Anti-proliferative activity of ethyl acetate extracts of Tamarix gallica L. grown at different climatic conditions in Algeria

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Abstract

The aerial parts of T. gallica collected from three different locations (arid, humid and semi-arid) were extracted using ethyl acetate. The crude extracts were subjected to phenolic appraisal and antiproliferative activity using ELISA and xCELLigence assays. The total phenolic and flavonoids were evaluated using appropriate techniques to give a yield of total phenolics ranging between 238.46 and 348.56 mg GAE (Gallic acid equivalent)/g dry weight extract. The flavonoids yield was found to vary from 36.6 to 103.14 mg QE (quercetin equivalent)/g dry weight extract. Moreover, the extracts were tested against rat brain tumor (C6) and human cervix carcinoma (HeLa) cell lines and displayed important differences in activity. These disparities highlighted the effect of climatic factors as quality determinants of secondary metabolites and therefore as a key control of the biological therapeutic effect.

[1]. Doudach, L.; Benbacer, L.; Meddah, B.; Faouzi, M.E.A.; Hammani, K.; El Mzibri, M.; Herrah, Y., Extract of 1:5 mixture of five Moroccan medicinal plants has cytotoxic effect on some human cancer cell lines, Int. J. Pharm. 2013, 3, 301-307.

[2]. Fattahi, S.; Ardekani, A.M.; Zabihi, E.; Abedian, Z.; Mostafazadeh, A.; Pourbagher, R.; Akhavan-Niaki, H., Antioxidant and apoptotic effects of an aqueous extract of Urticadioicaon the MCF-7 human breast cancer cell line, Asian Pac. J. Cancer Prev. 2013, 14, 5317-5323

[3]. Ziech, D.; Anestopoulos, I.; Hanafi, R.; Voulgaridou, G.P.; Franco, R.; Georgakilas, A.G.; Pappa, A.; Panayiotidis, M.I., Pleiotrophic effects of natural products in ROS-induced carcinogenesis: the role of plant derived natural products in oral cancer chemoprevention, Cancer Lett. 2012, 327, 16–25.

[4]. Carmona-Ramirez, I.; Santamaria, A.; Tobon-Velasco, J.C.; Orozco-Ibarra, M.; Gonzalez-Herrera, I.G.; Pedraza-Chaverri, J.; Maldonado, P.D., Curcumin restores Nrf2 levels and prevents quinolinic acid-induced neurotoxicity, J. Nutr.Biochem. 2013, 24, 14–24.

[5]. Lefahal, M.; Benahmed, M.; Louaar, S.; Zallagui, A.; Duddeck, D.; Medjroubi, K.; Akkal, A., Antimicrobial Activity of Tamarix gallica L. Extracts and Isolated Flavonoids, Advances in Natural and Applied Sciences, 2010, 4(3), 289-292

[6]. Lefahal, M.; Zaabat, N.; Djarri, L.; Benahmed, M.; M, Kamel.; Laouer, H.; Akkal,S., Evaluation of the antioxidant activity of extracts and flavonoids obtained from Buniumalpinum Waldst. & Kit. (Apiaceae) and Tamarix gallicaL. (Tamaricaceae)Curr, Issues Pharm. Med. Sci., 2017, 30(1), 5-8.

[7]. Kendour, Z.; Ladjel, S.; Gherraf, N.; Ouahrani, M.R., Antimicrobial activity of nine medicinal plants growing in the south of Algeria, Annals of Biological Research, 2010, 1 (4): 145-14.

[8]. Tagarelli, G.; Tagarelli. A.; Piro, A., Folk medicine used to heal malaria in Calabria (southern Italy), J. Ethnobiol. Ethnomed., 2010, 6, 27-27.

[9]. Boulaaba, M.; Tsolmon, Soninkhishig.; Ksouri, R.; Han, J.; Kawada, K.; Smaoui A.; Abdelly, C.; Isoda, H., Anticancer effect of Tamarixgallica extracts on human colon cancer cells involves Erk1/2 and p38 action on G2/M cell cycle arrest, Cytotechnology. 2013, 65(6),927–936.

[10]. Singleton, V.L.; Orthofer, R.; Lamuela-Raventos, R.M., Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteureagent, Methods Enzymol. 1999, 299, 152-178.

[11]. Djeridane, A.; Yousfi, M.; Nadjemi, B.; Boutassouna, D.; Stoker, P.; Vidal, N., Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds, Food Chem.2006, 79, 654-660.

[12]. Demirtas, I.; Sahin-Yaglioglu, A., Bioactive Volatile Content of the Stem and Root of Centaureacarduiformis C. subsp. carduiformis var. carduiformis, Journal of Chemistry. 2012; doi: 10.1155/2013/125286.

[13]. Koldaş, S.; Demirtas, I.; Ozen, T.; Demirci, M.A.; Behçet, L., Phytochemical screening, anticancer and antioxidant activities of Origanum vulgare L. ssp. viride (Boiss.) Hayek, a plant of traditional usage, J. Sci. Food Agric. 2015, 95, 786-1482.

[14]. Tabet, A.; Boukhari; Abbes., Antioxidant and Antibacterial Activities of Two Algerian Halophytes, Int. J. Pharm. Sci. Rev. Res., 2018, 50(1), 114-121

[15]. Chaturvedi, S.; Drabu, S.; Sharma, M., Antioxidant activity total phenolic and flavonoid content of aerial parts of Tamarix gallica, International Journal of Phytomedicine, 2012, 4, 174-180

[16]. Isik, S.; Karagoz, A.; Karaman, S.; Nergiz, C., Proliferative and apoptotic effects of olive extracts on cell lines and healthy human cells, Food Chem., 2012, 134, 29-36.

[17]. Al-Jaber, N.; Allehaib, L., The Pharmacological Activity of Some Tamaricaceae Plants, Nat Prod Chem Res 2017, 5, 262.

[18]. Syed, A. N.; Malladi, S.R.; CH. Pradeep, K.; Suhasini, B.; Sudheer, K.D., Anti-hyperlipidemicactivity of tamarix gallica extracts in triton x-100 induced hyperlipidemic rats, International Journal Of Pharmacy & Technology, 2015, 6(4), 7880-7895

[19] Ionescu, D.; Aldea, I. M.; Bleotu, C.; Conomescu, M. C.; Matei, L.; Rizea, G. D.; Crisan, I.; Diaconu C. C.; Mihele, D., Evaluation of antiviral and cytotoxic activities of tamarix gallica and silybum marianum extracts. FARMACIA, 2014, 62(2), 329-341

[20] Ben Hmidene, A.; Hanaki, M.; Murakami, K.; Irie, K.; Isoda, H.; Shigemori, H., Inhibitory Activities of Antioxidant Flavonoids from Tamarix gallica on Amyloid Aggregation Related to Alzheimer’s and Type 2 Diabetes Diseases, Biol Pharm Bull. 2017, 40(2), 238-241 doi: 10.1248/bpb.b16-00801.

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