126(9):811-814.

4. Borde VU, Pangricar PP, Tecale SU. Gallic acid in Ayurvedic herbs and formulations. Rec Res Sci Tech 2011; 3(7):51-54.

5. Gryszczyńska A, Mielcarek S, Buchwald W. The determination of flavan-3-ol content in the root of Rhodiola Kirilowii. Herba Pol 2011; 51(1):27-37.

6. Cui S, Hu X, Chen X, Hu Z. Determination of p-thyrosol and salidroside in three samples of Rhodiola crenulata and one of Rhodiola Kirilowii by capillary ion electrophoresis. Anal Bioanal Chem 2003; 377:370-374.

7. Wiedenfeld H, Zych M, Buchwald W, Furmanowa M. New compounds from Rhodiola Kirilowii. Sci Pharm 2007; 75:29-34.

8. Krajewska-Patan A, Furmanowa A, Mścisz A, Dreger M, Łowicka A, Górska-Paukszta M et al. Tissue culture of Rhodiola Kirilowii (Regel.) Maxim - contents of biologically active compounds at different stages of growth. Herba Pol 2006; 52(4):98-106.

9. Kang S, Wang J, Zhang J, Liu FY, Xu Z. Quantitative analysis of salidroside and lotaustralin in Rhodiola by gas chromatography. Chin Mater Med 1998; 23:365-366.

10. Kang S, Zhang J, Lu Y, Lu D. Chemical constituents of Rhodiola Kirilowii (Reg.). Chung Kuo Chung Yao Tsa Chih 1992; 17:100-101.

11. Pengj N, Ma CY, Ge YC. Chemical constituents of Rhodiola Kirilowii Regel. Zhongguo Zhong Yao Za Zhi 1994; 19(11):676-702.

12. Zhang S, Wang J, Zhang H. Chemical costituents of Tibetan medicinal herb Rhodiola Kirilowii (Reg.) Reg. Zhongguo Zhong Yao Za Zhi 1991; 16(8):483-512.

13. Panossian A, Wilkman G, Sarris J. Rosenroot (Rhodiola rosea): tradicional use, chemical composition, pharmacology and clinical efficacy. Phytomedicine 2010; 17:481-493.

14. Khanum F, Bawa AS, Singh B. Rhodiola rosea: a versatile adaptogen. Comp Rev Food Sci Food Safety 2005; 4:55-62 .

15. Saratikov AS, Krasnov EA, Khnikina LA, Duvidson LM. Isolation and chemical analysis of individual biologically active constituents of Rhodiola rosea. Proc Siberian Acad Sci Biol 1967; 1:54-60.

16. Kurkin VA, Zapesochnaya GG. Chemical composition and pharmacological characteristics of Rhodiola rosea [review]. J Med Plants (Moscow) 1985; 1231-445.

17. Lee MW, Lee YA, Park HM, Roh SH, Lee EJ, Jang HD, Kin YH. Antioxidative phenolic compounds from the roots of Rhodiola sachalinensis A. Bor Arch Pharm Res 2000; 23:455-8.

18. Ohsugi M, Fan W, Hase K, Xiang Q, Tezuka Y, Komatsu K et al. Active-oxygen scavenging activity of traditional nourishing - tonic herbal medicines and active constituents of Rhodiola sacra. J Ethnopharmacol 1999; 67:111-9.

Herba Polonica

From Botanical to Medical Research

Journal Information


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 219 187 19
PDF Downloads 163 157 24