Anti-inflammatory and analgesic activities of methanol extract of Helianthus annuus Linn. (Asteraceae) leaf

Open access


This study evaluate the anti-inflammatory and analgesic properties of Helianthus annuus Linn. in rats. Methanol extract of Helianthus annuus (HAE) leaf was used in this study. Formalin- and egg-albumin induced-paw edema were used to investigate the anti-inflammatory activities while acetic acid-induced writhing reflex and tail flick models were used to evaluate the analgesic properties. The doses of HAE used were 150, 300 and 600 mg/kg. Acetylsalicylic acid (ASA) was used as reference drug in the anti-inflammatory and writhing reflex models while pentazocine (reference drug) was used in tail flick model. The negative control was dosed 5% tween-20 (10 ml/kg). The HAE exhibited significant (P < 0.05) dose-dependent anti-inflammatory and analgesic activities. At 3 hour (h) post treatment, the HAE (300 mg/kg) produced 33.33% and 32.94% while ASA produced 36.36% and 35.29% reduction in paw volume in the formalin and egg-albumin induced paw edema models respectively when compared with negative control. In the acetic acid-induced writhing reflex, ASA and HAE (600 mg/kg) produced 67.89% and 35.78% reduction in the number of writhing, respectively when compared with the negative control. Pentazocine and HAE (300 mg/kg) caused 67.62% and 35.24% increase in pain reaction time when compared with the negative control. The study affirms the folkloric uses of Helianthus annuusin the management of pain and inflammation.

1. McGonagle D, McDermott MF. A proposed classification of the immunological diseases. PLoS medicine 2006; 3(8): e297.

2. Raicevic G, Najar M, Najimi M, El Taghdouini A, Van Grunsven LA, Sokal E, et al. Influence of inflammation on the immunological profile of adult-derived human liver mesenchymal stromal cells and stellate cells. Cytotherapy 2015; 17(2): 174-185.

3. Heymann F, Peusquens J, Ludwig-Portugall I, Kohlhepp M, Ergen C, Niemietz P, et al. Liver inflammation abrogates immunological tolerance induced by Kupffer cells. Hepatology 2015; 62(1): 279-291.

4. Ajayi AM, Ologe MO, Ben-Azu B, Okhale SE, Adzu B, Ademowo OG. Ocimum gratissimum Linn. Leaf extract inhibits free radical generation and suppressed inflammation in carrageenan-induced inflammation models in rats. J Basic Clin Physiol Pharmacol 2017; 28(6): 531-41.

5. Lee HJ, Shin JS, Lee KG, Park SC, Jang YP, Nam JH, et al. Ethanol Extract of Potentilla supina Linne Suppresses LPS-induced Inflammatory Responses through NF-κB and AP-1 Inactivation in Macrophages and in Endotoxic mice. Phytother Res 2017; 31(3); 475-487.

6. Shahpiri Z, Bahramsoltani R, Farzaei MH, Farzaei F, Rahimi R. Phytochemicals as future drugs for Parkinson’s disease: a comprehensive review. Rev Neurosciences 2016; 27(6): 651-68.

7. Onoja SO, Udem SC, Anaga AO. Effect of chronic administration of hydromethanol leaf extract of Helianthus annuus on Erythrocytic Profile in Normal Rats. J Nutr Ther 2017; 6(2): 59-63.

8. Bashir T, Zahara K, Haider S, Tabassum S. Chemistry, pharmacology and ethnomedicinal uses of Helianthus annuus (Sunflower): A review. Pure and Applied Biology 2015; 4(2): 226-235.

9. Eze VC, Onoja SO, Ezeja MI, Eze UA. In vitro antibacterial, antioxidant and phytochemical analysis of Helianthus annuus leaves extract on some bacteria causing infection. Int J Pharm Pharm Res 2015; 4(1): 93-100

10. Onoja SO, Anaga AO. Bioassay-guided fractionation, antihyperglycemic and antioxidant properties of the methanol leaf extract of Helianthus annuus. International Journal of Pharmacognosy and Phytochemical Research 2015; 7(2): 340-346.

11. Dwivedi A, Sharma GN, Kaushik AY. Evaluation of Helianthus annuus L. leaves extract for antidiarrheal and antihistaminic activity. Int J Res Ayurveda Pharm 2015; 6(1): 118-124.

12. Ogunwande IA, Flamini G, Cioni PL, Omikorede O, Azeez RA, Ayodele AA, et al. Aromatic Plants growing in Nigeria: Essential Oil Constituents of Cassia alata (Linn.) Roxb. and Helianthus annuus L. Rec Nat Prod 2010; 4(4): 211-217

13. Macías FA, Lopez A, Varela RM, Molinillo JM, Alves PL, Torres A. Helivypolide G. A novel dimeric bioactive sesquiterpene lactone. Tetrahedron lett 2004; 45(35): 6567-6570.

14. Ezeja MI, Omeh YN, Onoja SO, Ukaonu IH. Anti-inflammatory and antioxidant activities of the methanolic leaf extract of Cissus aralioides. Am J Pharmacol Sci 2015; 3(1): 1-6.

15. Onoja SO, Ezeja MI, Omeh YN, Onwukwe BC, Antioxidant, anti-inflammatory and antinociceptive activities of methanolic extract of Justicia secunda Vahl leaf, Alexandria J. Med. 2017; 53: 207-213.

16. Evans WC. Trease and Evans Pharmacognosy, 16th edition, Saunders Elsevier, Edinburgh, 2009; 87-89.

17. Onoja SO, Anaga AO. Evaluation of the antidiabetic and antioxidant potentials of methanolic leaf extract of Helianthus annuus L. on alloxan-induced hyperglycemic rats. Comp Clin Patho 2014; 23: 1565-73.

18. Carrillo W, Spindola H, Ramos M, Recio I, Carvalho JE. Anti-Inflammatory and Anti-Nociceptive Activities of Native and Modified Hen Egg White Lysozyme. J Med Food 2016; 19(10): 978-82.

19. Krishnaraju AV, Rao CB, Sundararaju D, Sengupta K, Trimurtulu G. Anti-inflammatory activity of Vitex leucoxylon L. bark extracts against Freund’s complete adjuvant induced arthritis in Sprague Dawley rat. Am J Infect Dis 2009; 5: 68-73.

20. Sofowora EA. Medicinal plants and traditional medicine in Africa. 3rd ed. Nigeria: Spectrum books Ltd. 2008.

21. Vogel HG. Editor. Drug discovery and evaluation: Pharmacological assays. 2nd ed. Berlin: Springer; 2002

22. Rang HP, Dale MM, Ritter JM, Moore RK. Pharmacology. 6th Edition. Edinburgh: Elsevier Churchill Livingstone. 2009.

23. Rashidian A, Mehrzadi S, Ghannadi AR, Mahzooni P, Sadr S, Minaiyan M. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: a light microscopic evaluation. J Integr Med 2014; 12(2): 115-20.

24. Shelmadine BD, Bowden RG, Moreillon JJ, Cooke MB, Yang P, Deike E, et al. A Pilot Study to Examine the Effects of an Anti-inflammatory Supplement on Eicosanoid Derivatives in Patients with Chronic Kidney Disease. J Altern Complement Med 2017; 23(8): 632-8.

25. Ahmad SF, Attia SM, Bakheet SA, Zoheir KM, Ansari MA, Korashy HM, et al. Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines. Inflammation 2015; 38(2): 846-57.

26. Yang EJ, Hyun KH, Kim H, Kim MJ, Lee NH, Hyun CG. Acanthopanax koreanum roots inhibit the expression of pro-inflammatory cytokines, inducible nitric oxide synthase, and cyclooxygenase-2 in RAW 264.7 macrophages. Orient J Chem 2016; 32(1): 29-35.

27. Murakami M, Kudo I. Recent advances in molecular biology and physiology of the prostaglandin E2-biosynthetic pathway. Prog Lipid Res 2004; 43: 3–35.

28. Wheeler-Jones CPD, Farrar C, Garonna E. Protease-activated receptors, cyclo-oxygenases and pro-angiogenic signalling in endothelial cells. Biochem Soc Trans 2009; 37: 1179–83

Journal Information


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 42 42 42
PDF Downloads 50 50 50