Objectives. The deformability of erythrocytes is their ability to change shape in order to pass through the capillaries. Th is is necessary for quality of microcirculation and sufficient delivery of oxygen to the tissues. Th e aim of our study was to investigate the possible spontaneous changes in the erythrocyte deformability during day and evaluation of the possible direct effects of melatonin (hormone involved in regulation of biorhythms) on the erythrocyte deformability. Methods. Samples of capillary blood were taken from 12 healthy volunteers in the morning (8:00) and early in the evening (16:30). Determination of erythrocyte deformability was done based on the measurement of their filtrability. It was measured immediately aft er the sample collection and 2-hour lasting incubation without or with melatonin (2000 μmol/L). Results. Erythrocyte deformability was significantly lower in the morning (filtrability index: 0.68±0.01 morning vs. 0.71±0.01 early evening, p<0.05). Th e incubation of blood samples with melatonin did not have impact on deformability. Conclusions. We suggest the presence of diurnal changes in erythrocyte deformability with worse values in the morning that may contribute to higher risk of ischemic attacks in the morning hours. Direct in vitro effect of melatonin on deformability was not observed, but possible in vivo effects cannot be excluded.
If the inline PDF is not rendering correctly, you can download the PDF file here.
Berker M Dikmenoglu N Bozkurt G Ergonul Z Ozgen T. Hemorheology melatonin and pinealectomy. What’s the relationship? An experimental study. Clin Hemorheol Microcirc 30 47-52 2004
Benitez-King G. Melatonin as a cytoskeletal modulator: implications for cell physiology and disease. J Pineal Res 40 1-9 2006.
Chakravarty S Rizvi SI. Circadian modulation of sodium-potassium ATPase and sodium - proton exchanger in human erythrocytes: in vitro eff ect of melatonin. Cell Mol Bio 57 80-86 2011.
Csernus V Mess B. Biorhythms and pineal gland. Neuro Endocrinol Lett 24 404-411 2003.
Dikmenoglu N Ileri E Seringec N Ercil D. Melatonin prevents lipid peroxidation in human erythrocytes but augments deterioration of deformability aft er in vitro oxidative stress. Clin Hemorheol Microcirc 40 235-242 2008.
Gumustekin M Tekmen I Guneli E Tugyan K Topcu A Ergonen AT Ozdemir MH Uysal N Bediz CS. Short-term melatonin treatment improved diabetic nephropathy but did not aff ect hemorheological changes in diabetic rats. Pharmazie 62 693-698 2007.
Herichova I. Changes of physiological functions induced by shift work. Endocr Regul 47 159-170 2013.
Muller JE Ludmer PL Willich SN Tofl er GH Aylmer G Klangos I Stone PH. Circadian variation in the frequency of sudden cardiac death. Circulation 75 131-138 1987.
Radosinska J Vrbjar N. Th e role of red blood cell deformability and NaK-ATPase function in selected risk factors of cardiovascular diseases in humans: focus on hypertension diabetes mellitus and hypercholesterolemia. Physiol Res 65 Suppl 1 S43−S54 2016.
Radosinska J Mezesova L Okruhlicova L Frimmel K Breierova E Bartekova M Vrbjar N. Eff ect of yeast biomass with high content of carotenoids on erythrocyte deformability NO production and NaK-ATPase activity in healthy and LPS treated rats. Clin Hemorheol Microcirc 64 125-134 2016.
Raj K Bhatia R Prasad K Srivastava MV Vishnubhatla S Singh MB. Seasonal diff erences and circadian variation in stroke occurrence and stroke subtypes. J Stroke Cerebrovasc Dis 24 10-16 2015.
Schmid-Schonbein H. Microrheology of erythrocytes blood viscosity and the distribution of blood fl ow in the microcirculation. Int Rev Physiol 9 1-62 1976.
Segura D Eblen-Zajjur A Proverbio F Proverbio T Carrera F Caruso-Neves C Marin R. A blood plasma inhibitor is responsible for circadian changes in rat renal NaK-ATPase activity. Int J Biochem Cell Biol 36 2054-2065 2004.
Yerer MB Aydogan S. Th e importance of circadian rhythm alterations in erythrocyte deformability. Clin Hemorheol Microcirc 35 143-147 2006.