Variability of silver fir (Abies alba Mill.) progeny from the Tisovik Reserve expressed in needle traits and chloroplast microsatellite DNA

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Abstract

Progeny from nineteen family lines of silver fir (Abies alba Mill.) from the Tisovik Reserve growing in an experimental plot were analyzed based on 4 chloroplast microsatellite DNA loci and 12 morphological and anatomical needle traits. The Tisovik Reserve is located in Białowieża Primeval Forest, 120 km north of the natural range limit of this species, and embraces a small and isolated natural population of silver fir. The aim of this study was to determine genetic variation within and between progeny lines. Analysis of phenotypic variation showed that the traits which differed most among individuals were the needle width and the distance from resin canals to vascular bundle. Those traits, which differed most between the progeny lines, were the number of endodermic cells around the vascular bund and the weight of hypodermic cells. In Tisovik progeny, we detected 107 different haplotypes. In progeny lines, we detected more haplotypes than in maternal trees, and most haplotypes did not exist in maternal trees. This may be the result of pollen influx from other silver fir stands. Progeny from Tisovik showed a higher level of variability in comparison with maternal trees.

Błocka A. & Staszewski T. 2007. Fluctuating asymmetry of needles as a non-specific stress indicator of Scots Pine (Pinus sylvestris L.). Forest Research Papers 4: 125-131 (in Polish).

Clark C. M., Wentworth T. R. & O’malley D. M. 2000. Genetic Discontinuity Revealed by Chloroplast Microsatellites in Eastern North American Abies (Pinaceae). Am. J. Bot. 87: 774-782.

Chudzińska E., Pawlaczyk E. M., Celiński K. & Diatta J. B. 2014. Response of Scots pine (Pinus sylvestris L.) to stress induced by different types of pollutants - testing the fluctuating asymmetry. Water Environ J. 28(4): 533-539.

Chudzińska E., Celiński K., Pawlaczyk E. M, Wojnicka-Półtorak A. & Diatta J. B. 2016. Trace element contamination differentiates the natural population of Scots pine: evidence from DNA microsatellites and needle morphology. Environ. Sci. Pollut. R. 23(21): 22151-22162.

Doyle J. J. & Doyle J. L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15.

Dzialuk A., Muchewicz E., Boratyński A., Montserrat, Boratyńska K. & Burczyk J. 2009. Genetic variation of Pinus uncinata (Pinaceae) in the Pyrenees determined with cpSSR markers. Plant Syst. Evol. 277: 197-205.

Echt C. S., Deverno L. L., Anzidei M. & Vendramin G. G. 1998. Chloroplast Microsatellites Reveal Population Genetic Diversity in Red Pine, Pinus resinosa Ait Mol. Ecol. 7: 307-316.

Excoffier L., Laval G. & Schneider S. 2005. Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1: 47-50.

Goncharenko G. G. & Savitsky B. P. 2000. Population and genetic resources of silver fir in Belarus. 121 pp. National Academy of Science of Belarus, Forest Institute, Gomel.

Gömöry D., Longauer R., Liepelt S., Ballian D., Brus R., Kraigher H., Parpan V. I., Parpan T. V., Paule L., Stupar V. & Ziegenhagen B. 2004. Variation patterns of mitochondrial DNA of Abies alba Mill. in suture zones of postglacial migration in Europe. Acta Soc. Bot. Pol. 73: 203-206.

Gostyńska-Jakuszewska M. 1972. European silver fir (Abies alba Mill.) In: K. Browicz (ed.). Atlas of species distribution of trees and shrubs in Poland, 12, pp. 5-10. Polish Scientific Publishers PWN, Warszawa-Poznań (in Polish).

Górski S. 1829. O roślinach żubrom upodobanych, jako też innych w puszczy Białowiezkiey. Dziennik Wileński 9: 207-217.

Innes J. L. & Skelly J. M. 2002. Forest decline and air pollution: an assessment of ‘forest health’ in the forests of Europe, the Northeastern United States, and Southeastern Canada. In: J. N. B. Bell & M. Treshow (eds.). Air pollution and plant life. 2nd edition, pp. 273-294. John Wiley, London.

Klánowá J., Čupr P., Baráková D., Šeda Z., Anděl P. & Holoubek I. 2009. Can pine needles indicate trends in the air pollution levels at remote sites? Environ. Pollut. 157: 3248-3254.

Konnert M. & Bergmann F. 1995. The geographical distribution of genetic variation of silver fir (Abies alba, Pinaceae) in relation to its migration history. Plant Syst. Evol. 195: 19-30.

Korczyk A. F. 1999. Estimation of genetic and breeding value of natural populations of silver fir (Abies alba Mill.) from the eastern species range in Poland. Publishing House of the University of Agriculture in Krakow. Scientific Papers 61: 155-170 (in Polish).

Korczyk A. F. 2015a. The “Tisovik" reserve of Silver fir in the Belarusian national park “Belavezhskaya Pushcha". Forest Research Papers 76(1): 18-36.

Korczyk A. F. 2015b. The ancestral conservative tillage of silver fir in the “Tisovik” reserve of the Białowieża Primeval Forest. Forest Research Papers 76(2): 153-167.

Korczyk A. F., Kawecka A., Martysevič V. V. & Strelkov A. Z. 1997. Natural stand of silver fir (Abies alba Mill.) in Białowieża Primeval Forest Papers of Forest Research Institute series A, 837: 27-62 (in Polish).

Kozlov M. V. & Niemelä P. 1999. Difference in needle length - a new and objective indicator of pollution impact on Scots pine (Pinus sylvestris). Water Air Soil Poll. 116: 365-370.

Kral F. 1980. Waldgeschichtliche Grundlagen für die Ausscheidung von Ökotypen bei Abies alba. Proceedings 3. 158-168 pp. IUFRO Tannensymposium Wien. Österr. Agrar-Verlag, Vienna.

Liepelt S., Bialozyt R. & Ziegenhagen B. 2002. Winddispered pollen mediates postglacial gene flow among refugia. PNAS USA 99: 14590-14594.

Liepelt S., Cheddadi R., De Beaulieu J.-L., Fady B., Gömöry D., Hussendörfer E., Konnert M., Litt T., Longauer R., Terhürne-Berson R. & Ziegenhagen B. 2009. Postglacial range expansion and its genetic imprints in Abies alba (Mill.) - A synthesis from paleobotanic and genetic data. Rev. Palaeobot. Palyno. 153: 139-149.

Mejnartowicz L. 1996. Origin of silver fir stands in Białowieża Primeval Forest. In: P. Paschaslis & S. Zajączkowski (eds.). Biodiversity Protection of Białowieża Primeval Forest, pp. 35-50. Warszawa.

Morgante M., Felice N. & Vendramin G. G. 1997. Analysis of Hypervariable Chloroplast Microsatellite in Pinus halepensis Reveal a Dramatic Bottleneck. In: A. Karp, P. G. Isaac & D. S. Ingram (eds.). Molecular Tools for Screening Biodiversity: Plants and Animals, pp. 402-412. Chapman and Hall, London.

Neale D. B. & Sederoff R. R. 1989. Paternal Inheritance of Chloroplast DNA and Maternal Inheritance of Mitochondrial DNA in Loblolly Pine. Theor. Appl. Genet. 77: 212-216.

Nei M. 1972. Genetic distance between populations. Amer. Natur. 106: 283-292.

Parfenov V. I. & Savchuk S. S. 2015. Abies alba Mill. In: I. M. Kachanovskiy, M. Ye. Nikiforov, V. I. Parfenov, O. I. Borodin, A. V. Pugachevskiy, V. M. Baychorov, O. S. Gapiyenko, A. S. Giryachev & T. P. Yevdaseva (eds.). 2015. Krasnaya kniga Respubliki Belarus, rasteniya: redkiye i nakhodyashchiyesya pod ugrozoy ischeznoveniya vidy dikorastushchikh rasteniy, 4. ed., 25-26 pp. Belaruskaya Entsyklapedyya іmya P. Broўkі, Minsk, (in Russian).

Pawlaczyk E. M. & Bobowicz M. A. 2008a. Silver fir (Abiesalba Mill.) differentiation from Jata Reserve as expressedby morphological and anatomical features of needles Forest Research Papers 69: 243-253 (in Polish).

Pawlaczyk E. M. & Bobowicz M. A. 2008b. Międzyrodowe zróżnicowanie jodły pospolitej (Abies alba Mill.) z Rezerwatu “Kamienna Góra” z Roztoczańskiego Parku Narodowego z uprawy doświadczalnej wyrażone w cechach morfologii i anatomii igieł. In: K. Kannenberg & H. Szramka (eds.). Management of Environmental Protection in Forests, 2, pp. 38-46. Higher School of Environmental Management, Tuchola.

Pawlaczyk E. M., Bobowicz M. A. & Korczyk A. F. 2002. Variability of three populations of Abies alba Mill. expressed in morphological and anatomical needle traits. Ecological Questions 2: 25-32.

Pawlaczyk E. M., Bobowicz M. A. & Lesiczka P. 2010. Genetic differentiation of four progeny of silver fir (Abies alba Mill.) from Tisovik Reserve from experimental culture in Hajnówka District expressed in morphology and anatomy of needles traits. Management of Environmental Protection in Forests 4: 120-138 (in Polish).

Pawlaczyk E. M., Grzebyta J., Bobowicz M. A. & Korczyk A. F. 2005. Individual differentiation of Abies alba Mill. population from the “Tisovik” Reserve. Variability expressed in morphology and anatomy of needles. Acta Biol. Cracov. Bot. 47: 137-144.

Pawlaczyk E. M., Kroplewska I. & Bobowicz M. A. 2013. Postglacial migration of silver fir (Abies alba Mill.) to Poland - analysis on the basis of mitochondrial DNA polymorphism. Sylwan 157: 458-463 (in Polish).

Pawlaczyk E. M., Bączkiewicz A., Buczkowska K. & Bobowicz M. A. 2017. Genetic variation of silver fir progeny from Tisovik Reserve population determined via microsatellite and isozyme markers. Folia For. Pol. Series A Forestry 59(1): 45-58, DOI: 10.1515/ffp-2017-00051

Peakall R. & Smouse P. E. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol. Ecol. Notes 6: 288-295.

Provan J., Soranzo N., Wilson N. J., Mcnicol J. W., Forrest G. I., Cottrell J. & Powell W. 1998. Gene-pool variation in Caledonian and European Scots pine (Pinus sylvestris L.) revealed by chloroplast simple-sequence repeats. P. Roy. Soc. Lond. B. Bio. 265: 1697-1705.

Semerikova S. A. & Semerikov V. L. 2007. The diversity of chloroplast microsatellite loci in siberian fir (Abies sibirica Ledeb.) and two far east fir species A. nephrolepis (Trautv.) Maxim. and A. sachalinensis. Russ. J. Genet. 43: 1373-1381.

Szymańska M. 2007. Genetyczna zmienność potomstwa jodły pospolitej Abies alba Mill. z rezerwatu Tisovik w uprawie testowej oszacowana za pomocą cech morfologii i anatomii igieł. Ph. D. Thesis, Adam Mickiewicz University, Poznań, Poland.

Vendramin G. G., Degen B., Petit R. J., Anzidei M., Madaghiele A. & Ziegenhagen B. 1999. High level of variation at Abies alba chloroplast microsatellite loci in Europe. Mol. Ecol. 8: 1117-1126.

Vendramin G. G., Lelli L., Ross i P. & Morgante M. 1996. A set of primers for the amplification of 20 chloroplast microsatellites in Pinaceae. Mol. Ecol. Notes 5: 111-114.

Vendramin G. G. & Ziegenhagen B. 1997. Characterization and inheritance of polymorphic plastid microsatellites in Abies. Genome 40: 857-864.

Wright J. W. 1976. Introduction to Forest Genetics. 463 pp. Academic Press, New York.

Yeh F. C., Yang R. & Boyle T. 1999. PopGen 1.0. http://cc.oulu.fi/~jaspi/popgen/popgen.htm

Zając A. & Zając M. (eds.). 2001. Distribution Atlas of Vascular Plants in Poland. xii+714 pp. Edited by Laboratory of Computer Chorology, Institute of Botany, Jagiellonian University, Cracow.

Ziegenhagen B., Fady B., Kuhlenkamp V. & Liepelt S. 2005. Differentiating groups of Abies species with a simple molecular marker. Silvae Genet. 54: 123-126.

Ziegenhagen B., Scholz F., Madaghiele A. & Vendramin G. G. 1998. Hypervariable chloroplast microsatellites as markers for paternity analysis in Abies alba. Can. J. Forest. Res. 28: 317-321.

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