HPLC-DAD profiling and antioxidant activity of the n-butanol extract from aerial parts of Algerian Crithmum maritimum L.

Open access

Abstract

In this study, phenolic compounds from Crithmum maritimum L. n-butanol soluble fraction were quantified and identified spectrophotometrically and by using HPLC-DAD technics. They equally investigated for their antioxidant potential utilizing six in vitro assays: DPPH, ABTS•+, O2•−, Bleaching of β-carotene in linoleic acid, CUPRAC and Ferric reducing power. High amounts of total phenolics and flavonoids were recorded: 161.57± 0.479 μg GAeq.mg−1 and 31.56± 0.291 μg Qeq.mg−1 respectively. Nine compounds among them hydroxicinnamic acid and hydroxybenzoic acid derivatives, coumarins and flavonoids were identified. Chlorogenic acid known for their various pharmacological properties was detected as major compound of the extract. Rutin, vanillin, trans-2-hydroxycinnamic acid, ellagic acid, ferrulic acid, 6,7 dihydroxy coumarin, methyl 1,4 benzoquinone and trans-cinnamic acid were also detected. The extract was found to exhibit strong antioxidant capacities in all systems. Based on these results, it is right to conclude the n-butanol extract is promising source of natural antioxidants.

[1] Aruoma, O. I., Free radicals, oxidative stress, and antioxidants in human health and disease, J. Am. Oil Chem. Soc. 1998, 75, 199–212.

[2] Montaigner, L.; Olivier, R.; Pasquier, C., Oxidative stress in Cancer. AIDS and neurodegenerative deseases, Eds., Marcel Dekker, New York, 157, 1998.

[3] Riboli, E.; Norat, T., Epidemiologic evidence of the protective effect of fruit and vegetables on cancer risk, Am. J. Clin. Nutr. 2003, 78, 559–569.

[4] Letelier, M.E.; Molina-Berríos, A.; Cortés-Troncoso, J.; Jara-Sandoval, J.; Holst, M.; Palma, K.; Montoya, M.; González-Lira, V., DPPH and oxygen free radicals as pro-oxidant of biomolecules, Toxicol. Vitro. 2008, 22, 279–286.

[5] Li, A. N.; Li, S.; Li, H. B.; Xu, D. P.; Xu, X. R.; Chen, F., Total phenolic contents and antioxidant capacities of 51 edible and wild flowers, J. Funct. Foods, 2014, 6, 319–330.

[6] Dos Santos, M.; Almeida, MC.; Lopes, N. P.; De Souza, G. E. P., Evaluation of the Anti inflammatory, Analgesic and Antipyretic Activities of the Natural Polyphenol Chlorogenic Acid, Biol. Pharm. Bull. 2006, 29, 2236-2240.

[7] Cheng, J.; Dai, F.; Zhou, B.; Yang, L.; Liu, Z., Antioxidant activity of hydroxycinnamic acid derivatives in human low density lipoprotein: mechanism and structure-activity relationship, Food Chem. 2007, 104, 132–139.

[8] Raffa, D.; Maggio, B.; Raimondi, M. V.; Plescia, F.; Daidone, G., Recent discoveries of anticancer flavonoids, Eur. J. Med. Chem. 2017, 142, 213-228.

[9] Coiffard, L., Aperçu historique sur l’utilisation médicinale et alimentaire du crithme maritime, Revue d’histoire Pharmaceutique, 1991, 38, 313-317.

[10] Renna, M.; Gonnella, M., The use of the sea fennel as a new spice-colorant in culinary preparations, Int. J. Gastron. Food Sci. 2012, 1, 111-115.

[11] Males, Z.; Blazevic, N.; Plazibat, M., Variations in the yield and composition of the essential oil of Crithmum maritimum L., Acta. Pharm. 2001, 51, 81–84.

[12] Quezel P.; Santa, S., Nouvelle flore de l’Algérie et des régions désertiques méridionales, Eds. C.N.R.S, Paris, 1963.

[13] Meot-Duros, L.; Le Floch, G.; Magne, C., Radical scavenging, antioxidant and antimicrobial activities of halophytic species, J. Ethnopharmacol. 2008, 116, 258–262.

[14] Nabet, N.; Boudries, H.; Chougui, N.; Loupassaki, S.; Souagui, S.; Burló, F.; Hernández, F.; Carbonell-Barrachina, A. A.; Madani K.; Larbat, R., Biological activities and secondary compound composition from Crithmum maritimum aerial parts, Int. J. Food Prop. 2017, 20, 1843-1855.

[15] Generalic Mekinic, I.; Blazevic, I.; Mudnic, I.; Burcul, F.; Grga, M.; Skroza, D.; Jercic, I.; Ljubenkov, I.; Boban, M.; Miloš, M.; Katalinic, V., Sea fennel (Crithmum maritimum L.): phytochemical profile, antioxidative, cholinesterase inhibitory and vasodilatory activity, J. Food Sci. Technol. 2016, 53, 3104-3112.

[16] Generalić Mekinić, I.; Šimat, V.; Ljubenkov, I.; Burčul, F.; Grga, M.; Mihajlovski, M.; Lončar, R.; Katalinić, V.; Skroza, D., Influence of the vegetation period on sea fennel, Crithmum maritimum L. (Apiaceae), phenolic composition, antioxidant and anticholinesterase activities, Ind. Crop. Prod. 2018, 124, 947-953.

[17] Franke, W., Vitamin C in sea fennel (Crithmum maritimum), an edible wild plant, Econ. Bot. 1982, 36, 163-165.

[18] Coiffard L.; De Roeck-Holtzhauer, Y., Crithmum maritimum L. (Apiaceae): Hydromineral composition and phytochemical screening, Ann. Pharm. Fr. 1994, 52, 153–159.

[19] Coiffard L.; De Roeck-Holtzhauer, Y., Geographical and seasonal variations in protein content and free aminoacids of Crithmum maritimum L. (Apiaceae), Acta. Bot. Gallica, 1995, 142, 405-414.

[20] Guil-Guerrero, J. L.; Rodriguez-Garcia, I., Lipids classes, fatty acids and carotenes of the leaves of six edible wild plants, Eur. Food Res. Technol. 1999, 209, 313-316.

[21] Singleton V. L.; Rossi, J.A.J., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Amer. J. Enol. Viticult. 1965, 16,144-158.

[22] Miliauskas, G.; Venskutonis P.R.; Beek, T.A., Screening of radical scavenging activity of some medicinal and aromatic plant extracts, Food Chem. 2004, 85, 231-237.

[23] Barros, L.; Duenas, M.; Ferreira, I.C.F.R.; Baptista P.; Santos-Buelga, C., Phenolic acids determination by HPLC–DAD–ESI/MS in sixteen different Portuguese wild mushrooms species, Food Chem. Toxicol. 2009, 47, 1076-1079.

[24] Tel-Çayan, G.; Öztürk, M.; Duru, M. E.; Rehman, M. U.; Adhikari, A.; Türkoglu A.; Choudhary, M. I., Phytochemical investigation, antioxidant and anticholinesterase activities of Ganoderma adspersum, Ind. Crop. Prod. 2015, 76, 749-754.

[25] Blois, M. S., Antioxidant determinations by the use of a stable free radical, Nature, 1958, 181, 1199-1200.

[26] Re, R.; Pellegrini, A.; Proteggente, A.; Pannala, M.; Yang M.; Rice-Evans, C., Antioxidant activity aplying an improved ABTS radical cation decolorization assay, Free Radic. Biol. Med. 1999, 26, 1231-1237.

[27] Marco, G.J., A rapid method for evaluation of antioxidants, J. Am. Oil Chem. Soc. 1968, 45,594-598.

[28] Elizabeth K.; Rao, M.N.A.,Oxygen radical scavenging activity of curcumin, Int. J. Pharm. 1990, 58, 237-240.

[29] Oyaizu, M., Studies on products of browning reactions: antioxidative activities of browning reaction prepared from glucosamine, Jap. J. Nutr. 1986, 44, 307–315.

[30] Apak, R.; Güçlü, K.; Özyürek M.; Çelik, S. E., Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay, Microchim. Acta. 2008, 160, 413–419.

[31] Öztürk, M.; Kolak, U.; Topçu, G.; Öksüz S.; Choudhary M.I., Antioxidant and anticholinesterase active constituents from Micromeria cilicica by radical-scavenging activity-guided fractionation, Food Chem. 2011, 126, 31–38.

[32] Havsteen, H.B., The biochemistry and medical significance of the flavonoids, Pharmacol. Ther. 2002, 96, 67-202.

[33] Pandey, M. M.; Vijayakumar, M.; Rastogi S.; Rawat, A.K.S., Phenolic content and antioxidant properties of selected Indian spies of Apiaceae, J. Herbs Spices Med. Plants, 2012, 18, 246-256.

[34] Upadhyay R.; Mohan Rao, L., An outlook on chlorogenic acidsoccurrence, chemistry, technology, and biological activities, Crit. Rev. Food Sci. Nutr. 2013, 53, 968–984.

[35] Farah, A.; De Paulis, T.; Trugo L. C.; Martin, P. R., Effect of roasting on the formation of chlorogenic acid lactones in coffee, J. Agric. Food Chem. 2005, 53, 1505-1513.

[36] Robinson, W. E.; Reinecke, M. G.; Abdel-Malek, S.; Jia Q.; Chow, S. A., Inhibitors of HIV-1 replication that inhibit HPV integrase, Proc. Natl. Acad. Sci. USA. 1996, 93, 6326-6331.

[37] Houta, O.; Akrout, A.; Neffati M.; Amri, H., Phenolic contents, antioxidant and antimicrobial potentials of Crithmum maritimum cultivated in Tunisia arid zones, J.B.A.P.N. 2011, 1, 138-143.

[38] Jallali, I.; Zaouali, Y.; Missaoui, I.; Smeoui, A.; Abdelly C.; Ksouri, R., Variability of antioxidant and antibacterial effects of essential oils and acetonic extracts of two edible halophytes: Crithmum maritimum L. and Inula crithmoïdes L., Food Chem. 2014, 145, 1031-1038.

Journal Information

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 157 157 59
PDF Downloads 128 128 34