Effect of Fe3O4 and TiO2 Nanoparticles on Catalase Activity and β-Carotene Content at Pigmented Yeast Strain Rhodotorula gracilis

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The properties of nanoparticles have been used in a wide range of potential applications in food industry, medicine, microbial biotechnology, cosmetics, environmental production. Research results of evaluation of the effect of Fe3O4 and TiO2 nanoparticles applicated in large concentration limits on some parameters of pigmented yeast strain Rhodotorula gracilis CNMN-Y-30 are presented in this paper. It was established that nanoparticles selected for study caused toxic effects on β-caroten accumulation and activity of antioxidant enzyme catalase depending on concentration and nanostructure. The strong correlation between concentration of nanoparticles and evaluated components has been revealed at studied yeast strain. Coefficients of correlation varied in limits R2 = 0.623... 0.951. For the first time, the determination of β-carotene accumulation and catalase activity rate as functional tests for estimation of toxicity of nanoparticles for pigmented yeast strain Rhodotorula gracilis was effectuated. Thus, it was established that adaptive response of yeast strain to the presence of Fe3O4 and TiO2 nanoparticles has been manifested by modification of the processes of β-carotene biosythesis and catalase activity.

1. Aguilar-Uscanga, B., Francois, J. (2003). A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation. Letters in Applied Microbiology, 37, 268-274.

2. Beşliu, A., Chiriţa, E., Usatîi, A. (2016). Influence nanoparticles Fe3O4 and TiO2 on growth and biochemical composition of yeast strain Rhodotorula gracilis CNMN-Y-30. Studia Universitatis Moldaviae, 1 (91), 72, 1814-3237.

3. Buteică, S. (2014). Nanoparticule de oxid de fier cu înveliș polar: proprietăți și perspective biomedicale. Rezumat Teză de doctorat, Universitatea de Medicină și Farmacie din Craiova, Craiova, 11.

4. Chen, H., Seiber, J., Hotze, M. (2014). ACS Select on nanotechnology in food and agriculture: perspective on implications and applications. Journal of Agricultural and Food Chemistry. 62, 1209-1212.

5. Efremova, N., Usatîi, A., Molodoi, E. (2013). Metodă de determinare a activității catalazei. Brevet de invenţie MD 4205, MD-BOPI nr. 2/2013.

6. Mohapatra, M., Anand, S. (2010). Synthesis and applications of nanostructured iron oxides/hydroxides – a review. International Journal of Engineering, Science and Technology, 2, 8, 127-146.

7. Murray, R. (2008). Nanoelectrochemistry: metal nanoparticles, nanoelectrodes and nanopores. Chemical Reviews, 108 (7), 2688-2720, doi.10:1021/cr068077.l.

8. Otero-Gonzalez, L., Garcia-Saucedo, C., Field, G., Sierra-Alvarez, R. (2013). Toxicity of TiO2, ZrO2, FeO, Fe2O3 and Mn2O3 nanoparticles to the yeast, Saccharomyces cerevisiae. Chemosphere, 93, 1201-1206.

9. Rai, M., Duran, N. (2011). Metal Nanoparticles in Microbiology. Springer-Verlag Berlin Heidelberg, DOI 10.1007/978-3-642-18312-6_1.

10. Sastry, R., Rashmi, H., Rao, N. (2011). Nanotechnology for enhancing food security in India. Food Policy. 36, 391-400.

11. Sebaugh, J. (2011). Guidelines for accurate EC50/IC50 estimation. Pharmaceutical Statistics. Mar-Apr; 10(2):128-134. doi: 10.1002/pst.426.

12. Sparrenberger, K., Friedrich, R., Schiffner, M., Schuch, I., & Wagner, M. (2015). Ultra-processed food consumption in children from a Basic Health Unit. Jornal de Pediatria (Rio J). 91(6), 535-542, doi:10.1016/prd.2015.01 007.

13. Tamas, V., Neamțu, G. (1986). Pigmenți carotenoidici și metaboliți. Ed. Ceres, București., România, vol.1, 269.

14. Usatii, A., Kiritsa, E., Beshliu, A. (2016). The effect of Fe3O4 nanoparticles on bioproduction parameters of Rhodotorula gracilis CNMN-Y-30 yeast strain with high biotechnological potential. Analele Universităţii din Oradea, Fascicula Biologie, 2, 66-70.

15. Weir, A., Westerhott, P., Lars, F., Hristovski, K., Von Goetz, N. (2012). Titanium dioxide in food and personal Care products. Environmental Science and Technology, 6, 2242-2250.

16. Yang, Z. (2010). Review of nanoparticles functionality and toxicity on the central nervous system. Journal of the Royal Society Interface. 411-422. doi:10.1098/rsif.2010.0158.focus.

Acta Universitatis Cibiniensis. Series E: Food Technology

The Journal of „Lucian Blaga“ University of Sibiu

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