Condition of sows during reproductive activity depending on lipid metabolism gene (DGAT1) polymorphism

Magdalena Szyndler-Nędza 1 , Katarzyna Piórkowska 2  and Katarzyna Ropka-Molik 2
  • 1 Department of Animal Genetics and Breeding, National Research Institute of Animal Production, 32-083 Balice n. , Kraków, Poland
  • 2 Laboratory of Genomics, National Research Institute of Animal Production, 32-083 Balice n. , Kraków, Poland

Abstract

Mutations at gene loci associated with body lipid metabolism may affect not only carcass and meat quality traits in young animals but also the production results of mother sows. The objective of the study was to determine the effect of mutations in the DGAT1 gene, found in Polish populations of maternal breeds, on condition of gilts and later on condition and reproductive performance of sows during three reproductive cycles. The study involved 118 gilts of maternal breeds (Polish Large White (PLW ) and Polish Landrace (PL)). Gilts and subsequently sows were monitored for body weight (BW) and P2 backfat thickness over three reproductive cycles and their litters were analysed for the number and body weight of the piglets. A total of 354 litters of pigs were evaluated. In the analysed pig populations, a single mutation (A/G) in the DGAT1 gene (rs45434075) had a significant effect on deposition of subcutaneous fat only in the PLW gilts (P2G, AA,AG<GG P≤0.05 and P2F r=214 P≤0.01). As regards the G/A 3’UTR polymorphism of the DGAT1 gene (rs342152658), this mutation is negatively correlated only in the PL sows with fatness during their reproductive life and with BW of the piglets reared to 21 d of age. Sows of the DGAT1GG 3’UTR genotype had significantly lower P2F backfat thickness (P≤0.05) and, during a 21-d lactation, reared lighter piglets (P≤0.01) compared to sows of the same breed with the DGAT1AG 3’UTR genotype. The analysed traits were also affected by the interaction between mutations in the DGAT1 gene of the sows of maternal breeds (DGAT1/DGAT1 3’UTR). Sows of the DGAT1GG/GG genotype were characterised by the highest backfat thickness on selection day, and during three reproductive cycles they reared significantly more piglets compared to sows of the DGAT1AA/AG genotype. Heterozygous sows (AG/AG) reared significantly heavier piglets than sows of the AA/GG and AG/GG genotypes (P≤0.05).

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Beyga K., Rekiel A. (2009). Effect of body condition and oxitocin administration on the course of parturition in sows and results of piglet rearing (in Polish). Rocz. Nauk. Zoot., 36: 45-53.

  • Buhman K.K., Smith S.J., Stone S.J., Repa J.J., Wong J.S., Knapp F.F.J., Burri B.J., Hamilton R.L., Abumrad N.A., Farese R.V.J. (2002). DGAT1 is not essential for intestinal triacylglycerol absorption or chylomicron synthesis. J. Biol. Chem., 277: 25474-25479.

  • Decaluwé R., Maes D., Declerck I., Cools A., Wuyts B., De Smet S., Janssens G.P.J. (2013). Changes in back fat thickness during late gestation predict colostrum yield in sows. Animal, 7: 1999-2007.

  • Fan B., Onteru S.K., Plastow G.S., Rothschild M.F. (2009). Detailed characterization of the porcine MC4Rgene in relation to fatness and growth. Anim. Genet., 40: 401-409.

  • Hausman G.J., Dodson M.V., Ajuwon K., Hausman G.J., Dodson M.V., Ajuwon K., Azain M., Barnes K.M., Guan L.L., Jiang Z., Poulos S.P., Sainz R.D., Smith S., Spurlock M., Novakofski J., Fernyhough M.E., Bergen W.G. (2009). Board sponsored invited review: the biology and regulation of preadipocytes and adipocytes in meat animals. J. Anim. Sci., 87: 1218-1246.

  • Kim K.S., Lee J.J., Shin H.Y., Choi B.H., Lee C.K., Kim J.J., Cho B. W., Kim T.H. (2006). Association of melanocortin 4 receptor (MC4R) and high mobility group AT-hook 1 (HMGA1) polymorphisms with pig growth and fat deposition traits. Anim. Genet., 37: 419-421.

  • Knecht D., Środoń S., Duziński K. (2014). In vivo evaluation of the fat content and muscularity f gilts with different genotypes using an Aloka SSD-500 ultrasound scanner in relation to selected reproductive performance indicators (in Polish). Rocz. Nauk. PTZ., 10: 25-35.

  • Lopaschuk G.D., Ussher J.R., Folmes C.D., Jaswal J.S., Stanley W.C. (2010). Myocardial fatty acid metabolism in health and disease. Physiol. Rev., 90: 207-258.

  • López- Buesa P., Burgos C., Galve A., Varona L. (2014). Joint analysis of additive, dominant and first-order epistatic effects of four genes (IGF2, MC4R, PRKAG3 and LEPR) with known effects on fat content and fat distribution in pigs. Anim. Genet., 45: 133-137.

  • Lv Y., Guan W., Qiao H., Wang C., Chen F., Zhang Y., Liao Z. (2015). Veterinary medicine and omics (veterinomics): Metabolic transition of milk triacylglycerol synthesis in sows from late pregnancy to lactation. OMICS, 19: 602-616.

  • Matysiak B., Kawęcka M., Kołodziej A., Sosnowska A. (2007). Relationships between body weight of primiparous sows in pregnancy and their reproductive performance. Acta Sci. Pol. Zootechnica, 6: 25-32.

  • Migdał W., Koczanowski J., Klocek C., Tuz R., Paściak P., Wojtysiak D., Orlicki S. (2005). Chemical composition of colostrum and milk from Polish Landrace and Polish Large White sows. Ann. Anim. Sci., Suppl., 1: 43-46.

  • Nonneman D., Rohrer G.A. (2002). Linkage mapping of porcine DGAT1 toaregion of chromosome 4 that contains QTLfor growth and fatness. Anim. Genet., 33: 472-473.

  • Rekiel A., Więcek J., Beyga K. (2011). Analysis of the relationship between fatness of late pregnant and lactating sows and selected lipid parameters of blood, colostrum and milk. Ann. Anim. Sci., 11: 487-495.

  • Rekiel A., Więcek J., Kuczyńska B., Bartosik J., Warda A., Furman K. (2015). Effect of the backfat thickness at point P2 during insemination on the selected parameters of colostrum and milk of the sows. Ann. Warsaw Univ. Live Sci. - SGGW Anim. Sci., 54: 153-160.

  • Renaville B., Bacciu N., Lanzoni M., Corazzin M., Piasentier E. (2015). Polymorphism of fat metabolism genes as candidate markers for meat quality and production traits in heavy pigs. Meat Sci., 17: 220-223.

  • Szczerbal I., Li L., Stachowiak M., Chmurzyńska A., Maćkowski M., Winter A., Flisikowski K., Fries R., Świtoński M. (2007). Cytogenetic mapping of DGAT1, PPARA, ADIPOR1 and CREBgenes in the pig. J. Appl. Genet., 48: 73-76.

  • Szyndler - Nędza M., Piórkowska K. (2015). Effect of DGAT1 gene mutation in sows of damline on the composition of the produced milk and piglet rearing during 21-day lactation. African J. Biotech., 14: 2478-2483.

  • Szyndler - Nędza M., Tyra M., Ropka- Molik K., Piórkowska K., Mucha A., Różycki M., Koska M., Szulc K. (2013). Association between LEPRand MC4Rgenes polymorphism and composition of milk from sows of dam line. Mol. Biol. Rep., 40: 4339-4347.

  • Terman A., Kumalska M. (2012). The effect ofa SNPin ESRgene on the reproductive performance traits in Polish sows. Russian J. Genet., 48: 1260-1263.

  • Weisz F., Urban T., Chalupowa P., Knoll A. (2011). Association analysis of seven candidate genes with performance traits in Czech Large White pigs. Czech J. Anim. Sci., 56: 337-344.

  • Wientjes J.G.M., Soede N.M., Knol E.F., Vanden Brand H., Kemp B. (2013). Piglet birth weight and litter uniformity: Effects of weaning-to-pregnancy interval and body condition changes in sows of different parities and crossbred lines. J. Anim Sci., 91: 2099-2107.

  • Young M.G., Tokach M.D., Aherne F.X., Main R.G., Dritz S.S., Goodband R.D., Nelssen J.L. (2004). Comparison of three methods of feeding sows in gestation and the subsequent effects on lactation performance. J. Anim. Sci., 82: 3058-3070.

  • Zhang C.Y., Wang Z., Bruce H.L., Janz J., Goddard E., Moore S., Plastow G.S. (2014). Associations between single nucleotide polymorphisms in 33 candidate genes and meat quality traits in commercial pigs. Anim. Genet., 45: 508-516.

  • Zuo Zuo Y. (2008). Studies on SNPs of DGAT1 gene and its association with partial economic traits in pigs. Dissertation, Hunan Agricultural University. http://www.dissertationtopic.net/doc/497147.

OPEN ACCESS

Journal + Issues

Search