Promising Antifungal Potential of Selective Botanical Extracts, Fungicides and Trichoderma Isolates Against Alternaria solani

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Abstract

In vitro antifungal potential of Trichoderma isolates, selective botanical extracts and fungicides against A. solani was evaluated. Trichoderma isolates, i.e. T. harzianum, T. viride and T. hamatum, were tested for their antifungal effect by dual culture technique at 48, 96, 144 and 172 hrs. T. hamatum produced the highest inhibition of A. solani in vitro, followed by T. hazianum and T. viride after 172 hrs. Methanolic leaf extracts of Elettaria cardamomum, Syzygium aromaticum, Curcuma longa and root extract of Parthenium hysterophorus showed up to 100% inhibition of A. solani, compared to control, while methanolic stem and leaf extracts of P. hysterophorus produced up to 90% inhibition of the pathogen. In vitro, six different systemic fungicides Triger 25% EC (Tebuconazole), Solex (Carbendazim 40% + Triadimefon 10%), Dew (Difenoconazole), Amistor Top SC (Azoxystrobin + Difenoconazole), Corel 25% EC (Difenoconazole), Reflex (Difenoconazole + Propiconazole) were tested against A. solani at 5, 10 and 15 ppm concentrations after 48, 96, 144 and 172 hrs. Corel and reflex at all concentrations produced best growth inhibition of A. solani. The inhibition was maximum by all fungicides at 15 ppm after 172 hrs. All fungicides had a promising inhibitory effect on A. solani, except Solex. It can be concluded from the present investigation that a combination of these strategies can be used in integrated disease management of A. solani on potato.

Chourasiya, P.K., Lal, A.A. & Simon, S. (2013). Effect of certain fungicides and botanicals against early blight of tomato caused by Alternaria solani (Ellis and Martin) under Allahabad Utter Pradesh India conditions. Int.J.Agric.Sci.Res., 3(3):151-155.

Cowan, M.M. (1999). Plant products as antimicrobial agents. Clin.Microbiol.Rev., 12(4): 564-582.

Devi, S.S., Sreenivasulu, Y., Saritha, S., Kumar, M.R., Kumar K.P. & Sudhakar, P. (2012). Molecular diversity of native Trichoderma isolates against Fusarium oxysporum f. sp. lycopersici (Sacc.). A causal agent of Fusarium wilt in tomato (Lycopersicon esculentum Mill.). Arch. Phytopathol. Pl. Prot., 45(6): 686-698.

Doley, K. & Jite, P.K. (2012). In vitro efficacy of Trichoderma viride against Sclerotium rolfsii and Macrophomina phaseolina. Not.Sci.Biol., 4(4): 39-44.

Ellis, M.B. & Ellis, P. (1987). Microfungi on land plants. Croom Helm, Sydney, Australia, New South Wales, 289: 2-3.

Horsfield, A., Wicks, T., Davies, K., Wilson, D. & Paton, S. (2010). Effect of fungicide use strategies on the control of early blight (Alternaria solani) and potato yield. Austral.Pl.Pathol., 39(4): 368-375.

Javaid, A., Afzal, V.,Bashir, A. & Shoaib, A. (2014). In vitro screening of Trichoderma species against Macrophomina phaseolina and Fusarium oxysporum f. sp. lycopersici. Pak.J.Phytopathol., 26(1): 39-43.

Jee, H.J. & Kim, H.K. (1987). Isolation, identification and antagonism of rhizospheric antagonists to cucumber wilt pathogen Fusarium oxysporum f. sp. cucumerinum Owen. Kor.J.Pl.Pathol., 3(3): 187-197.

Koley, S. & Mahapatra, S.S. (2016). In vitro efficacy of systemic and nonsystemic chemicals on the growth inhibition of Alternaria solani causing early blight of tomato. Int.J.Sci.Rep., 5(5): 312-314.

Küçük, Ç. & Kıvanç, M. (2008). Mycoparasitism in the biological control of Gibberella zeae and Aspergillus ustus by Trichoderma harzianum strains. J.Agric.Technol., 4(2): 49-55.

Madden, L., Pennypacker, S.P. & MacNab, A.A. (1978). FAST, a forecast system Alternaria solani on tomato. Phytopathol., 68(9): 1354-1358.

Masih, H., Peter J.K. & Taripathi, P. (2014). A comparative evaluation of antifungal activity of medicinal plant extracts and chemical fungicides against four plant pathogens. Int.J.Curr.Microbiol.App.Sci., 3(5): 97-109.

Moosa, A., Farzand, A. & Anjum, M.Z. (2016). In vitro evaluation of botanical extracts and fungicides against Phytophthora blight of chili pepper (Capsicum Annuum L). Pak.J.Phytopathol., 28(2): 201-206.

Moosa, A., S.T. Sahi, I.U. Haq, A. Farzand, S.A. Khan & K. Javaid. 2017. Antagonistic potential of Trichoderma isolates and manures against Fusarium wilt of tomato. Int.J.Veg.Sci., 23(3): 207-218. https://doi.org/10.1080/19315260.2016.1232329

Morton, D.J. & Stroube, W.H. (1955). Antagonistic and stimulating effects of soil microorganisms upon Sclerotium rolfsii. Phytopathol., 45: 417-420.

Nakkeeran, S., Krishnamoorthy, A.S., Ramamoorthy, V. & Renukadevi, P. (2002). Microbial inoculants in plant disease control. J. Ecobiol., 14(2): 83-94.

Neergaard, P. (1945). Danish species of Alternaria and Stemphylium. Taxonomy, parasitism, economic significance. Einar Munksgaard, Copenhagen, 560.

Nene, Y.L. & Thapliyal, P.N. (2000). Fungicides in Plant Disease Control. 3rd Ed., Oxford IBH Publ.Co., New Delhi, India, 651.

Ngadze, E. (2014). In vitro and greenhouse evaluation of botanical extracts for antifungal activity against Phytophtora infestans. J.Biopest., 7(2): 198-203.

Ozbay, N. & Newman, S.E. (2004). Biological control with Trichoderma spp. with emphasis on Trichoderma harzianum. Pak.J.Biol.Sci., 7(4): 478-484.

Pathak, V.N. (1987). Laboratory manual of plant pathology. 2nd Ed., Oxford IBH Publ.Co., New Delhi, 23-25.

Pawar, V.C. & Thakar, V.S. (2007). Evaluation of the anti-Fusarium oxysporum f. sp. ciceri and anti- Alternaria porri effects of some essential oils. World J. Microbiol. Biotechnol., 23(8): 1099-1106.

Pscheidt, J.W. (1985). Epidemiology and control of potato early blight, caused by Alternaria solani. Ph.D. thesis, University of Wisconsin-Madison.

Raza, W., Ghazanfar, M.U., Iftikhar, Y., Ahmed, K.S., Haider N. & Rasheed M.H. (2016). Management of early blight of tomato through the use of plant extracts. Int.J.Zool.Stud., 1(5): 1-4.

Rotem, J. (1994). The genus Alternaria: biology, epidemiology and pathogenicity. Americ. Phytopathol. Soc., APS Press, Saint Paul, Minnesota, USA, 326 pp.

Sabalpara, A.N., Priya, J., Waghunde, R.R. & Pandya, J.R. (2009). Antagonism of Trichoderma against sugarcane wilt pathogen (Fusarium moniliformae). American-Eurasian J.Sust.Agric., 3(4): 637-638.

Sumana, K. & Devaki, N.S. (2012). In vitro evaluations of some bioagents against tobacco wilt pathogen. J.Biopest., 5(1): 18-22.

Tapwal, A., Singh, U., Teixeira da Silva, J.A., Singh, G., Garg S. & Kumar, R. (2011). In vitro antagonism of Trichoderma viride against five phytopathogens. Pest Technol., 5(1): 59-62.

Teng, P.S. & Bissonnette, H.L. (1985). Estimating potato yield responses for chemical control of early blight in Minnesota. Americ.Pot.J., 62(11): 595-606.

Touba, E.P., Zakaria, M. & Tahereh, E. (2012). Antifungal activity of cold hot water extracts of spices against fungal pathogens of Roselle (Hibiscus sabdariffa) in vitro. Microbial Pathogenesis, 52(2): 125-129. https://doi.org/10.1016/j.micpath.2011.11.001

Van der Waals, J.E., Korsten, L. & Aveling, T.A.S. (2001). A review of early blight of potatoes. Afric. Pl. Prot., 7(2): 91-102.

Vincent, J.M. (1947). Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 156:850. doi: 10.1038/159850b0

Wang, H.-C., Zou, M.-G., Zhang, Y.-J., Chen, C.-J. & Wang, J.-X., (2007). Fungicidal activity of tebuconazole against Rhizoctonia solani and its application to rice. Chin. J.Pest.Sci., 9(4): 357-362.

Whipps, J.M. & Lumsden, R.D. (2001). Commercial use of fungi as plant disease biological control agents: status and prospects. Fungal biocontrol agents: progress, problems and potential, pp. 9-22.

Xiu-Rong, Y., Shu-Gin, S. & Tao, T. (2011). Preliminary study on synergy control effect of biocontrol bacteria B579 and carbendazim on Rhizoctonia solani. J. Shandong Agric.Sci., 11: 026.

Cercetari Agronomice in Moldova

The Journal of “Ion Ionescu De La Brad” University of Agricultural Sciences And Veterinary Medicine of Iasi

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