Impact of a polyunsaturated fatty acid supplement on enriching the nutritional value of brine shrimp nauplii, Artemia sp.

Open access


The first aim of the study was to determine the impact the commercial product S.presso (INVE Aquaculture, Belgium) had on enriching the proximate composition of brine shrimp (Artemia sp.). The second aim of the study was to develop a better enrichment protocol for preparing enriched brine shrimp larvae. Two different enrichment protocols were investigated; the supplement was administered in either one (0.5 g L-1) or two equal (0.25 g L-1) doses. Using S.presso to enrich brine shrimp nauplii with polyunsaturated fatty acids resulted in higher levels of total lipids and higher quantities of docosahexaenoic acid DHA and eicosapentaenoic acid EPA deposition. Additionally, the enrichment supplement did not lead to overall lowered protein content values caused by increased hydrolytic activity that likely facilitates digestion in larval fish as they shift to exogenous feeding.

Adloo M.N., Matinfar A., Sourinezhad I. 2012 - Effects of feeding enriched Artemia franciscana with HUFA, vitamin C and E on growth performance, survival and stress resistance of yellowfin seabream larvae - J. Aquacult. Res. Dev. 3: 157.

Akbary P., Hosseini S.A., Imanpoor M., Sudagar M., Makhdomi N.M. 2010 - Comparison between live food and artificial diet on survival rate, growth and body chemical composition of Oncorhynchuss mykiss larvae - Iran. J. Fish. Sci. 9: 19-32.

Aragăo C., Conceiçăo L.E.C., Dinis M.T., Fyhn H.J. 2004 - Amino acid pools of rotifers and Artemia under different conditions: nutritional implications for fish larvae - Aquaculture 234: 429-445.

Baidalinova L.S., Kryvych V.S., Balhodina L.P. 1977 - Methodological recommendations and guidelines for gas chromatography of fatty acids - Kaliningrad, Russia: AtlantNIRO, 33 p. (in Russian).

Bengtson D.A., Léger P., Sorgeloos P. 1991 - Use of Artemia as a food source for aquaculture - In: Artemia Biology (Eds) R.A. Broune, P. Sorgeloos, C.N.A. Trotman, CRC Press, Boca Raton, FL: 255-280.

Cahu C., Infante J.Z., Takeuchi T. 2003 - Nutritional components affecting skeletal development in fish larvae - Aquaculture 227: 245-258.

Caraway W.T. 1959 - A stable starch substrate for the determination of amylase in serum and other body fluids - Am. J. Clin. Pathol. 32: 97-99.

Chakraborty R.D., Chakraborty K., Radhakrishnan E.V. 2007 - Variation in fatty acid composition of Artemia salina nauplii enriched with microalgae and baker’s yeast for use in larviculture - J. Agric. Food Chem. 55: 4043-4051.

Chepurkina M.A., Gilyeva E.A., Prusiñska M., Kolman R. 2014 - The use of method of live feed enrichment in sturgeon aquaculture - V’estnik Rybohoz’aystvennoy Nauki 4: 78-90 (in Russian).

Dąbrowski K., Glogowski J. 1977 - Studies on the proteolytic enzymes of invertebrates constituting fish food - Hydrobiologia 52: 171-174.

Demény F., Trenovszki M.M., Sokoray-Varga S., Hegyi A., Urbányi B., ¯arski D., Ács B., Miljanoviæ B., Specziár A., Müller T. 2012 - Relative efficiencies of Artemia nauplii, dry food and mixed food in intensive rearing of larval crucian carp (Carassius carassius) - Turk. J. Fish. Aquat. Sc. 12: 691-698.

Folch J., Lees M., Stanley G.H.S. 1957 - A simple method for the isolation and purification of total lipides from animal tissues - J. Biol. Chem. 226: 497-509.

García-Ortega A., Verreth J.A.J., Coutteau P., Segner H., Huisman E.A., Sorgeloos P. 1998 - Biochemical and enzymatic characterization of decapsulated cysts and nauplii of the brine shrimp Artemia at different developmental stages - Aquaculture 161: 501-514.

Gawlicka A., Parent B., Horn M.H., Ross N., Opstad I., Torrissen O.J. 2000 - Activity of digestive enzymes in yolk-sac larvae of Atlantic halibut (Hippoglossus hippoglossus): indication of readiness for first feeding - Aquaculture 184: 303-314.

GOST 20264.2-88 1988 - Enzyme preparations. Methods for determination of proteolytic activity - Izdatelstvo standartov. Moscow, Russia, 15 p. (in Russian).

GOST R 54058-2010 2011 - Producty pyshchevye functcionalnye. Metod opredelenyja karotynoidov - Standartinform. Moscow, Russia, 8 p. (in Russian).

Hafezieh M., Kamarudin M.S., Bin Saad C.R., Abd Sattar M.K., Agh N., Hosseinpour H. 2009 - Effect of enriched Artemia urmiana on growth, survival and composition of larval Persian Sturgeon - Turk. J. Fish. Aquat. Sc. 9: 201-207.

Henderson R.J. 1996 - Fatty acid metabolism in freshwater fish with particular reference to polyunsaturated fatty acids - Arch. Anim. Nutr. 49: 5-22.

Hrytsyniak I., Smolyaninov K., Yanovich D., Vudmaska I., Yanovich V. 2009 - The biological role of ω-3 polyunsaturated fatty acids and the peculiarities of their metabolism in fresh-water fish - Fishery Science of Ukraine 1: 83-87.

ISO 2002 - Animal Feeding Stuffs: Determination of Crude Ash - International Organization for Standardization ISO 5984:2002(E), Switzerland.

Kamaszewski M., Ostaszewska T., Prusińska M., Kolman R., Chojnacki M., Zabytyvskij J., Jankowska B., Kasprzak R. 2014a - Effects of Artemia sp. enrichment with essential fatty acids on functional and morphological aspects of the digestive system in Acipenser gueldenstaedtii larvae - Turk. J. Fish. Aquat. Sci. 14: 1-10.

Kamaszewski M., Wójcik M., Ostaszewska T., Kolman R., Prusiñska M. 2014b - The effect of essential fatty acid (EFA) enrichment of Artemia sp. nauplii on the enzymatic activity of Atlantic sturgeon (Acipenser oxyrinchus Mitchill, 1815) larvae - preliminary study - J. Appl. Ichthyol. 30: 1256-1258.

Kates M. 1973 - Techniques of lipidology. Isolation, analysis and identification of lipids - American Elsevier Pub. Co., Inc., New-York, 341 p.

Kim H., Miyazaki M., Ntambi J.M. 2002 - Dietary cholesterol oppose PUFA-mediated repression of the stearoyl-CoA desaturase-1 gene by SREBP-1 independent mechanism - J. Lipid Res. 43: 1750-1752.

Knight J.A., Anderson S., Rawles J.M. 1972 - Chemical basis of the sulfo-phospho-vanillin. Reaction for estimating total serum lipid - Clin. Chem. 18: 199-202.

Kolman R., Khudyi O., Kushniryk O., Khuda L., Prusinska M., Wiszniewski G. 2018 - Influence of temperature and Artemia enriched with ω-3 PUFAs on the early ontogenesis of Atlantic sturgeon, Acipenser oxyrinchus Mitchill, 1815 - Aquac. Res. 49: 1740-1751.

Kozarenko T.D. 1975 - Ion-exchange chromatography of amino acids - Nauka, Novosibirsk, 134 p.

Lall S.P., Lewis-McCrea L.M. 2007 - Role of nutrients in skeletal metabolism and pathology in fish - an overview - Aquaculture 267: 3-19.

Léger P., Bengtson D.A., Simpson K.L., Sorgeloos P. 1986 - The use and nutritional value of Artemia as a food source- Oceanogr. Mar. Biol. Ann. Rev. 24: 521-623.

Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951 - Protein measurement with the Folin phenol reagent - J. Biol. Chem. 193: 265-275.

McEvoy L.A., Navarro J.C., Bell J.G., Sargent J.R. 1995 - Autoxidation of oil emulsions during the Artemia enrichment process - Aquaculture 134: 101-112.

Monroig O., Navarro J.C., Amat F., Gonzales P., Bermejo A., Hontoria F. 2006 - Enrichment of Artemia nauplii in essential fatty acids with different types of liposomes and their use in the rearing of gilthead sea bream (Sparus aurata) larvae - Aquaculture 251: 491-508.

Monroig O., Navarro J.C., Amat F., Gonzales P., Hontoria F. 2007 - Oxidative stability and changes in the particle size of liposomes used in the Artemia enrichment - Aquaculture 266: 200-210.

Najdegerami E.H., Baruah K., Shiri A., Rekecki A., Van den Broeck W., Sorgeloos P., Boon N., Bossier P., De Schryver P. 2015 - Siberian sturgeon (Acipenser baerii) larvae fed Artemia nauplii enriched with poly-b-hydroxybutyrate (PHB): effect on growth performance, body composition, digestive enzymes, gut microbial community, gut histology and stress tests - Aquac. Res. 46: 801-812.

Navarro J.C., Henderson R.J., McEvoy L.A., Bell M.V., Amat F. 1999 - Lipid conversions during enrichment of Artemia - Aquaculture 174: 155-166.

Navarro J.C., Sargent J.R. 1992 - Behavioural differences in starving Clupea harengus L. larvae correlate with body levels of essential fatty acids - J. Fish Biol. 41: 509-513.

Nelis H.J.C.F., Lavens P., Van Steenberge M.M.Z., Sorgeloos P., Criel G.R., De Leenheer A.P. 1988 - Qualitative and quantitative changes in the carotenoids during development of the brine shrimp Artemia - J. Lipid Res. 29: 491-499.

Nguyen V.T., Satoh S., Haga Y., Fushimi H., Kotani T. 2008 - Effect of zinc and manganese supplementation in Artemia on growth and vertebral deformity in red sea bream (Pagrus major) larvae - Aquaculture 285: 184-192.

Nhu V.C., Dierckens K., Nguyen T.H., Tran M.T., Sorgeloos P. 2009 - Can umbrella-stage Artemia franciscana substitute enriched rotifers for Cobia (Rachycentron canadum) fish larvae? - Aquaculture 289: 64-69.

Postel L., Fock H., Hagen W. 2000 - Biomass and abundance - In: Zooplankton Methodology Manual (Eds) R. Harris, P. Wiebe, J. Lenz, H.-R. Skjoldal, M. Huntley, ICES, London, Academic Press: 83-192.

Prusińska M., Chepurkina M., Wiszniewski G., Duda A., Kolman R. 2011 - Preliminary results of rearing larval Russian sturgeon (Acipenser gueldenstaedtii) fed enriched live feed - In: New aquaculture species. Breeding, rearing, prophylactics (Eds) Zakêoe Z., Demska-Zakêoe K., Kowalska A., Wyd. IRS, Olsztyn: 45-52 (in Polish).

Prusińska M., Kushniryk O., Khudyi O., Khuda L., Kolman R. 2015 - Impact of enriching larval brine shrimp (Artemia sp.) with a supplement containing polyunsaturated fatty acids on their growth and mortality - Arch. Pol. Fish. 23: 149-154.

Sargent J.R., Bell J.G., Henderson R.J., Tocher D.R. 1995 - Requirement criteria for essential fatty acids - J. Appl. Ichthyol. 11: 183-198.

Skljarov O.J., Fartushok N.V., Solskyj J., Velykij M.M., Bondarchuk T.I., Duma D. 2008 - Enzyme biochemistry. Enzyme diagnostics. Enzyme pathology. Enzyme therapy - Skljarov, Vidavnitstvo “Medicina”, Kyiv, Ukraine, 450 p. (in Ukrainian).

Tocher D. 2003 - Metabolism and functions of lipids and fatty acids in teleost fish - Rev. Fish. Sci. 11: 107-184.

Vite-García N., Simoes N., Arjona O., Mascaro M., Palacios E. 2014 - Growth and survival of Hippocampus erectus (Perry, 1810) juveniles fed on Artemia with different HUFA levels - Lat. Am. J. Aquat. Res. 42: 150-159.

Fisheries & Aquatic Life

The Journal of Stanislaw Sakowicz Inland Fisheries Institute

Journal Information

CiteScore 2017: 0.53

SCImago Journal Rank (SJR) 2017: 0.204
Source Normalized Impact per Paper (SNIP) 2017: 0.493


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 378 378 24
PDF Downloads 82 82 18