Antimicrobial efficacy of some plant extracts on bacterial ring rot pathogen, clavibacter michiganensis ssp. sepedonicus

Open access


Control of plant bacterial diseases remains difficult due to the limited availability of efficient plant protection products with reduced negative effects either in the environment or with human and animal health. In order to reduce the usage of chemical pesticides alternative strategies for controlling plant pathogens and improve plant disease resistance are promoted. The aim of the study was to investigate the antibacterial activity of some natural compounds (plant extracts of Tamarix ramosissima, Rosmarinus officinalis, Chelidonium majus, Silybum marianum, Satureja hortensis essential oil and propolis) against bacterial ring rot pathogen, Clavibacter michiganensis ssp. sepedonicus (Cms). An agar diffusion method was used for the screening of the inhibitory effect of natural compounds on bacterial strains’ growth. Minimum inhibition concentrations (MICs) were determined by a twofold serial dilution method. The anti-pathogenic activity was investigated by the study of anti-biofilm activity of natural substances. The analyzed natural substances showed a good microbicidal activity and anti-biofilm activity. The results obtained from this study may contribute to the development of new bio-control agents as alternative strategies for prevention and control of ring rot pathogen.

1. EC (2000) Council Directive 2000/29/EC of 8 May 2000 on protective measures against the introduction into the Community of organisms harmful to plants or plant products and against their spread within the Community. Official Journal of the European Communities. L169, 1-112.

2. EC (1993) Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot. Official Journal of the European Communities L259, 1-25.

3. Gudmestad NC, Mallik I, Pasche JS, Anderson NR, and Kinzer K (2009) A Real-Time PCR Assay for the Detection of Clavibacter michiganensis subsp. sepedonicus Based on the Cellulase A Gene Sequence. Plant Disease, 93 (6): 649-659.

4. Gheorghies C, Cristea S (2001) Fitopatologie, 1, Ed. Ceres, Bucharest.

5. Cristea S (2005) Fitopatologie, 2, Ed Cris Book Universal Bucharest.

6. Manzer FE, Gudmestad NC, Nelson GA (1987) Factors affecting infection, disease development and symptom expression of bacterial ring rot. American Potato Journal, 64, 641-676.

7. Manzer FE, McKenzie AR (1988) Cultivar response to bacterial ring rot infection in Maine. American Potato Journal, 65, 333-339.

8. Pradhanang PM, Momol MT, Olson SM, Jones JB (2003) Effects of plant essential oils on Ralstonia solanacearum population density and bacterial wilt incidence in tomato. Plant Disease, 87, 423-427.

9. Kotan R, Cakir A, Dadasoglu F, Aydin T, Cakmakci R, Ozer H, Kordali S, Mete E, Dikbas N (2010) Antibacterial activities of essential oils and extracts of Turkish Achillea, Satureja and Thymus species against plant pathogenic bacteria. J. Sci. Food Agric., 90, 145-160.

10. EPPO (2006) PM 7/59 (1) Clavibacter michiganensis subsp. sepedonicus . Bulletin OEPP/EPPO 36, 99-109.

11. Badea M, Crasanda A, Chifiriuc MC, Marutescu L, Lazar V, Marinescu D, Olar R (2013) Synthesis, spectral and thermal study on new Fe(III) complexes with N,N-dimethylbiguanide as antibacterial agents. J. Therm. Anal. Calorim., 111 (5): 1743-1751.

12. Clinical and Laboratory Standards Institute (2012) Performance Standards for Antimicrobial Susceptibility Testing; Twenty Second Informational Supplement, M100-S22, 32 (3) Replaces M100-S21, 31 (1), Informational Supplement. CLSI: Wayne, PA, USA.

13. Limban C, Marutescu L, Chifiriuc MC (2011) Synthesis, spectroscopic properties and antipathogenic activity of new thiourea derivatives. Molecules,16 (9): 7593-7607.

14. Clinical and Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard-Ninth edition M07-A9, 32 (2), Replaces M07-A8, 29 (2). CLSI: Wayne, PA, USA.

15. Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, Beachey EH (1985) Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J. Clin. Microbiol., 22, 996-1006.

16. Christensen GD, Simpson WA, Younger JA et al. (1995) Adherence of coagulase negative Staphylococci to plastic tissue cultures: a quantitative model for the adherence of Staphylococci to medical devices. J Clin Microbiol., 22, 996-1006.

17. Drabu S, Chaturvedi S, Sharma M (2012) Tamarix gallica - an overview. Asian Journal of Pharmaceutical and Clinical Research, 5 (3).

18. Sultanova N, Makhmoor T, Abilov ZA, Parween Z, Omurkamzinova VB, ur-Rahman A, Choudhary MI (2001) Antioxidant and antimicrobial activities of Tamarix ramosissima. J Ethnopharmacol., 78(2- 3):201-5.

19. Cowan MM (1999) Plant Products as Antimicrobial Agents. Clinical Microbiology Reviews, 12 (4): 564-582.

20. Kotan R, Dadasoğlu F, Karagoz K, Cakir A, Ozer H, Kordali S, Cakmakci R, Dikbas N (2013) Antibacterial activity of the essential oil and extracts of Satureja hortensis against plant pathogenic bacteria and their potential use as seed disinfectants. Scientia Horticulturae, 153, 34-41.

Journal Information


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 192 192 20
PDF Downloads 78 78 14