In this paper some selected applications of laser interferometry method in studies of biophysical model systems are presented. With the aid of a laser interferometric method, specific experiments were performed, which confirm that the gravitational field significantly modifies the amount of transported substance and affects the concentration profiles as well as the time evolution of the concentration field. The laser interferometry was also used to investigation of diffusion of antibiotics (ciprofloxacin or ampicillin) into the water phase from mixtures of neutral or negatively charged liposomes, and antibiotic-liposome interactions. Differences in the diffusion kinetics of ciprofloxacin and ampicillin from liposomal solutions to the water phase were observed. Moreover, the amount of ampicillin and ciprofloxacin released from the anionic liposomal phase was higher than that from the neutral one.
Dworecki K., Ślęzak A., Ornal-Wąsik B., Wąsik S. (2005). Evolution of concentration field in a membrane system, J. Biochem. Biophys. Methods, 62(2), 153 - 162.
Dworecki K., Ślęzak A., Drabik M., Ornal-Wąsik B., Wąsik S. (2006). Determination of the membrane permeability coefficient under concentration polarization conditions, Desalination, 198, 326-334.
Schatz A., Reitstetter R., Briegleb W., Linke-Hommes A. (1992). Gravity effects on biological systems, Adv. Space. Res. 12(1), 51-3.
Tosteson D.C.(1978). Transport across single biological membranes. [In:] Giebisch G., Tosteson D.C., Ussing H.H. (eds.) Membrane transport in biology, vol. I., Springer- Verlag, Berlin, pp. 1- 54.
Wąsik S., Arabski M., Drulis-Kawa Z., Gubernator J. (2013). Laser interferometry analysis of ciprofloxacin and ampicillin diffusion from liposomal solutions to water phase, Eur. Biophys. J., DOI 10.1007/s00249-013-0904-2
Wąsik S., Arabski M., Dworecki K.,. Kaca W., Ślęzak A. (2010.) Influence of gravitational field on substance transport in gels, J. Membr. Sci., 365, 341-346.