Genetic bases of tomatо marker-assisted selection in Belarus

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Abstract

To develop tomato hybrids and varieties with a high fruit quality, we gradually solved the following tasks: development of DNA-marking methods for long shelf life genes; the genes modifying the biosynthesis of carotenoids and their composition; testing of the elaborated methods on the developed breeding material; the selection of samples with different allelic composition of fruit quality genes; the development of F1 hybrids using the method of successive crosses and their study; the selection of tomato forms by DNA-typing methods with target genes in F2 populations to develop valuable breeding samples; the study of carotenoids’ accumulation peculiarities and their inheritance.

We used DNA-identification methods for fruit quality genes: nor, rin, norA (long shelf life), B, ogc, hp2dg, gf-3 (carotenoid content). The tomato hybrids, combining two pigment content genes and one long shelf life gene and the model forms with different combinations of fruit quality genes (B/rin/gf-3, B/rin/hp2dg; B/nor/gf-3, B /nor/hp2dg; оgc/rin/gf-3, оgc/rin/hp2dg; оgc/nor/gf-3, оgc/nor/hp2dg) in a homozygous state were developed.

Use of the developed accessions with carotenoid content genes (ogc/hp2dg, B/hp2dg) as maternal forms and the accessions with complex fruit quality genes (ogc/hp2dg/nor, ogc/hp2dg/rin, B/hp2dg/nor, B/hp2dg/rin, ogc/gf-3/nor, ogc/gf-3/rin, B/gf-3/rin, B/gf-3/nor) as paternal forms for hybridization contributes to high accumulation of carotenoids and a lond period of fruit storability.

References

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