Identification of Plasmodiophora brassicae Wor. isolate suppressing clubroot resistance in ‘Kilaxy’ F1 white cabbage

Open access


The purpose of this study was the evaluation of the pathogenicity of Plasmodiophora brassicae Wor. field isolates gathered from clubroot-infected white cabbage Brassica oleracea var. capitata L. f. alba plants grown in different areas of Poland. The virulence of seven isolates of the pathogen was tested. Breeding materials from white cabbage resistant and susceptible to P. brassicae were used as standard objects for isolate virulence screening. Cabbage seeds were infected by submerging them into a resting-spore suspension. A screening of plant resistance using a nine-degree scale based on root symptoms was carried out at the eight-week-old plantlets stage and at harvest time on afield infected by P. brassicae spores. Isolates from Pobiednik and Grębałów were defined as more virulent. The P isolate from Pobiednik also infested plants of the clubroot resistant ‘Kilaxy’ F1 cultivar, which indicated the suppression of clubroot resistance. Its reaction to the other six isolates confirmed the resistance of this cultivar. The susceptible HTM line was characterised by the highest infestation level. A difference in the susceptibility level was noticed between lines derived from those with resistant genes, i.e. Oregon123 and ‘Badger Shipper’. The Oregon123 line was less susceptible to the applied isolates. The effect of the isolates used for seed inoculation on the reaction of the dormant plants to the pathogen on the clubroot field was observed.

Diederichsen E., Frauen M., Linders E., Hatakeyama K., Hirai M., 2009. Status and perspectives of clubroot resistance breeding in crucifer crops. J. Plant Growth Regul. 28: 265-281.

Dixon G.R., 2009a. The occurrence and economic impact of Plasmodiophora brassicae and clubroot diesase. J. Plant Growth Regul. 28: 194-202.

Dixon G.R., 2009b. Plasmodiophora brassicae in its environment. J. Plant Growth Regul. 28: 212-228.

Dixon G.R. 2012. Clubroot - control needs knowledge. The Vegetable Farmer T/A Greegene International 01/2012: 1-4.

Hirai M., 2006. Genetic analysis of clubroot resistance in Brassica crops. Breeding Sci. 56: 223-229.

Donald C., Porter I., 2009. Integrated control of clubroot. J. Plant Growth Regul. 28: 289-303.

Jędryczka M., Korbas M., Jajor E., Danielewicz J., Kaczmarek J., 2013. The occurrence of Plasmodiophora brassicae in agricultural soils in the Wielkopolska region, in 2011-2012. Prog. Plant Prot. 53(4): 774-778.

Jędryczka M., Wojciechowski A., Korbas M., Olej niczak J., Niem ann J., Jajor E., Brachaczek A., Kaczmarek J., Książczyk T., 2012. The incidence of clubroot on winter oilseed rape in Poland and the search for sources of genetic resistance in interspecific hybrids and mutants within the genus Brassica. Abstracs 31th Scientific Conference Oilseed Crops, Poznań, 49-51.

Kageyama K., Asano T., 2009. Life cycle of Plasmodiophora brassicae. J. Plant Growth Regul. 28: 203-211.

Laszczak P., Szczep onek A., Wesołowska M., Michalik B., 2006. Comparison of virulence of Polish Plasmodiophora brassicae pathotypes. Proc. I (IX) Conference of Young Botanists in Saint-Petersburg, May 21-26: 305.

Lewis S.J., Brokenshire T., 1978. A comparison of plant inoculation techniques for the determination of Plasmodiophora brassicae populations. Proc. Woronin +100 Conference, Madison: 54-67.

Manzanares-Dauleux M.J., Divaret I., Baron F., Thomas G., 2000. Evaluation of French Brassica oleracea landraces for resistance to Plasmodiophora brassicae. Euphytica 113: 211-218.

Osaki K., Fujiyama S., Nakayama A., Shimizu Y., Ito S., Tanaka S., 2008. Relation between pathogenicity and genetic variation within Plasmodiophora brassicae. J. Gen. Plant Pathol. 74: 281-288.

Piao Z., Ramchiary N., Lim Y.P., 2009. Genetic of clubroot resistance in Brassica species. J. Plant Growth Regul. 28: 252-264.

Reby E., Michalik B., 2005. Ocena podatności odmian wzorcowych kapusty z genami odporności na krajowe izolaty kiły (Plasmodiophora brassicae) In: Michalik B., Żurawicz E. (Eds). Zmienność genetyczna i jej wykorzystanie w hodowli roślin ogrodniczych, ISiK, Skierniewice: 37-42.

Robak J., 1991. Zmienność patotypów Plasmodiophora brassicae Wor. występujących w Polsce i ich patogeniczność w stosunku do odmian i linii hodowlanych Brassica oleracea. Praca habilitacyjna. Instytut Warzywnictwa w Skierniewicach.

Robak J., Czubatka A., Czajka A., 2013. Kiła kapusty - monitorowanie i diagnostyka molekularna Plasmodiophora brassicae w uprawach roślin kapustowatych. Instytut Ogrodnictwa, Skierniewice: 1-13.

Wesołowska M., 2011. Isolate pathogenicity recognition of Plasmodiophora brassicae Wor. in different areas of Poland. Folia Hort. 23(2): 165-174.

Williams P. H., 1985. CrGC Resource Book Dept. of Plant Pathology University of Wisconsin, Madison.

Folia Horticulturae

The Journal of Polish Society for Horticultural Sciences (PSHS)

Journal Information

Web of Science, IMPACT FACTOR 2018: 0,532

CiteScore 2018: 0.6

SCImago Journal Rank (SJR) 2018: 0.198
Source Normalized Impact per Paper (SNIP) 2018: 0.644


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 218 162 17
PDF Downloads 100 82 9