Moringa oleifera (drumstick tree) seed coagulant protein (MoCP) binds cadmium - preparation and characterization of nanoparticles

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Moringa oleifera is grown globally. It is a multipurpose tree and the seeds are rich in phytochemicals with antimicrobial activities. The crude powder of seeds clarify the turbid and metal contaminated water. M. oleifera (drumstick tree) seed coagulant protein (MoCP) was isolated to homogeneity from the crude extracts by carboxymethyl cellulose chromatography (CMC) and gel filtration. The molecular weight of the protein on gel filtration was 13 kDa and in SDS-PAGE it migrated as a single band under reducing conditions with molecular mass of 6.5 kDa (dimeric). Immobilized MoCP selectively binds cadmium from aqueous solutions (pH 2.0-7.0) with maximum binding at pH 6.0 in 180 min when tested at 10-600 minutes. It also bound the metal in the concentration range of 30-70mgL-1. The adsorption kinetics was better described by pseudo second order and the data better explained by freundlich isotherm model than Langmuir isotherm model as in Freundlich model the correlation coefficient (R2) is high and the calculated qmax is very close to the experimental qmax rather than Langmuir isotherm model. Furthermore, the nanoparticles of MoCP were prepared and characterized using transmission electron microscopy (TEM). The authenticity of the isolated protein and the nanopraticles prepared was confirmed by specific reactivity with the MoCP antibody raised earlier in our laboratory.

1. Lalhruaitluanga H and Prasad MNV (2015) Removal of heavy metals from aqueous solutions through biosorption. In, Current applications of biotechnology, In Eds. Dündar M, Bruschi F, Gartland K, Magni MV, Peter Gahan P and Deeni Y. Erciyes University, Kayseri, Turkey. 87-102

2. Adelaja OA, Amoo IA, Aderibigbe AD. Biosorption of Lead (II) ions from aqueous solution using Moringa oleifera pods. Archives of Applied Science Research 2011; 3(6), 50-60.

3. Agbahoungbata MY, Fatombi JK. Removal of reactive dyes from their aqueous solutions using Moringa oleifera seeds and Grewia venusta peel. Desalination and Water Treatment 2016; 57(47): 22609-22617.

4. Akhtar M, Hasany SM, Bhanger, MI, Iqbal, S, Sorption potential of Moringa oleifera pods for the removal of organic pollutants from aqueous solutions. Journal of Hazardous Material 2007; 141, 546-556.

5. Al-Anizi AA, Hellyer MT, Zhang D. Toxicity assessment and modelling of Moringa oleifera seeds in water purification by whole cell bioreporter Water Research 2014; 56, 77-87

6. Alves VN, Coelho NMM. Selective extraction and preconcentration of chromium using Moringa oleifera husks as biosorbent and flame atomic absorption spectrometry Microchemical Journal 2013; 109, 16-22

7. Amadi N, Tanee FBG. Efficacy of Moringa oleifera as a phytoextraction plant in the remediation of heavy metals polluted soil. Afr J Plant Sci 2014; 8(12): 546-553.

8. Cai Y, Luo Y, Dong H, Zhao X, Zheng M. Hierarchically porous carbon nanosheets derived from Moringa oleifera stems as electrode material for high-performance electric double-layer capacitors Journal of Power Sources 2017; 353 (15) 260-269

9. Sharma P. Removal of Cd (II) and Pb (II) from aqueous environment using Moringa oleifera seeds as biosorbent: a low cost and ecofriendly technique for water purification Trans. Indian Inst Met 2008; 61(2-3) 107-110

10. Daniel IB, Agho MO. Alarming fluoride concentrations of water samples from boreholes in Bama community and remediation with Moringa oleifera. Int J Water Soil Res 2004; 1(1): 13.

11. Freitas JH, de Santana KV, do Nascimento AC, de Paiva SC, de Moura MC, Coelho LC, de Oliveira MB, Paiva PM, do Nascimento AE, Napoleão TH. Evaluation of using aluminum sulfate and water soluble Moringa oleifera seed lectin to reduce turbidity and toxicity of polluted stream water. Chemosphere 2016; 163, 133-141.

12. George KS, K. Revathi KB, Deepa N, Sheregar CP, Ashwini TS, Das S. a study on the potential of Moringa leaf and bark extract in bioremediation of heavy metals from water collected from various lakes in Bangalore, Procedia Environmental Sciences, 2016; 35, 869-880

13. Gupta S, Jain R, Kachhwaha S, Kothari SL. Nutritional and medicinal applications of Moringa oleifera Lam.-Review of current status and future possibilities. Journal of Herbal Medicine, 2017In press, accepted manuscript, available online 2 August

14. Kardam A, Raj KR, Arora KJ, Srivastava MM, Srivastava S, Artificial neural network modeling for sorption of cadmium from aqueous system by shelled Moringa oleifera Seed Powder as an Agricultural Waste. Journal of Water Resource and Protection 2010; 2, 339-344.

15. Kerdsomboon K, Tatip S, Kosasih S, Auesukaree C. Soluble Moringa oleifera leaf extract reduces intracellular cadmium accumulation and oxidative stress in Saccharomyces cerevisiae Journal of Bioscience and Bioengineering 2016; 121, 543-549

16. Mataka LM, Henry EMT, Masamba WRL, Sajidu SM. Lead remediation of contaminated water using Moringa stenopetala and Moringa oleifera seed powder. International Journal of Environmental Science and Technology 2006; 3(2), 131-140.

17. Matouq M, Jildeh N, Qtaishat M, Hindiyeh M, Al Syouf MQ. The adsorption kinetics and modeling for heavy metals removal from wastewater by Moringa pods Journal of Environmental Chemical Engineering 2015; 3, 775-784

18. Santos TR, Silva MF, Nishi L, Vieira AM, Klein MR, Andrade MB, Vieira MF, Bergamasco R.. Development of a magnetic coagulant based on Moringa oleifera seed extract for water treatment. Environ Sci Pollut Res 2016; 23(8):7692-700

19. Ramabulana T, Mavunda RD, Steenkamp PA, Piater LA, Dubery IA, Madala NE.Perturbation of pharmacologically relevant polyphenolic compounds in Moringa oleifera against photooxidative damages imposed by gamma radiation. Journal of Photochemistry and Photobiology B Biology 2016; 156, 79-86

20. Reddy DHK, Harinatha Y, Seshaiaha K, Reddy AVR Biosorption of Pb(II) from aqueous solutions using chemically modified Moringa oleifera tree leaves. Chemical Engineering Journal 2010; 162, 626-634.

21. Reddy DHK, Seshaiaha K, Reddy AVR. Leec SM. Optimization of Cd(II), Cu(II) and Ni(II) biosorption by chemically modified Moringa oleifera leaves powder. Carbohydrate Polymers 2012; 88, 1077-1086.

22. Reddy DHK, Seshaiah K., Reddy AVR, Rao MM, Wang MC. Biosorption of Pb2+ from aqueous solutions by Moringa oleifera bark: equilibrium and kinetic studies. Journal of Hazardous Materials 2010; 174, 831-838.

23. Sahabi DM, Magaji UF, Abubakar MK, Muhammad AB. Biosorption Activity of Moringa oleifera Seeds Powder. International Journal of Innovative Research & Development. 2014; 3(13) 245-251

24. Sajidu SM, Henry EMT, Kwamdera G, Mataka L, Removal of lead, iron and cadmium ions by means of polyelectrolytes of Moringa oleifera whole seed kernel. WIT Transactions on Ecology and the Environment 2005; 80, 251-258.

25. Sajidu SMI, Henry EMT, Persson I, Masamba WRL, Kayambazinthu D. pH dependence of sorption of Cd 2+ , Zn 2+, Cu 2+ and Cr 3+ on crude water and sodium chloride extracts of Moringa stenopetala and Moringa oleifera. African Journal of Biotechnology 2006; 5(23), 2397-2401.

26. Santos AFS, Paiva PMG, Teixeira JA, Brito AG, Coelho LCBB., Nogueira RB Coagulant properties of Moringa oleifera protein preparations: application to humic acids removal. Environmental Technology 2012; 33(1-3), 69-75.

27. Sharma P, Kumari P, Srivastava MM, Srivastava S, Removal of cadmium from aqueous system by shelled Moringa oleifera Lam. seed powder. Bioresource Technology 2006; 97(2), 299-305.

28. Suthari S, Prasad MNV. Moringa oleifera: A potential agroforestry tree can meet the nutritional requirements of rural people. (Chapter 19); pp. 489-512. In: Dagar and Tewari (eds.), Agroforestry for Sustainable Development. USA: Nova Science Publishers, 2016. (ISBN: 978-1-63485-094-0).

29. Tie J, Jiang M, Li H, Zhang S, Zhang X. A comparison between Moringa oleifera seed presscake extract and polyaluminum chloride in the removal of direct black 19 from synthetic wastewater Industrial Crops and Products, 74, 530-534

30. Veronica O, Florence N, Habauka MK. A study of the removal of heavy metals from aqueous solutions by Moringa oleifera seeds and amine-based ligand 1,4-bis[N,N-bis(2-picoyl)amino]butane. Analytica Chimica Acta 2012; 730, 87-92.

31. Saif MMS, Kumar NS, Prasad MNV. Binding of cadmium to Strychnos potatorum seed proteins in aqueous solution: Adsorption kinetics and relevance to water purification, Colloids Surf B: Biointerfaces 2012; 94, 73-79.

32. Fu F, Wang Q. Removal of heavy metal ions from wastewaters: a review. J Environ Manage 2011; 92(3): 407-418.

33. Demirbas, A. Heavy metal adsorption onto agro-based waste materials: a review. J Hazard Mater 2008; 157(2-3): 220-229

34. Krishnani, KK, Ayyappan S. Heavy metals remediation of water using plants and lignocellulosic agrowastes. Rev Environ Contam Toxicol 2006; 188: 59-84.

35. Saif MMS, Kumar NS, Prasad MNV. Plant based excipients - The case of Strychnos potatorum seed polysaccharide nanoparticles. Pharma Bio World 2014; (11) 44-48.

36. Ngah, WSW, Hanafiah MA. Removal of heavy metal ions from wastewater by chemically modified plant wastes as adsorbents: a review. Bioresour Technol 2008; 99(10), 3935-3948.

37. Gassenschmidt U, Jany KD, Tauscher B, Niebergall H. Isolation and characterization of a flocculating protein from Moringa oleifera Lam. Biochim Biophys Acta 1995; 1243(3), 477-481.

38. Ghebremichael KA, Gunaratna KR, Henriksson H, Brumer H, Dalhammar G. A simple purification and activity assay of the coagulant protein from Moringa oleifera seed. Water Res 2005; 39(11): 2338-2344

39. Tejavath KK, Nadimpalli SK. Understanding the roles of proteins in plant seeds with gel electrophoresis. Separation Science 2010; 2(2): 20-26

40. Venugopal A, Kumar BSG, Mohiddon MA, Krishna MG, Kumar NS. Facile synthesis of biodegradable affinity-purified lectin nanoparticles. Cogent Chemistry 2016; 2(1): 1230951.

41. Saif MMS, Khan I, Prasad MNV, Kumar NS. Preparation and Characterization of Strychnos potatorum L. seed polysaccharide nanoparticles and affinity matrices: relevance to biological applications. Adv Sci Eng and Med 2014; 6, 1-8.

42. Sajithkumar K.. Visakh, et al J.PM.. Moringa oleifera (Drum Stick Vegetable Fibre) Based Nanocomposites with Natural Rubber: Preparation and Characterizations. Waste and Biomass Valorization 2016; 7(5): 1227-1234

43. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 1970; (5259): 680-685.

44. Krishna ADS, Mandraju RK, Kishore G, and Kondapi AK (2009)An efficient targeted drug delivery through apotransferrin loaded nanoparticles. PLoS One. 2009; 4(10): e7240.

45. Leone A, Spada A, Battezzati A, Schiraldi A. Cultivation, Genetic, Ethnopharma-cology, Phytochemistry and Pharmacology of Moringa oleifera Leaves: An Overview. Int J Mol Sci 2015, 16(6): 12791-12835.

46. Tejavath KK, Kumar NS. Purification and characterization of a class II alpha-Mannosidase from Moringa oleifera seed kernels. Glycoconj J 2014; 31(6-7): 485-496.

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