Lab-on-a-chip fluorescence detection with image sensor and software-based image conditioning

Open access

Lab-on-a-chip fluorescence detection with image sensor and software-based image conditioning

The fluorometric detection in lab-on-a-chip devices for life-sciences is one of the main detection methods. Well know from "large-scale" analytical chemistry methodologies and instrumentation has been applied in lab-on-a-chip solutions. In most cases, optical instrumentation for fluorescence induction and detection is based on configuration and components borrowed from large laboratory instruments. As a result, optical instrumentation surrounding lab-on-a-chip is bulky, expensive and dedicated for operation only inside laboratory. In this paper a discussion on fluorescence detection in lab-on-a-chip is carried out. A novel image sensor - based detection instrumentation co-working with a "clever" software is described. Instrumentation is dedicated for operation in portable and low-cost devices for different life-science applications. Examples of applications of the novel method and instrumentation co-working with various lab-on-a-chips are presented on the base of author's works.

A. Manz, N. Graber, and H.M. Widmer, "Miniaturized total chemical analysis systems: a novel concept for chemical sensing", Sensors and Actuators B1, 244-248 (1990).

A. Griebel, S. Rund, F. Schönfeld, W. Dörner, R. Konrad, and S. Hardt, "Integrated polymer chip for two-dimensional capillary gel electrophoresis", Lab Chip 4, 18-23 (2004).

M. Agirregabiria, D. Verdoy, G. Olabarria, J. Berganzo, J. Berganza, L.J. Fernandez, M. Pascual de Zulutea, K. Mayora, P. Aldamiz-Echevaria, and J.M. Ruano-Lopez, "Concentration, lysis and real-time PCR on a SU-8 lab on a chip for rapid detection of Salmonella spp. in faeces", Proc. μTAS 2007 1, 584-586 (2007).

Q. Xiang, B. Xu, R. Fu, and D. Li, "Real time PCR on disposable PDMS chip with a miniaturized thermal cycler", Biomedical Microdevices 7 (4), 273-279 (2005).

P. Neuzil, Ch. Zhang, J. Pipper, S. Oh, and L. Zhuo, "Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes", Nucleid Acid Research 34 (11), 77-86 (2006).

N. C. Cady, S. Stelick, M.V. Kunnavakkam, and C.A. Batt, "Real-time PCR detection of Listeria monocytogens using integrated microfluidic platforms", Sensors and Actuators B 107, 332-341 (2005).

Q. Xiang, B. Xu, and D. Li, "Miniature real time PCR on chip with multi-channel fiber optical fluorescence detection module", Biomedical Microdevices 9, 443-449 (2007).

D.-S. Lee, M.-H. Wu, U. Remesh, Ch.-W. Lin, T.-M.-Lee, and P.-H. Chen, "A novel real-time PCR machine with a miniature spectrometer for fluorescence sensing in a micro liter volume glass capillary", Sensors and Actuators B 100, 401-410 (2004).

L. Novak, P. Neuzil, J. Pipper, and Ch. Zhang, "A palm-sized real-time (RT) PCR", Proc. μTAS 2006 1, 332-334 (2006).

Y.-K. Cho, J. Kim, Y. Lee, Y.-A. Kim, K. Namkoong, H. Lim, K.W. Oh, S. Kim, J. Han, Ch. Park, Y.E. Pak, Ch.-S. Ki, J.R. Choi, H.-K. Myeong, and Ch. Ko, "Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus", Biosensors and Bioelectronics 21, 2161-2169 (2006).

Z. Wang, A. Sekulovic, J.P. Kutter, D.D. Bang, and A. Wolf, "Real-time PCR using a PCR microchip with integrated thermal system and polymer waveguide for the detection of Campylobacter Jejuni", Proc. MEMS 2006 1, 542-545 (2006).

R. Walczak, P. Bembnowicz, P. Szcepańska, J.A. Dziuban, L. Golonka, J. Koszur, and D.D. Bang, "Miniaturized system for real-time PCR in low-cost disposable LTCC chip with integrated optical waveguide", Proc. μTAS 2008 1, 1078-1080 (2008).

J.A. Dziuban, Bonding in Microsystem Technology, Springer, New York, 2006.

D. Augustyniak, J. Bar, A. Chełmońska-Soyta, D. Dłubek, M. Ferens-Sieczkowska, A. Jankowski, I. Katnik-Prastwoska, B. Krotkiewska, H. Krotkiewski, G. Majkowska-Skrobek, M. Orczyk-Pawiłowicz, M. Pupek, and R. Walczak, Immunochemistry in Medical Biology, PWN, Warszawa, 2009.

A. Piruska, I. Nikcevic, S. H. Lee, Ch. Ahn, W.R. Heineman, P.A. Limbach, and C.J. Seliskar, "The autofluorescence of plastic materials and chips measured under laser irradiation", Lab Chip 5, 1348-1354 (2005).

P. Szczepańska, R. Walczak, A. Wolff, and J. Dziuban, "SU-8 planar waveguides for real-time PCR chips", Proc. Eur. 2008 Conf. 1, 383-386 (2008).

J.M. Ruano-Lopez, M. Agirregabirra, G. Olabarria, D. Verdoy, D.D. Bang, M. Bu, A. Wolff, A. Voigt, J. Dziuban, R. Walczak, and J. Berganzo, "The SmartBioPhone, a point of care vision under development trough two European projects: OPTOLABCARD and LABONFOIL", Lab Chip 9 (11), 1495-1499 (2009).

M. Agirregabiria, M. Tijero, J. Berganzo, L.J. Fernandez, K. Mayora, and J.M. Ruoano-Lopez, "A smart system based on lab on a card for detection of Salmonella spp. in faeces", Proc. Smart Systems Integration 2008 1, CD ROM (2008).

D.D. Bang, J. Høgberg, M. Agirregabiria, J. Berganzo, K. Mayora, R. Walczak, J.A. Dziuban, M. Bu, A. Wolff, and J.M. Ruano-Lopez, "A total integrated lab-on-a-chip systems for rapid detection of Campylobacter spp. in broiler chicken, validation trial", Proc. μTAS 2009 1, 345-348 (2009).

R. Walczak J. Dziuban, J. Koszur, D.D. Bang, and J.M. Ruoano-Lopez, "Miniature system for real-time PCR for low-cost and mass-scale detection of food pathogens", Electronics 49 (6), 242-244 (2008), (in Polish).

R. Walczak, "Lab-on-a-chip - based DNA/PCR analyzers with fluorometric detection", PAK 56 (7), 805-808 (2010).

W. Kubicki, R. Walczak, and J. Dziuban, "Labs-on-a-chip for DNA analyze by capillary gel electrophoresis", Proc. II National Conf. on Nano and Micromechanics 1, 133 (2010).

F. von Heeren, E. Verpoorte, A. Man, and W. Thromann, "Characterization of electrophoretic sample injection and separation in a gel-filled cyclic planar microstructure", J. Microcolumn Separations 8 (6), 373-381 (1996).

S. Bargiel, R. Walczak, A. Górecka-Drzazga, and J. Dziuban, "Microcytometer with simultaneous photometric and fluorometric characterization of biosamples", Proc. Eurosensors 2008 Conf. 1, 517-519 (2008).

P. Szczepańska, R. Walczak, J. Dziuban, B. Kempisty, M. Jackowska, P. Antosik, J. Jaśkowski, and A. Chełmońska-Soyta, "Quality and viability assessment of oocytes/embryos of animals by optical characterization in lab-on-a-chip device", Proc. μTAS 2010 1, 697-698 (2010).

R. Walczak, J. Dziuban, M. Scholles, and J. Krüger, "Toward portable instrumentation for quantitative cocaine detection with lab-on-a-paper and hybrid optical readout", Procedia Chemistry 1 (1), 999-1002 (2009).

R. Walczak, J. Dziuban, and J. Krüger, "Fluorometric detector of cocaine in human sweat - portable new generation narcometer", PAK 56 (7), 812-815 (2010).

Bulletin of the Polish Academy of Sciences Technical Sciences

The Journal of Polish Academy of Sciences

Journal Information

IMPACT FACTOR 2016: 1.156
5-year IMPACT FACTOR: 1.238

CiteScore 2016: 1.50

SCImago Journal Rank (SJR) 2016: 0.457
Source Normalized Impact per Paper (SNIP) 2016: 1.239


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 113 113 8
PDF Downloads 29 29 2