Antifungal activity of a biosurfactant-producing lactic acid bacteria strain

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Lactic acid bacteria are frequently utilized in food industry and they are also recognized as antimicrobial agents due to their capability to produce metabolites such as: organic acids, biosurfactants, bacteriocins, hydrogen peroxide, cyclic dipeptides, exopolysaccharides. The main goal of this paper was to present the results of the research carried out on the strain LCM2 of lactic acid bacteria isolated from brined cucumbers, for production of biosurfactants and to assess its antifungal properties. The emulsification capacity of biosurfactant was measured using kerosene as the hydrophobic substrate. The value of emulsification index E24 was 89.04% showing a high emulsification activity of the biosurfactant. The structural characterization of biosurfactant by TLC revealed its glycolipidic nature. Assay of the ionic charge established the anionic charge of the biosurfactant revealed by the presence of precipitation lines towards the cationic surfactant dodecyl-dimethyl-ammonium chloride. The biosurfactant presented antibiofilm activity with low adherence capacity, structural damages of the hyphal net, conidiophores and delays or lack of sporulation and decreased biomass accumulation in four mycotoxigenic Penicillium and Aspergillus isolates. Results of in vitro assays recommend the biosurfactant produced by the new lactic acid bacteria strain LCM2 for biotechnological purposes, as alternative antifungal agent in food industry.

1. Crowley S, Mahony J, & van Sinderen D. Comparative analysis of two antifungal Lactobacillus plantarum isolates and their application as bioprotectants in refrigerated foods. J Appl Microbiol 2012; 113: 1417-1427.

2. Schnürer J, Magnusson J. Antifungal lactic acid bacteria as biopreservatives. Trends Food Sci Technol 2005; 16: 70-8.

3. Dalié D.K.D., Deschamps A.M., Richard-Forget F. Lactic acid bacteria-Potential for control of mould growth and mycotoxins: A review. Food Control 2010; 21: 370-380.

4. Zamfir M, Cornea CP, De Vuyst L, Grosu-Tudor SS. Biodiversity and biotehnological potential of lactic acid bacteria. Agro Life Sci J 2014; 3(1): 169-176.

5. Sumiardi A, Mangunwardoyo W, Hudiyono S, Susilaningsih D. Biosurfactant characterization of bacterial consortium from soil contaminated hydrocarbon in Cepu area, Central Java, Indonesia. Int J Sci Res Pub 2012; 2(7): 1-7.

6. Sharma D, Saharan BS. Functional characterization of biomedical potential of biosurfactant produced by Lactobacilllus helveticus. Biotechnol Rep 2015; 11: 27-35.

7. Rodrigues L, Banat IM, Teixeira J, Oliveira R. Biosurfactants: potential applications in medicine. J Antimicrob Chemother 2006; 57(4): 609-618.

8. Cooper GD, Goldenberg BG. Surface-Active Agents from Two Bacillus Species. Appl Environ Microbiol 1987; 53(2): 224-229.

9. Meylheuc T, van Oss CJ, Bellon-Fontaine MN. Adsorption of biosurfactants on solid surface and consequences regarding the bioadhesion of Listeria monocytogenes LO28. J Appl Microbiol 2001; 91: 822-832.

10. Sharma D, Saharan BS, Chauhan N, Procha S, Lal S. Isolation and functional characterization of novel biosurfactant produced by Enterococcus faecium. Springerplus 2015; 4(1): 4.

11. Adebayo CO, Aderiye BI. Antifungal activity of bacteriocins of lactic acid bacteria from some Nigerian fermented foods. Res J Microbiol 2010; 5: 1070-1082.

12. Saravanakumari P, Mani K. Structural characterization of a novel xylolipid biosurfactant from Lactococcus lactis and analysis of antibacterial activity against multi-drug resistant pathogens. Bioresour Technol 2010; 101(22): 8851-8854.

13. Padmapriya B, Suganthi S. Antimicrobial and antiadhesive activity of purified biosurfactants produced by Candida albicans. Middle East J Sci Res 2013; 14 (10): 1359-1369.

14. Techaoei S, Leelapornpisid P, Santiarwarn D, Lumyong S. Preliminary screening of biosurfactant producing microorganisms isolated from hot spring and garages in Northern Thailand. KMITL Sci Technol J 2007; 7: 38-43.

15. Patowary K, Patowary R, Kalita MC, Deka S. Characterization of biosurfactant produced during degradation of hydrocarbons using crude oil as sole source of carbon. Front Microbiol 2017; 8: 279.

16. Laref N, Guessas B. Antifungal activity of newly isolates of lactic acid bacteria. Innov Rom Food Biotechnol 2013; 13: 80-88.

17. El-Deeb N, Sharaf M, El-Adawi H. Antibacterial and plasmid curing activity of lactic acid bacteria against multidrug resistant bacteria strains. Int J Pharm 2015; 11(2): 114-121.

18. Ström K. Fungal inhibitory lactic acid bacteria - characterization and application of Lactobacillus plantarum MiLAB 393. Ph.D. Thesis, Swedish University of Agricultural Sciences, Uppsala, Sweden 2005.

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