Cathaemasia hians infection in Black stork in Slovakia: morphological and histopathological study

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Summary

Cathaemasia hians is an obligate trematode parasite of Black storks that are on the List of protected birds in Europe. In the present study, adult trematodes were isolated from the Black stork post mortem and morphological study revealed C. hians species. In total, 10 worms were found in the oesophagus and the ventriculus of the bird. Histopathological examination of the tissue samples of oesophagus, proventriculus and ventriculus was performed on paraffin sections using a set of staining procedures. The sporadic lesions were seen in the tela submucosa of oesophagus containing connective tissue mast cells, eosinophils and heterophils and some foci were surrounded by the fibrous tissue. In addition, a few inflammatory nodules had larval-like material inside, probably being of the same species. There were no visible morphological alterations in the epithelial layer of lamina propria mucosae of proventriculus, rich in goblet cells as well as in the tela submucosa. Majority of trematodes were localized in the ventriculus, where the lamina propria mucosae was damaged or disrupted sporadically. In these sites, in the tela submucosa, a various food-originated inorganic/organic material and eggs of C. hians were deposited, stimulating a weak inflammatory response. Nodules containing larvae were not observed in any of ventriculus tissue layers. This study demonstrated, for the first time, infection with adults of C. hians trematode in the Black stork nesting in Slovakia. The presence of larvae and eggs in the tissues of the upper gastrointestinal tract of bird was associated with mild inflammatory response but feeding behaviour of adult worms in the ventriculus probably contributed to the enhanced susceptibility of the lamina propria mucosae to mechanical damage by inorganic material. Although larval stages have not yet been documented in the intermediate hosts in Slovakia, our report indicates that the life cycle of C. hians might occur in Central Europe.

ALONSO, J.C., ALONSO, J.A., CARRASCAL, L.M. (1991): Habitat selection by foraging white storks, Ciconia ciconia, during the breeding season. Can. J. Zoology, 69: 1957 - 1962. DOI: 10.1139/z91-270

CARRASCAL, L.M., BAUTISTA, L.M., LÁZARO, E. (1993): Geographical variation in the density of the white stork Ciconia ciconia in Spain: infl uence of habitat structure and climate. Biol. Conserv., 65: 83 - 87

DING, B.A., PIRONE, A., LENZI, C., XIAOMING, N., BAGLINI, A., ROMBOLI, I. (2011). Histochemical features of the Muscovy duck small intestine during development. Tissue Cell, 43: 190 - 195. DOI:10.1016/j. tice.2011.03.001

EDELÉNYI, B. (1974): Trematodes II., Fauna Hungariae 117. Akadémiai Kiado, Budapest, 224

ENERBACK, L. (1966). Mast cells in rat gastrointestinal mucosa. I. Effects of fi xation. Acta Pathol. Microbiol. Scand., 66: 289 - 302.

FALTÝNKOVÁ, A., NAŠINCOVÁ, V., KABLÁSKOVÁ, L. (2008): Larval trematodes (Digenea) of planorbid snails (Gastropoda: Pulmonata) in central Europe: A survey of species and key to their identifi cation. Syst. Parasitol., 69: 155 - 178. DOI: 10.1007/s11230-007-9127-1

GRABDA-KAZUBSKA, B., BAYSSADE-DUFOUR, C., KISELIENE, V. (1990): Chaemotaxy and excretory system of Echinocercaria choanophila U. Szidat, 1936, a larval form of Cathaemasia hians (Rud., 1809) (Trematoda, Cathemasiidae). Acta Parasitol. Pol., 35: 97 - 105

HENZ, B.M., MAURER, M., LIPPERT, U., WORM, M., BABINA, M. (2001): Mast cells as initiators of immunity and host defense. Exp. Dermatol., 10: 1 - 10. DOI: 10.1034/j.1600-0625.2001.100101.x

HÖFLE, U., KRONE, O., BLANCO, J.M., PIZZARO, M. (2003): Chaunocephalus ferox in free-living white storks in Central Spain. Avian Dis., 47: 506 - 512. DOI: 10.1637/0005-2086(2003)047[0506:CFIFWS]2.0. CO:2

HRČKOVÁ, G., VELEBNÝ, S., SOLÁR, P. (2010): Dynamics of hepatic stellate cells, collagen types I and III synthesis and gene expression of selected cytokines during hepatic fi brogenesis following Mesocestoides vogae (Cestoda) infection in mice. Int. J. Parasitol., 40(2): 163 - 174. DOI: 10.1016/j.ijpara.2009.06.008

HURNÍKOVÁ, Z., HRČKOVÁ, G., AGREN, E., KOMOROVÁ, P., FORSMAN, J., CHOVANOVÁ, B., MOLNÁR, L., LETKOVÁ, V. (2014). First fi nding of Trichinella pseudospiralis in two Tawny Owls (Strix aluco) from Sweden. Helminthologia, 50(3): 190 - 197. DOI: 10.2478/s11687-014-0228-5

CHERNOGORENKO, M.I. (1983): Larval trematodes in molluscs of the Dnieper River and its reservoirs. Kyiv: Nauk dumka, 212

KARASKA, D. (2002): Bocian čierny (Ciconia nigra). In: Danko, Š., Darolová, A., Krištín, A. (Eds) Rozšírenie vtákov na Slovensku. Bratislava, Slovakia: Veda, pp. 688

KÖNIGOVÁ, A., MOLNÁR, L., HRČKOVÁ, G., VÁRADY, M. (2013). The fi rst report of serraspiculiasis in Great Tit (Parus major) in Slovakia. Helminthologia, 50(4): 254 - 260. DOI: 10.2478/s11687-013-0138-y

KOSTADINOVA, A. (2005): Family Cathaemasiidae Fuhrmann, 1928. In: JONES, A., BRAY, R.A., GIBSON, D.I. (Eds) Keys to the Trematoda. Volume 2. London, UK: CABI Publishing and The Natural History Museum, pp. 69 - 73

LIPTOVSZKY, M., MAJOROS, G., PERGE, E. (2012): Cathaemasia hians in a Black Stork (Ciconia nigra) in Hungary. J. Wildl. Dis., 48(3): 809 - 811. DOI: 10.7589/0090-3558-48.3.809

MACKO, J.K. (1960): On the fauna of plathelminthes of Black stork - Ciconia nigra. L. Biologia, 7: 549 - 552

MERINO, S., MARTINEZ, J., LANZAROT, P., CANO, L.S., FERNANDEZ-GARCIA, M., RODRIGUEZ-CAABEIRO, F. (2001): Cathaemasia hians (Trematoda: Cathaemasiidae) infecting Black Stork nestlings (Ciconia nigra) from central Spain. Avian Pathol., 30: 559 - 561. DOI: 10.1080/03079450120078761

MEYERHOLZ, D.K., GRIFFIN, M.A., CASTILOW, E.M., VARGA, S.M. (2009). Comparison of histochemical methods for murine eosinophil detection in an RSV vaccine-enhanced infl ammation model. Toxicol. Pathology, 37: 249 - 255. DOI: 10.1177/0192623308329342

OKULEWICZ, A., SITKO, J. (2012): Parasitic helminthes - probable cause of death of birds. Helminthologia, 49(4): 241 - 246. DOI: 10.2478/s11687-012-0045-7

REEVES, H.L., FRIEDMAN, S.L. (2002): Activation of hepatic stellate cells - a key issue in liver fi brosis. Front. Biosci., 7: d808 - 826

SANTORO, M., BEGLI UBERTI, B., GALIERO, G., DI PRISCO, F., ALESSIO, N.D., VENEZIANO, V. (2013): Chaunocephalus ferox (Digenea: Echinostomatidae) infection associated with fatal cachexia in a white stork (Ciconia ciconia). Helminthologia, 50(3): 181 - 184. DOI: 10.2478/s11687-013-0129-z

SEY, O. (1965): Studies on endoparasitic helminths of Hungary. Acta Acad. Paedag. Civ. Pecs, 9: 179 - 199

SCHUSTER, R., SCHAFFER, T., SHIMALOV, V. (2002): Die Helminthenfauna einhemisher Weisstőrche (Ciconia ciconia). Berl. Munch. Tierarztl. Wochenschr., 115: 435 - 439

SITKO, J., HENEBERG, P. (2015). Composition, structure and pattern of helminth assemblages associated with central European storks (Ciconiidae). Parasitol. Int., 64: 130 - 134. DOI: org/10.1016/j. parint.2014.11.004

TURK, D.E. (1982). Symposium: The avian gastrointestinal tract and digestion. The anatomy of the avian digestive tract as related to feed utilization. Poultry Sci., 61: 1225 - 1244

ZHYTOVA, O.P., KOROL, E.M. (2012): Cathaemasia hians (Digenea, Cathaemasiidae) from Planorbis planorbis (Mollusca, Gastropoda) in reservoirs of central Polissya. Vest. zool., 46(4): e-42 - e-45. DOI: 10.2478/v10058-012-0029-6

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