Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

Open access


The differences in protein and enzymatic profiles of infective larvae (L3) of Trichostrongylus colubriformis, both induced and non-induced to hypobiosis, have been evaluated by means of SDS-PAGE and densitometric analysis as well as by semiquantitative micromethod API-ZYM (Bio-Mérieux, France). Quantitative differences were identified in protein levels between the induced and non-induced larvae, where the amount of two polypeptides (200–220 kDa) decreased in range 32.3–35.4 % and the amount of six polypeptides (20–28 kDa) increased in range 20.0–27.0 % in the samples of induced larvae. In contrast to non-induced larvae, on gelatin-substrate gel in L3 in vitro released (IVR) proteases from larvae conditioned to hypobiosis, zones of proteolysis were observed between 21 and 34 kDa.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • [1] Almería S. LLorente M. M. Uriarte J. (1996): Monthly fluctuations of worm burdens and hypobiosis of gastrointestinal nematodes of calves in extensive management systems in the Pyrenees (Spain). Vet. Parasitol. 67: 225–236 http://dx.doi.org/10.1016/S0304-4017(96)01037-0

  • [2] Armour J. Jennings F. W. Urguhart G. M. (1969): Inhibition of Ostertagia ostertagi at the early fourth stage. The influence of environment on host or parasite. Res. Vet. Sci. 10: 238–244

  • [3] Armour J. Bruce R. G. (1974): Inhibited development in Ostertagia ostertagi infections — a diapause phenomenon in a nematode. Parasitology 69: 161–174

  • [4] Connan R. M. (1971): The Seasonal Incidence of Inhibition of Development in Haemonchus contortus. Res. Vet. Sci. 12: 272–274

  • [5] De Cock H. Knox D. P. Claerebout E. DE Graaf D. C. (1993): Partial characterisation of proteolytic enzymes in different developmental stages of Ostertagia ostertagi. J. Helminthol. 67: 271–278 http://dx.doi.org/10.1017/S0022149X00013262

  • [6] Dopchiz M. C. Parma A. E. Fiel C. A. (2000): Hypobiosis induction alters the protein profile of Ostertagia ostertagi (Nematoda: Trichostrongylidae) Folia Parasitol. 47: 135–140

  • [7] Eysker M. (1993): The role of inhibited development in the epidemiology of Ostertagia infections Vet. Parasitol. 46: 259–269 http://dx.doi.org/10.1016/0304-4017(93)90063-S

  • [8] Gamble H. R. Mansfield L. S. (1996): Characterisation of excretory — secretory products from larval stages of Haemonchus contortus. Vet. Parasitol. 62: 291–305 http://dx.doi.org/10.1016/0304-4017(95)00871-3

  • [9] Geldhof P. Claerebout E. Knox D. P. Agneessens J. Vercruysse J. (2000): Proteinases released in vitro by the parasitic stages of the bovinne abomasal nematode Ostertagia ostertagi. Parasitology 121: 639–647 http://dx.doi.org/10.1017/S0031182000006806

  • [10] Hadas E. Stankiewicz M. (1997): Proteolytic enzymes of infective larvae and adults of Trichostrongylus colubriformis and Haemonchus contortus. Parasitol. Res. 83: 47–51 http://dx.doi.org/10.1007/s004360050206

  • [11] Healer J. Ashall F. Maizels R. M. (1991): Characterisation of proteolytic enzymes from larval and adult Nippostrongylus brasiliensis. Parasitology 103: 305–314 http://dx.doi.org/10.1017/S0031182000059588

  • [12] Jankovská I. Langrová I. Vadlejch J. (2003a): Arrested development of Trichostrongylus colubriformis in experimentally infected rabbits. Effect of acquired resistance single multiple doses and size of doses on rise of hypobiosis. Scientia Agriculturae Bohemica 34: 58–62.

  • [13] Jankovská I. Langrová I. Fučíková A. Borovský M. (2003b): Effect of parasitic nematodes Trichostrongylus colubriformis on haematological parameters of model host (Oryctolagus cuniculus f. domestica). Scientia Agriculturae Bohemica 34: 94–98

  • [14] Kooyman F. N. J. Eysker M. (1995): Analysis of proteins related to conditions for arrested development and differentiation in Haemonchus contortus by two — dimmensional gel electrophoresis. Int. J. Parasitol. 25: 561–568 http://dx.doi.org/10.1016/0020-7519(94)00161-G

  • [15] Langrová I. Jankovská I. (2004): Arrested development of Trichostrongylus colubriformis in experimentally infected rabbits. Effect of decreasing photoperiod low temperature and desiccation. Helminthologia 41: 85–90

  • [16] McKerrow J. H. Brindley P. Brown M. Gam A. Staunton C. Neva A. F. (1990): Strongyloides stercoralis: Identification of a Protease that facilitates Penetration of Skin by the Infective Larva. Exp. Parasitol. 70: 134–143 http://dx.doi.org/10.1016/0014-4894(90)90094-S

  • [17] Tort J. Brindley P. J. Knox D. Wolfe K. H. Dalton J. P. (1999): Proteinases and associated genes of parasitic helminths. Adv. Parasit. 43: 161–166 http://dx.doi.org/10.1016/S0065-308X(08)60243-2

Journal information
Impact Factor

IMPACT FACTOR 2018: 0.731
5-year IMPACT FACTOR: 0.634

CiteScore 2018: 0.8

SCImago Journal Rank (SJR) 2018: 0.398
Source Normalized Impact per Paper (SNIP) 2018: 0.554

Target audience: researchers in the field of human, veterinary medicine and natural science
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 185 129 6
PDF Downloads 63 49 3