Role of insulin during the development of oligofructose (OF)-induced equine laminitis

Open access


Horses (n = 20) were divided into 2 groups: oligofructose (OF)-induced equine laminitis group (group OF; n = 11) which received 10 g/kg b.w. of OF dissolved in 4 L water via nasogastric intubation, and control group (NS; n = 9) which received 4 L of saline. Blood was collected at 4 h intervals over 72 h study period and analysed by ELISA, kinetic limulus amoebocyte lysate assay, and glucose-oxidase methods. The level of insulin changed significantly in horses which received OF (P < 0.01); there was a significant negative correlation between the level of adiponectin and insulin over time. The results suggested that insulin may play an important role in the development of OF-induced equine laminitis by altering the level of endothelin-1 and nitric oxide.

1. Belknap J.K., Black S.J.: Sepsis-related laminitis. Equine Vet J 2012, 44, 738-740.

2. Boehm B.O., Home P.D., Behrend C., Kamp N.M., Lindholm A.: Premixed insulin aspart 30 vs. premixed human insulin 30/70 twice daily: a randomized trial in type 1 and type 2 diabetic patients. Diabet Med 2002, 19, 393-399.

3. Cavaghan M.K., Ehrmann D.A., Polonsky K.S.: Interactions between insulin resistance and insulin secretion in the development of glucose intolerance. J Clin Invest 2000, 106, 329-333.

4. Elliott J., Bailey S.R.: Gastrointestinal derived factors are potential triggers for the development of acute equine laminitis. J Nutr 2006, 136, 2103S-2107S.

5. Elliott J., Berhane Y., Bailey S.R.: Effects of monoamines formed in the cecum of horses on equine digital blood vessels and platelets. Am J Vet Res 2003, 64, 1124-1131.

6. Eps A.V., Pollitt C.C.: Equine laminitis induced with OF. Equine Vet J 2006, 38, 203-208.

7. Gauff F., Patan-Zugaj B., Licka T.F.: Hyperinsulinaemia increases vascular resistance and endothelin-1 expression in the equine digit. Equine Vet J 2013, 45, 613-8.

8. Ivy J.L.: Role of exercise training in the prevention, treatment of insulin resistance and non-insulin-dependent diabetes mellitus. Sports Med 1997, 24, 321-336.

9. Jong W.Y., Seung K.S.: The production of high-content fructooligosaccharides from sucrose by the mixed-enzyme system of fructosyltransferase and glucose oxidase. Biotechnol Lett 1993, 15, 573-576.

10. Katz L.M., Bailey S.R.: A review of recent advances and current hypotheses on the pathogenesis of acute laminitis. Equine Vet J 2012, 44, 752-761.

11. Konter J.M., Parker J.L., Baez E., Li S.Z., Ranscht B., Denzel M., Little F.F., Nakamura K., Ouchi N., Fine A., Walsh K., Summer R.S.: Adiponectin attenuates lipopolysaccharide-induced acute lung injury through suppression of endothelial cell activation. J Immunol 2012, 188, 854-863.

12. Kubota N., Terauchi Y., Yamauchi T., Kubota T., Moroi M., Matsui J., Eto K., Yamashita T., Kamon J., Satoh H., Yano W., Froguel P., Nagai R., Kimura S., Kadowaki T., Noda T.: Disruption of adiponectin causes insulin resistance and neointimal formation. J Biol Chem 2002, 277, 25863-25866.

13. Kadowaki T., Yamauchi T.: Adiponectin and adiponectin receptors. Endocrinol Rev 2005, 26, 439-451.

14. Kadowaki T., Yamauchi T., Kubota N., Hara K., Ueki K., Tobe K.: Adiponectin and adiponectin receptors in insulin resistance, diabetes, and the metabolic syndrome. J Clin Invest 2006, 116, 1784-1792.

15. Longland A.C., Byrd B.M.: Pasture nonstructural carbohydrates and equine laminitis. J Nutr 2006, 136, 2099S-2102S.

16. Maeda K.: Method of invasiveness control and use of adiponectin. WO, patent 2005/0941882 A2, 13, 10, 2005.

17. Milinovich G.J., Klieve A.V., Pollitt C.C., Trott D.J.: Microbial events in the hindgut during carbohydrate-induced equine laminitis. Vet Clin North Am Equine Pract 2010, 26, 79-94.

18. Menzies-Gow N.J., Bailey S.R., Katz L.M., Marr C.M., Elliott J.: Endotoxin-induced digital vasoconstriction in horses: associated changes in plasma concentrations of vasoconstrictor mediators. Equine Vet J 2004, 36, 273-278.

19. Neumeier M., Weigert J., Schäffler A., Wehrwein G., Müller-Ladner U., Schölmerich J., Wrede C., Buechler C.: Different effects of adiponectin isoforms in human monocytic cells. J Leukoc Biol 2006, 79, 803-808.

20. Onishi J.C., Park J.W., Prado J., Eades S.C., Mirza M.H., Fugaro M.N., Häggblom M.M., Reinemeyer C.R.: Intestinal bacterial overgrowth includes potential pathogens in the carbohydrate overload models of equine acute laminitis. Vet Microbiol 2012, 159, 354-363.

21. Pandey G., Makhija E., George N., Chakravarti B., Godbole M.M., Ecelbarger C.M., Tiwari S.: Insulin regulates nitric oxide production in the kidney collecting duct cells. J Biol Chem 2014, 290, 82-91.

22. Peter H., Chris P., Catherine M.: Advances in Equine Nutrition IV. Nottingham University Press, 2009, 136-138.

23. Pita J., Panadero A., Soriano-Guillén L., Rodríguez E., Rovira A.: The insulin sensitizing effects of PPAR-γ agonist are associated to changes in adiponectin index and adiponectin receptors in Zucker fatty rats. Regul Pept 2012, 174, 18-25.

24. Rashid P.A., Whitehurst A., Lawson N., Bath P.M.: Plasma nitric oxide (nitrate/nitrite) levels in acute stroke and their relationship with severity and outcome. J Stroke Cerebrovasc Dis 2003, 12, 82-87.

25. Shulman G.I.: Cellular mechanisms of insulin resistance. J Clin Invest 2000, 106, 171-176.

26. Snyder S.H., Bredt D.S.: Biological roles of nitric oxide. Sci Am 1992, 266, 68-71, 74-7.

27. Toth F., Frank N., Chameroy K.A. Boston R.C.: Effects of endotoxaemia and carbohydrate overload on glucose and insulin dynamics and the development of laminitis in horses. Equine Vet J 2009, 41, 852-858.

28. Treiber K.H., Kronfeld D.S., Geor R.J.: Insulin resistance in equids: possible role in laminitis. J Nutr 2006, 136, 2094S-2098S.

29. Yamauchi T., Kamon J., Waki H., Terauchi Y., Kubota N., Hara K., Kadowaki T.: The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med 2001, 7, 941-946.

30. Ziemke F., Mantzoros C.S.: Adiponectin in insulin resistance: lessons from translational research. Am J Vet Res 2010, 91, 258S-261S.

Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

Journal Information

IMPACT FACTOR 2017: 0.811
5-year IMPACT FACTOR: 0.973

CiteScore 2017: 0.68

SCImago Journal Rank (SJR) 2017: 0.287
Source Normalized Impact per Paper (SNIP) 2017: 0.484


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 249 244 23
PDF Downloads 91 87 11