A laboratory experiment was completed to determine the effect of the herbicides Alister Grande 190 OD, Fuego 500 SC and Lumax 537.5 SE on counts of actinomycetes as well as the activity of enzymes and their resistance to herbicides. Sandy loam was mixed with appropriate doses of the herbicides, such as: 0 - the control, 1 - technological dose and doses 20-, 40-, 80- and 160-fold higher than recommended. On day 20, 40, 80 and 160, counts of actinomycetes and activity of urease, acid phosphatase and alkaline phosphatase were determined. For 160 days, soil was incubated at 25°C and its moisture content was maintained on a constant level equal 50% of water capillary capacity. On days 20 and 80 of the experiment, the ecophysiological (EP) and colony development (CD) indices were computed. Additionally, the resistance (RS) of enzymes to the herbicides was assessed on day 20 and their resilience index (RL) was determined on day 160. It has been found out that soil contamination with herbicides contributed to elevated counts of actinomycetes. The highest number of these microorganisms was observed in soil with Lumax 537.5 SE, and the lowest one appeared in soil with Alister Grande 190 OD. The CD for actinomycetes was the highest in treatments with Fuego 500 SC and the highest EP was determined in soil with Alister Grande 190 OD. Application of the herbicides in doses from 20- to 160-fold higher than recommended by the manufacturer significantly increased the activity of acid and alkaline phosphatases. With respect to the activity of urease, the herbicides produced variable effects. The strongest inhibitory effect on the activity of urease was produced by Fuego 500 SC, which reduced the activity of this enzyme by 13.39% when added to soil in a dose exceeding by 160-fold the recommended rate. The RS of the enzymes to the herbicides ranged from 0.461 to 0.955. Urease was the most tolerant to soil contamination with the herbicides.
 Tao L, Yang H. Fluroxypyr biodegradation in soils by multiple factors. Environ Monit Assess. 2011;175:227-238. DOI: 10.1007/s10661-010-1508-2.
 Zhang HB, Luo YM, Zhao QG, Wong MH, Zhang GL. Residues of organochlorine pesticides in Hong Kong soils. Chemosphere. 2006;63:633-641. DOI: 10.1016/j.chemosphere.2005.08.006.
 Emmerling C, Liebner C, Haubold-Rosar M, Katzur J, Schvoder D. Impact of application of organic west materials of microbial and enzyme activities of nine soil in the lusetion coal mining region. Plant Soil. 2000;220:129-138. DOI: 10.1023/A:1004784525209.
 Frączek K, Kozdrój J. Assessment of airborne actinomycetes in subterranean and earth sanatoriums. Ecol Chem Eng S. 2013;20(1):151-161. DOI: 10.2478/eces-2013-0012.
 Slavίk R, Julinová M, Labudίková M. Screening of the spatial distribution of risk metals in topsoil from an industrial complex. Ecol Chem Eng S. 2012;19(2):259-272. DOI: 10.2478/v10216-011-0020-0.
 Baćmaga M, Boros E, Kucharski J, Wyszkowska J. Enzymatic activity in soil contaminated with the Aurora 40 WG herbicide. Environ Protec Eng. 2012;38(1):91-102.
 Rahmansyah M, Antonius S, Sulistinah N. Phosphatase and urease instability caused by pesticides present in soil improved by grounded rice straw. J Agric Biol Sci. 2009;4(2):56-62.
 Singh DK, Kumar S. Nitrate reductase, arginine deaminase, urease and dehydrogenase activities in natural soil (ridges with forest) and in cotton soil after acetamiprid treatments. Chemosphere. 2008;71:412-418. DOI: 10.1016/j.chemosphere.2007.11.005.
 Carter MR. Soil Sampling and Methods of Analysis. London: Canadian Soc Soil Sci, Lewis Publishers; 1993.
 Nelson DW, Sommers LE. Total carbon, organic carbon, and organic matter. In: Method of Soil Analysis: Chemical Methods. Sparks DL, editor. Madison, WI: Amer Soc Agronomy; 1996; 1201-1229.
 Parkinson D, Gray FRG, Williams ST. Methods of Studying Ecology of Soil Microorganism. IBP Handbook. Oxford and Edinburgh: Blackwell Scientific Publications; 1997; 19.
 De Leij FAAM, Whipps JM, Lynch JM. The use of colony development for the characterization of bacterial communities in soil and on roots. Microb Ecol. 1993;27:81-97.
 Sarathchandra SU, Burch G, Cox NR. Growth patterns of bacterial communites in the rhizoplane and rhizosphere of with clover (Trifolium repens L.) and perennial ryegrass (Lolium perenne L.) in long-term pasture. Appl Soil Ecol. 1997;6:293-299.
 Mukherjee P, Alam S, Sardar D, Pahari A, Roy S, Chowdhury A. Persistence and dissipation of linuron (Afalon-50 WP) in pea cropped soil and its effect on soil microorganisms. Bull Environ Contam Toxicol. 2006;76(3):407-414. DOI: 10.1007/s00128-006-0936-8.
 Araújo ASF, Monteiro RTR, Abarkeli RB. Effect of glyphosate on the microbial activity of two Brazilian soil. Chemosphere. 2003;52:799-804. DOI: 10.1016/S0045-6535(03)00266-2.
 Crouzet O, Batisson I, Besse-Hoggan P, Bonnemoy F, Bardot C, Poly F, et al. Response of soil microbial communities to the herbicide mesotrione: A dose-effect microcosm approach. Soil Biol Biochem. 2010;42:193-202. DOI: 10.1016/j.soilbio.2009.10.016.
 Kucharski J, Wyszkowska J. Biological properties of soil contaminated with the herbicyde Apyros 75 WG. J Elem. 2008;13(3):357-371.
 Ratcliff AW, Busse MD, Shestak CJ. Changes in microbial community structure following herbicide (glyphosate) addition to forest soils. Appl Soil Ecol. 2006;34:114-124. DOI: 10.1016/j.apsoil.2006.03.002.
 Cycoń M, Piotrowska-Seget Z. Changes in bacterial diversity and community structure following pesticides addition to soil estimated by cultivation technique. Ecotoxicology. 2009;18(5):632-642. DOI: 10.1007/s10646-009-0321-6.
 Cycoń M, Piotrowska-Seget Z, Kozdrój J. Linuron effects on microbiological characteristics of sandy soils as determined in a pot study. Ann Microbiol. 2010;60(3):439-449. DOI: 10.1007/s13213-010-0061-0.
 Ros M, Goberna M, Moreno JL, Hernandez T, Garcı′a C, Insam H, et al. Molecular and physiological bacterial diversity of a semi-arid soil contaminated with different levels of formulated atrazine. Appl Soil Ecol. 2006;34:93-102. DOI: 10.1016/j.apsoil.2006.03.010.
 Megharaj M, Kantachote D, Singleton I, Naidu R. Effect of long-term contamination of DDT on soil microflora with special reference to soil algae and algae transformation DDT. Environ Pollut. 2000;109:35-42. DOI: 10.1016/S0269-749(99)00231-6.
 Cai X, Sheng G, Liu W. Degradation and detoxication of acetochlor in soils treated by organic and thiosulfate amendments. Chemosphere. 2007;66:286-292. DOI: 10.1016/j.chemosphere.2006.05.011.
 Nweke CO, Ntinugwa C, Obah IF, Ike SC, Eme GE, Opara EC, et al. In vitro effects of metals and pesticides on dehydrogenase activity in microbial community of cowpea (Vigna unguiculata) rhizoplane. Afr J Biotechnol. 2007;6(3):290-295.
 Yang-Fang Y, Hang M, Xiang-Chi Z. Effects of mefenacet on microbial respiration and enzyme activities in paddy soil. Acta Pedol Sin. 2004;41:93-96.
 Sukul P. Enzymatic activities and microbial biomass in soil as influenced by metaxyl residues. Soil Biol Biochem. 2006;38:320-326. DOI: 10.1016/j.soilbio.2005.05.009.
 Yao X, Min H, Lii Z, Yuan H. Influence of acetamipirid on soil enzymatic activities and respiration. Eur J Soil Biol. 2006;42:120-126. DOI: 10.1016/j.ejsobi.2005.12.001.
 Wyszkowska J. Effect of soil contamination with Treflan 480 EC on biochemical properties of soil. Pol J Environ Stud. 2002;11(1):71-77.
 Wyszkowska J, Kucharski J. Biochemical and physicochemical properties of soil contaminated with herbicide Triflurotox 250 EC. Pol J Environ Stud. 2004;13(2):223-231.
 Bécaert V, Samson R, Deschênes L. Effect of 2,4-D contamination on soil functional stability evaluated using the relative soil stability index (RSSI). Chemosphere. 2006;64:1713-1721. DOI: 10.1016/j.chemosphere.2006.01.008.