The analysis of microscopic fungi collection created at theDurmishidze Institute of Biochemistry and Biotechnology revealed 107 strains assimilating 2,4,6-TNT (2,4,6-trinitrotoluene) belonging to the different fungal genera. The strains have been isolated from the polluted areas adjacent to the military grounds and industrial waste waters. It has been shown TNT is degraded most actively by strains belonging to the following genera: Trichoderma, Aspergillus, Mucor and Trichoderma. Optimal cultivation conditions for highly active strains -the destructors of TNT have been revealed. It has been established that the carbon skeleton of TNT being utilized by the mentioned strains undergoes biotransformation. The existence of radioactive intermediates of biotransformation, organic acids (70-90%) and amino acids (10-30%) have been detected in liquid culture. Radioactive label of 1-14C-TNT is mostly found in fumaric acid, which is known as one of the main products of benzene biotransformation and further conversion into succinic acid. Remediation level of TNT-contaminated red and black soils treated by the most active strains Aspergillus nigerN2-2 and Mucor sp. T1-1 have been studied under laboratory and field conditions. Cultivation of the above mentioned strains under laboratory conditions in sterile, black and red soils for 30 days at 30°C allowed decreasing the content of TNT in black soil to the residual, and in red soil - to 15%; cultivation of Aspergillus niger N2-2 decreased the amount of TNT in black soil to 11 and in red soil - to 21%. Under field conditions, TNT degradation level in contaminated soils by naturally existing micro flora during 100 days was equal to 40-50%, and in the case of additional introduction of both fungal strains, TNT-destructors reached 80%.
1. Yateem A, Balba MT, Al-Awadhi H, El-Nawawy AS. White-rot fungi and their role in remediating oil-contaminated soil. Environment International1998; 24:181-187.
2. EPA. Health Effects Summary Tables. Annual FY-91. Prepared by thy office of health and Environmental Assessment, Environmental Criteria Remedial Repose. Washington, D. C. OERR 9200. 1991; a.6-303 (91-1). NTIS PB91-921199.
3. Freeman RA, Hileman FD, Noble RW, Schroy JM. In: J.H. Experiments on the mobility of 22.214.171.124-tetrachlorodibenzo-p-dioxin at Times Beach, Missouri ed. // Solving Hazardous Waste Problems, ACS Symposium 1987; Series Num. 338.
4. Yanders AF, Orazio CE, Puri RK, Kapila S. On translocation of 126.96.36.199 -tetrachlorodibenzo-p-dioxin: time dependent analysis at the Times Beach experimental site. Chemosphere 1989; 19(1-6):429-432.
5. Lee S, Lee SY, Shin KS. Biodegradation of 2,4,6-trinitrotoluene by White-Rot Fungus.Mycobiology 2009; 37(1): 17-20.
6. Solyanikova IP, Baskunov BP, Baboshin MA, Saralov AI, Golovleva LA. Detoxification of high concentrations of trinitrotoluene by bacteria. Appl. Biochem. Microbiol.2012; 48:21-27
7. Bayman P, Radkar GV. Transformation and tolerance of TNT (2, 4, 6-trinitrotoluene) by fungi. Int. Biodeter. Biodegr.1997; 39: 45-53.
8. Fomin GS, Fomin AG. Soil. Monitoring on quality and ecologic safety in accordance with international standards. Moscow, VNII standard), 2001; 86.
9. Waksman SA. Soil fungi and their activities. Soil., Sci 1916; 2(1) : 103-105.