Biotechnology, including genetic modifications, can play a vital role in helping to meet future food and environmental security needs for our growing population. The nature and use of biotechnology crops are described and related to aspects of food security. Biotechnological applications for food and animal feed are described, together with trends on global adoption of these crops. The benefits of biotechnology crops through increased yield, reduced pesticide use and decreased environmental damage are discussed. Examples of biotechnology crops which do not involve genetic modification are also described. Applications of biotechnology to drought and salt tolerance, and biofortification in which micronutrient content is enhanced are discussed. Emergent technologies such as RNA spraying technology, use of genome editing in agriculture and future targets for improved food and environmental security are considered.
1. Food and Agriculture Organisation of the United Nations. FAO Success Stories on Climate Smart Agriculture. FAO I3871E/1/05.14.
2. International Society for the Acquisition of Agricultural Applications. GM Crops and the Environment. Pocket K 4 2017.
3. Federoff NV. Food in a future of 10 billion. Agriculture and Food Security. 2015; 4: 11.
4. Food and Agriculture Organisation, United Nations Development Programme, World Programme for Food. The State of Food Insecurity in the World. http://www.fao.org/3/a-i4646e.pdf.2015.
5. International Society for the Acquisition of Agricultural Applications. Can Mother earth feed 9 + Billion by 2050? ISAAA Infographic 1. 2016. www.isaaa.org
6. International Society for the Acquisition of Agricultural Applications. Contribution of Biotech Crops to Sustainability. ISAAA Infographic 2. 2017. www.isaaa.org
7. Klumper W, Qaim M. A Meta-analysis of the impacts of genetically modified crops. PLoS ONE 2014; 9(11): e111629.
8. Brookes G, Barfoot P. GM Crops: global socio-economic and environmental impacts 1996-2015. 2017. PG Economics Ltd., UK, pp. 1-201.
9. James C. 20th Anniversary (1996-2015) of the Global Commercialisation of Biotech Crops and Biotech Crop Highlights in 2015. ISAAA Brief 51 2015. www.isaaa.org
10. James C. ISAAA Brief 52. 2016. www.isaaa.org
11. Stua M, Dearnley E What will BREXIT mean for the climate? The Conversation 2017; https://theconversation.com/what-will-brexit- mean-for-the-climate-clue-it-doesnt-look-good-87476
12. Gartland KMA. Responding to climate change: barriers to progress and green opportunities. Biochemist 2006; October 54-55.
13. Ruane J, Sonnino A. Agricultural biotechnologies in developing countries and their possible contribution to food security. J. Biotechnol. 2011; 156: 356-363.
14. Gartland KMA, Gartland JS. Green biotechnology for food security in climate change. Reference Module in Food Sciences 2016; Elsevier pp.1-9. http://dx.doi.org/10.1016/B978-0-08-100596-5.03071-7
15. US National Academies of Sciences, Engineering & Medicine. Genetically engineered crops: experiences and prospects. 2016. https://doi.org/10.17226/23395
16. Royal Society. GM Plants: questions and answers. 2016; DES3710. https://royalsociety.org/~/media/policy/projects/gm-plants/gmplant-q-and-a.pdf
17. American Council for Science and Health. Meta-analysis shows GM crops reduce pesticide use by 37 percent.
18. Guo D, Chen F, Inoue K et al. Downregulation of caffeic acid 3-O-methyltransferase and caffeoyl coA 3-O-methyltransferase in transgenic alfalfa: impacts on lignin structure and implications for the biosynthesis of G and S lignin. Plant Cell 2001; 13: 73-88.
19. Wechsler SJ, Milkove D. Genetically Modified Alfalfa Production in the United States. 2017; United States Department of Agriculture Economic Research Service. https://www.ers.usda.gov/amber-waves/2017/may/genetically-modified-alfalfa-production-in-the-united-states/
20. Brookes G, Taheripour F, Tyner WE. The contribution of glyphosate to agriculture and potential impact of restrictions on use at the global level. GM Crops and Food 2017; https://doi.org/10.1080/21645698.2017.1390637
21. United States Dept. of Agriculture Biotechnology Consultation - Note to File BNF 000153 2017. https://www.fda.gov/Food/IngredientsPackagingLabeling/GEPlants/Submissions/ucm542339
22. Rommens CM, Yan H, Swords K et al. Low-acrylamide French fries and potato chips. Plant Biotechnology Journal 2008; 6:843-853.
23. Simplot Plant Sciences 2017. Innate second generation potatoes with late blight protection receive EPA and FDA clearances. http://www.simplot.com/plant_sciences
24. Halterman D, Guenthner J, Collinge S et al. Biotech crops in the 21st century: 20 years since the first biotech potato. Am. J. Potato Res. 2016; 93: 1-20.
25. Armen, J. Arctic apples: Leading the ‘next wave’ of biotech foods with consumer benefits. Australasian Biotechnology, 2015; 25: 50. No. 2, http://search.informit.com.au/documentSummary;dn=296007511823496;res=IELHEAISSN:1036-7128.
26. Smyth SJ. Canadian regulatory perspectives on genome engineered crops. GM Crops and Food 2017; 8: 35-43.
27. Silva KJP, Brunings AM, Pereira JA et al. The Arabidopsis ELP/ELO3 and ELP4/ELO1 genes enhance disease resistance in Fragaria vesca. BMC Plant Biology 2017; 17:230.
28. Van Der Straeten D, Fitzpatrick TB, De Steur H Biofortification of crops: achievements future challenges, socio-economic, health and ethical aspects. Curr. Op. Biotech. 2017; 44:vii-x.
29. Barreca N. Biofortification pioneers win 2016 World Food Prize for fight against malnutrition. 2016; World Food Prize Organisation 2016; https://www.worldfoodprize.org/index.cfm/87428/40322/biofortification_pioneers_win_2016world_food_prize
30. Blancquaert D, Van Daele J, Strobbe S et al. Improving folate (vitamin B9) stability in biofortified rice through metabolic engineering. Nature Biotechnology 2015; 33: 1076-1078.
31. Li K-T, Moulin M, Mangel N et al. Increased bioavailable vitamin B6 in field grown transgenic cassava for dietary sufficiency. Nature Biotechnology 2015; 33: 1029-1032.
32. Giuliano G. Provitamin A biofortification of crop plants: a gold rush with many miners. Current Opinion in Biotechnology 2017; 44: 169-182.
33. Potrykus I. “Golden Rice”, a GMO-product for public good, and the consequences of GE-regulation. J of Plant biochemistry and biotechnology 2012; 21S: 68-75.
34. Golden Rice Project 2017. http://www.goldenrice.org
35. Stone GD, Glover D. Disembedding grain: Golden rice, the Green Revolution and heirloom seeds in the Philippines. Agriculture and Human Values 2017; 34: 87-102.
36. Tang G, Qin J, Dolnikowski GG et al. Golden Rice is an effective source of vitamin A. American Journal of Clinical Nutrition 2009; 89: 1776-1783.
37. De Steur H, Mehta S, Gellynck X et al. GM biofortified crops: potential effects on targeting the micronutrient intake gap in human populations. Current opinion in Biotechnology 2017; 44: 181-188.
38. Paine JA, Shipton CA, Chaggar S, et al. Improving the nutritional value of Golden Rice through increased pro-vitamin A content. Nature Biotechnology 2005; 23:482-487.
39. Brooks S. Biofortification: Lessons from the Golden Rice Project. Food Chain 2013; 3: 77-88.
40. Kava R. All I want for Christmas is Golden Rice. American Council for Science and Health News 2017; 08.12.2017. https://www.acsh.org/news/2017/12/08/all-i-want-christmas-golden-rice-12251
41. World Health Organisation. Micronutrient deficiencies: Vitamin A deficiency 2017; http://www.who.int/nutrition/topics/vad/en/
42. UNICEF Data. East Asia and the Pacific achieved the highest twodose coverage with vitamin A supplements of all regions in 2015. December 2017; https://data.unicef.org/topic/nutrition/vitamin-a-deficiency/
43. Kava R. Move over, Golden rice- Golden potatoes are on the way. American Council for Science and Health News 2017; 13.11.2017. https://www/acsh.org/news/2017/11/13/move-over-goldenrice-%2%80%94-golden-potatoes-are-way-12136
44. Chitchumroonchokchai C, Diretto G, Parisi B et al. Potential of golden potatoes to improve vitamin a and vitamin E status in developing countries. PLoSONE 2017; 12 (11): e0187102. https://doi.org/10.1371/journal.pone.0187102
45. Che P, Zhao Z-Y, Glassman K et al. Elevated vitamin E content improves all-trans β-carotene accumulation and stability in biofortified sorghum. PNAS (USA) 2016; 113: 11040-11045
46. Report G. Investing in the future- A united call to action on vitamin and mineral deficiencies. 2009; http://www.unitedcalltoaction.org/index.asp
47. Blancquaert D, De Steur H, Gellynck X et al. Metabolic engineering of micronutrients in crop plants. Annals New York academy Sciences (2017) 1390: 59-73.
48. Waltz E. Vitamin A Super Banana in human trials. Nature Biotechnology 2014; 32: 857.
49. Paul J-Y, Khanna H, Kleidon J et al. Golden bananas in the field: elevated pro-vitamin A from the expression of a single banan transgene. Plant Biotech. J. 2017; 15: 520-532.
50. Mbabazi R. Molecular characterisation and carotenoid quantification of pro-vitamin A biofortified genetically modified bananas in Uganda. PhD Thesis. 2015; Queensland University of Technology.
51. Buah S, Mlalazi B., Khanna H, Dale JL and Mortimer CL. The quest for golden bananas: investigating carotenoid regulation in a Fe’i group Musa cultivar. J. Agric. Food Chem. 2016; 64: 3176-3185.
52. Dhandapani R, Singh VP, Arora A et al. Differential accumulation of β-carotene and tissue specific expression of phytoene synthase (MaPSy) gene in banana (Musa sp.) cultivars. J Food Sci. technol. 2017; 54: 4416-4426.
53. Water Efficient Maize for Africa. 2017; https://wema.aatf-africa.org/about-wema-project
54. Xu J, Yuan Y, Xu Y et al. Identification of candidate genes for drought tolerance by whole-genome resequencing in maize. BMC Plant Biology 2014; 14: 83.
55. African Agricultural Technology Foundation. DroughtTEGO WE1101 Drought-tolerant maize hybrid. 2017; http://www.aatf-africa.org
56. Morsy M. Microbial symbionts: a potential bio-boom. J. Investig. Genomics 2015; 2: 00015.
57. Castiglioni P, Warner D, Bensen RJ et al. Bacterial RNA chaperones confer abiotic stress tolerance. Plant Physiology. 2008; 147: 446-455.
58. Nuccio ML, Wu J, Mowers R et al. Expression of tehalose-6-phosphate phosphatase in maize ears improves yield in well-watered and drought conditions. Nature Biotechnology. 2015; 33: 862-869.
59. Adee E. Drought-tolerant corn hybrids yield more in droughtstressed environments with no penalty in non-stressed environments. Frontiers in Plant Science. 2016; 13 Oct 2016.
60. Rea-hybrids. Introducing Genuity DroughtGard hybrids. 2017; http://www.rea-hybrids.com
61. Siegfried BD, Hellmich RL. Understanding successful resistance management: the European corn borer and Bt corn in the United States. GM Crops Food. 2012; 3:184-193.
62. Ammann K The impact of agricultural biotechnology on biodiversity. (2004) Botanic gardens, University of Bern.
63. Salt tolerance of plants. University of Alberta Agriculture and Forestry (2017). http://www1.agric.gov.ab.ca/$department/deptdocs.nsf/all/agdex3303
64. Tilbrook J, Schilling RK, Berger B et al. Variation in shoot tolerance mechanisms not related to ion toxicity in barley. Functional Plant Biology (2017) 14: 1194-1206.
65. Zou C, Chen A, Xiao L et al. A high-quality genome assembly of quinoa provides insightsinto the molecular basis of salt bladder- based salinity tolerance and exceptional nutritional value. Cell Research (2017) DOI: 10.1038/cr.2017.124.
66. Rakshit S. The Handbook of Plant Mutation Screening: Mining of natural and induced alleles. Wiley-VCH (2010) pp. 185-197.
67. Takagi H, Tamiru M, Abe A et al. MutMap accelerates breeding of a salt-tolerant rice cultivar. Nature Biotechnology (2015) 33: 445-449.
68. Trapnell C, Pachter L, Salzberg SL. TopHat: discovering splice junctions with RNA-seq. Bioinformatics (2009)25: 1105-1109.
69. Goswani K, Tripathi A, Sanan-Mishra N. Comparative miRomics of salt-tolerant and salt-sensitive rice. J Integrative bioinformatics (2017) 2017002.
70. Tan GC, Chan E, Molnar A et al. 5’-isomiR variation is of functional and evolutionary importance. Nucleic Acids Research (2104) 42: 9424-9435.
71. Morin RD, O’Connor MD, Griffith M et al. Application of massively parallel sequencing to microRNA profiling and discovery in human embryonic stem cells”. Genome Research (2008); 18: 610-621.
72. Regalado A. The next great GMO debate. MIT Technology Review (2015) https: //www.technologyreview.com/s/540136/the-nextgreat- gmo-debate
73. Shew AM, Danforth DM, Nalley LL et al. New innovations in agricultural biotech: consumer acceptance of topical RNAi in rice production. Food Control (2017) 81: 189-195.
74. Shan Q, Wang Y, Li j et al. Genome editing in rice and wheat using the CRISPR/Cas9 system. Nature Protocols (2014) 9: 2395-2410.
75. Gartland KMA, Dundar M, Beccari T et al. Advances in biotechnology: genomics and genome editing. EuroBiotech Journal (2017) 1:1-8.
76. Ricroch A, Clairand P, Harwood W Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture. Emerging Topics in Life Sciences (2017) 1: 169-182.
77. LeBlanc C, Zhang F, Mendez J et al. Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress. Plant Journal (2017) DOI: 10.1111/tpj.13782
78. Shen H, Zhong X, Zhao F et al. Overexpression of receptor-like kinase ERECTA improves thermotolerance in rice and tomato. Nature Biotechnology (2015) 33: 996-1003.
79. Nuccio ML, Wu J, Mowers R et al. Expression of trehalose-6-phosphate phosphatase in maize ears improves yields in well-watered and drought conditions. Nature Biotechnology (2015) 33: 862-869.
80. Yang X, Hu R, Tuskan GA et al. The Kalanchoe genome provides insights into crassulacean acid metabolism. Nature Communications (2017) 8: 1899.