The investigations comprised 100 piglets of crossbreed Polish Landrace x Large White Polish breed. Faeces samples were collected on the 2nd d of piglets’ life (control). On the 5th d of life of the piglets, probiotic paste was applied and 7 d later, faecal samples were collected again. The material included 100 isolates of Campylobacter sp. obtained from healthy piglets. All isolates were assigned to the Campylobacter coli species. The occurrence of virulence genes was determined by the PCR method. Drugresistance of the obtained isolates was determined using diffusion tests and E-test strips. All isolates deriving from the control group piglets were found to contain the cadF gene responsible for adhesion, as well as, gene flaA influencing motility of the examined bacteria. In piglets fed diets supplemented with probiotics, the cadF gene occurred in 100% isolates and gene flaA - in 99% isolates. Campylobacter coli isolates obtained from piglets from the control group exhibited the highest resistance with respect to ciprofloxacin and enrofloxacin. The similar results were recorded in the case of isolates obtained after the probiotic application. The majority of the isolates generated α type haemolysis (91%-92%). No significant differences were recorded in the capability of generating haemolysis between isolates obtained before probiotic administration and the isolates obtained after the application of the experimental probiotic.
1. Andrzejewska M., Klawe J.J., Szczepańska B., Śpica D.: Occurrence of virulence genes among Campylobacterjejuni and Campylobacter coli isolates from domestic animals and children. Polish J Vet Sci 2011, 2, 207-211.
2. Bang D.D., Scheutz F., Ahrens P., Pedersen K., Blom J., Madsen M.: Prevalence of cytolethal distending toxin (cdt) genes and CDT production in Campylobacter spp. isolated from Danish broilers. J Med Microbiol 2001, 50, 1087-1094.
4. European Food Safety Authority and European Centre for Disease Prevention and Control; The European Union Summary Report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2010. EFSA J 2012, 10, 233.
5. Istivan T.S, Smith S.C., Fry B.N., Coloe P.J.: Characterization of Campylobacter concisus hemolysins. FEMS Immunol Med Microbiol 2008, 54, 224-235.
6. Konkel M., Gray S.A., Kim B.J., Gravis S.G., Yoon J.: Identification of enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product. J Clin Microbiol 1999, 37, 510-517.
7. Krutkiewicz A.: Campylobacteriosis in humans and animals. Życie Wet 2008, 83, 285-288.
8. Lara-Tejero M., Galan J.E.: CdtA, cdtB and cdtC form a tripartite complex that is required for cytolethal distending toxin activity. Infect Immun 2001, 69, 4358-4365.
9. Lin J.: Novel approaches for Campylobacter control in poultry. Foodborne Pathog Dis 2009, 6, 755-765.
10. Murat Y., Ersin I., Burcu A., Nilgun U.: Comparison of disc diffusion and E test® for in vitro antimicrobial susceptibility testing of Campylobacter jejuni and Campylobacter coli isolated from poultry. Revue Méd Vét 2005, 156, 510-513.
11. Nachamkin I., Bohachic K., Patton C.M.: Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J Clin Microbiol 1993, 31, 1531-1536.
12. National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals: Approved Standard-Second Edition 2002, M31- A2. NCCLS, Wayne, USA.
13. Pejsak Z., Truszczyński M.: Diarrheal diseases in swine. Życie Wet 2007, 82, 108-110.
14. Rizal A., Kumar A., Vidyarthi A.S.: Prevalence of pathogenic genes in Campylobacter jejuni isolated from poultry and human. Int J Food Safety 2010, 12, 1531-1536.
15. Rzedzicki J., Kołodziejczyk A.: Birds as a potential source of human infection by Campylobacter sp. Medycyna Wet 1998, 54, 511-514.
16. SAS User’s quide. Statistics version. 1999, SAS Inst Inc Cary NC.
17. Tambur Z.Ž., Stojanov I.M., Konstantinonic S.M., Jovanowic D.V., Cenic-Miloševic D., Opačic D.N.: Multi drug resistance of Campylobacter jejuni and Campylobacter coli to tested antibiotics in strains originating from humans, poultry and swine. Matica Srpska Proceed Natural Sci 2010, 118, 27-37.
18. Ziprin R.L., Young C.R., Stanker L.H., Hume M.E., Konkel M.E.: The absence of cecal colonization of chicks by mutant of Campylobacter jejuni not expressing bacterial fibronectin-binding protein. Avian Dis 1999, 43, 586-589.