Nanoparticle Tracking Analysis of Latex Standardized Beads

Open access

The most popular technique for particle size characterization is the dynamic light scattering (DLS). In recent years new advanced method based on counting each single particle movement was introduced giving perspective for measurement of each component of mixture. This study presents some advantages of nanoparticle tracking analysis (NTA) method in comparison to DLS. For tests standard polystyrene beds were chosen vary diameter of 22, 61 and 150 nm and its mixtures. Experiments showed that the particles size resolution allows to distinguish each population in two population suspension opposed to DLS. The NTA method permits to eliminate the negative effects i.e. dust or aggregates in sample during post processing, that permits to use it in a variety of studies.

Bangs Laboratories, Inc. (9.02.2014) http://www.bangslabs.com.

Berne B.J., Pecora R., (1976) Dynamic Light Scattering: with Applications to Chemistry, Biology, and physics, Wiley, New York.

Boyd RD, Pichaimuthu SK., Cuenat A. (2011) New approach to inter-technique comparisons for nanoparticle size measurements; using atomic force microscopy, nanoparticle tracking analysis and dynamic light scattering. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 387, 35-42.

Chatterjee J., Haik Y., Chen C. J. (2001) Modification and characterization of polystyrene-based magnetic microspheres and comparison with albumin-based magnetic microspheres, Journal of Magnetism and Magnetic Materials, 225, 21-29.

Faraji A., Wipf P. (2009) Nanoparticles in cellular drug delivery. Bioorganic & Medicinal Chemistry, 17, 29502962.

Filipe V., HaweA., Jiskoot W., (2010) Critical Evaluation of Nanoparticle Tracking Analysis (NTA) by NanoSight for the Measurement of Nanoparticles and Protein Aggregates, Pharmaceutical Research, 27, 796-810.

Gañán-Calvo A. M., Martín-Banderas L, González-Prieto R., Rodríguez-Gil A., Berdùn-Àlvarez T., Cebolla A., Chávez S., Flores-Mosquera M., (2006) Straightforward production of encoded microbeads by Flow Focusing: Potential applications for biomolecule detection, International Journal of Pharmaceutics, 324, 19-26.

Gennet N., Alexander L. M., Sánchez-Martín R. M., Behrendt J. M., Sutherland A. J., Brickman J. M., Bradley M. & Li M (2009) Microspheres as a vehicle for biomolecule delivery to neural stem cells, New Biotechnology, 25 (6), 442-449.

Jakubowicz J. (2008) Particle analysis and properties of mechanically alloyed Nd16Fe76-xTixB8, Superlattices and Microstructures, 43, 315-323.

Joubert MK, Luo Q, Nashed-Samuel Y, Wypych J, Narhi LO. (2011) Classification and characterization of therapeutic antibody aggregates, J Biol Chem., 286, 25118-2513.

Kanwal S., Traore Z. & Su X. (2010) Polystyrene microspheres based sandwich immunosensor using CdTe nanoparticles amplification and ultrasensitive flow-injection chemiluminescence detection, Colloids and Surfaces B: Biointerfaces, 81, 549-554.

Ma Q., Wang X., Li Y., Shi Y. & Su X. (2007) Multicolor quantum dot-encoded microspheres for the detection of biomolecules, Talanta, 72, 1446-1452.

NanoSight (2012) Technical data: NTA 2.3 Analytical Software, Operating Manual P554J, NanoSight Ltd., Minton Park, Amesbury, Wiltshire SP4 7RT, UK.

O'Hagan D. T. (1998) Microparticles and polymers for the mucosal delivery of vaccines, Advanced Drug Delivery Reviews, 34, 305-320.

Panyama J. & Labhasetwara V., (2003) Biodegradable nanoparticles for drug and gene delivery to cells and tissue Advanced Drug Delivery Reviews, 55, 329-347.

Ravichandran R. (2009) Nanotechnology-Based Drug Delivery Systems, Nanobiotechnol., 5, 17-33.