Prevalence and some virulence genes of Escherichia coli O157 isolated from chicken meats and giblets

Open access

Abstract

Escherichia coli O157 related foodborne illnesses continue to be one of the most important global public health problems in the world. This study aims to determine E. coli O157 prevalence in 375 chicken meat parts and giblets. The samples were collected randomly from several supermarkets and butchers in Diyarbakir, a city in southeast Turkey. They were analyzed and confirmed using the immunomagnetic separation (IMS), Vitek® 2 microbial identification system and polymerase chain reaction (PCR) method. This study also aims to detect the presence of fliCH7, eaeA, stx1, stx2 and hlyA genes by using PCR. The overall E. coli O157 prevalence in chicken meat parts and giblets was 1.3%. All of the E. coli O157 isolates carried rfbEO157 and eaeA genes; but not any fliCH7 and hlyA genes. The E. coli O157 isolates obtained from drumstick and breast meat carried either stx1 or stx2 genes, which were related to important virulence factors of the disease.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Abong’o B.O. Momba M.N.B. (2008). Prevalence and potential link between E. coli O157: H7 isolated from drinking water meat and vegetables and stools of diarrheic confirmed and non-confirmed HIV/AIDSpatients in the Amathole District-South Africa. J. Appl. Microbiol. 105: 424-431.

  • Adams N.L. Byrne L. Smith G.A. Elson R. Harris J.P. Salmon R. Smith R. O’Brien S.J. Adak G.K. Jenkins C. (2016). Shiga toxin-producing Escherichia coli O157 England and Wales 1983-2012. Emerg. Infect. Dis. 22 p. 590.

  • Ahmed A.M. Shimamoto T. (2014). Isolation and molecular characterization of Salmonella enterica Escherichia coli O157: H7 and Shigella spp. from meat and dairy products in Egypt. Int. J. Food Microbiol. 168: 57-62.

  • Akbar A. Sitara U. Ali I. Iftikhar Khan M. Phadungchob T. Anal A.K. (2014). Presence of Escherichia coli in poultry meat: Apotential food safety threat. Int. Food Res. J. 21: 941-945.

  • Allerberger F. (2015). Poultry and human infections. Clin. Microbiol. Infect. 22: 101-102.

  • Alonso M.Z. Lucchesi P.M.A. Rodríguez E.M. Parma A.E. Padola N.L. (2012). Enteropathogenic (EPEC) and Shigatoxigenic Escherichia coli (STEC) in broiler chickens and derived products at different retail stores. Food Control 23: 351-355.

  • Baker C.A. Rubinelli P.M. Park S.H. Carbonero F. Ricke S.C. (2016). Shiga toxinproducing Escherichia coli in food: Incidence ecology and detection strategies. Food Control 59: 407-419.

  • Cagney C. Crowley H. Duffy G. Sheridan J.J. O’Brien S. Carney E. Anderson W. Mcdowell D.A. Blair I.S. Bishop R.H. (2004). Prevalence and numbers of Escherichia coli O157: H7 in minced beef and beef burgers from butcher shops and supermarkets in the Republic of Ireland. Food. Microbiol. 21: 203-212.

  • Chinen I. Epszteyn S. Melamed C.L. Aguerre L. Espinosa E.M. Motter M.M. Baschkier A. Manfredi E. Miliwebsky E. Rivas M. (2009). Shiga toxin-producing Escherichia coli O157 in beef and chicken burgers and chicken carcasses in Buenos Aires Argentina. Int. J. Food. Microbiol. 132: 167-171.

  • Erdogan H. Levent B. Erdogan A. Gulesen R.. Arslan H. (2011). Investigation of verotoxigenic Escherichia coli O157:H7 incidence in gastroenteritis patients. Mikrobiyol. Bul. 45: 519-525.

  • Eurosurveillance (1999). Sporadic cases of VTEC O157 infection associated with travel to southern Turkey 3: 443-446. Available online: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=1288

  • Fields P.I. Blom K. Hughes H.J. Helsel L.O. Feng P. Swaminathan B. (1997). Molecular characterization of the gene encoding Hantigen in Escherichia coli and development ofa PCR-restriction fragment length polymorphism test for identification of E. coli O157: H7 and O157: NM. J. Clin. Microbiol. 35: 1066-1070.

  • Fratamico P.M. Bagi L.K. Pepe T. (2000). Amultiplex polymerase chain reaction assay for rapid detection and identification of Escherichia coli O157: H7 in foods and bovine feces. J. Food. Prot. 63: 1032-1037.

  • Fukushima H. Seki R. (2004). High numbers of Shiga toxin-producing Escherichia coli found in bovine faeces collected at slaughter in Japan. FEMS Microbiol. Lett. 238: 189-197.

  • Gulesen R. Levent B. Demir T. Akgeyik M. Kuran S. (2016). Characterization of the Shiga toxin-producing Escherichia coli isolated from human between 2011 and 2014. Jpn. J. Infect. Dis. DOI: 10.7883/yoken.JJID.2015.346.

  • Hascelik G. Akan O.A. Diker S. Baykal M. (1991). Campylobacter and enterohaemorrhagic Escherichia coli (EHEC) associated gastroenteritis in Turkish children. J. Diarrhoeal Dis. Res. 9: 315-317.

  • Heiman K.E. Mody R.K. Johnson S.D. Griffin P.M. Gould L.H. (2015). Escherichia coli O157 outbreaks in the United States 2003-2012. Emerg. Infect. Dis. 21 p. 1293.

  • Kalin R. Ongor H. Cetinkaya B. (2012). Isolation and molecular characterization of Escherichia coli O157 from broiler and human samples. Foodborne Pathog. Dis. 9: 313-318.

  • Karadal F. Ertas N. Hizlisoy H. Abay S. Al S. (2013). Prevalence of Escherichia coli O157: H7 and their verotoxins and Salmonella spp. in processed poultry products. J. Food Saf. 33: 313-318.

  • Karmali M.A. Gannon V. Sargeant J.M. (2010). Verocytotoxin-producing Escherichia coli (VTEC). Vet. Microbiol. 140: 360-370.

  • Kim S.R. Yoon Y. Seo M.K. Kim W.I. Shim W.B. Chung D. Yun J.C. Ryu K.Y. Kim B.S. (2014). Modification of methods for detection of Escherichia coli O157: H7 on produce. Food Sci. Biotechnol. 23: 1349-1356.

  • Manges A.R. (2015). Escherichia coli and urinary tract infections: the role of poultry-meat. Clin. Microbiol. Infect. 22: 122-129.

  • Maurer J.J. Schmidt D. Petrosko P. Sanchez S. Bolton L. Lee M.D. (1999). Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR. Appl. Environ. Microbiol. 65: 2954-2960.

  • Messens W. Bolton D. Frankel E. Liebana E. McLauchlin J. Morabito S. Oswald E. Threlfall E.J. (2015). Defining pathogenic verocytotoxin-producing Escherichia coli (VTEC) from cases of human infection in the European Union 2007-2010. Epidemiol. Infect. 143: 1652-1661.

  • Mir R.A. Weppelmann T.A. Elzo M. Ahn S. Driver J.D. Jeong K.C. (2016). Colonization of beef cattle by Shiga toxin-producing Escherichia coli during the first year of life:acohort study. Plo Sone 11: e0148518. doi:

    • Crossref
    • Export Citation
  • Momtaz H. Jamshidi A. (2013): Shiga toxin-producing Escherichia coli isolated from chicken meat in Iran: Serogroups virulence factors and antimicrobial resistance properties. Poultry Sci. 92: 1305-1313.

  • Nataro J.P. Kaper J.B. (1998). Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201.

  • Ongor H. Kalin R. Cetinkaya B. (2007). Investigations of Escherichia coli O157 and some virulence genes in samples of meat and faeces from clinically healthy cattle in Turkey. Vet. Rec. 161: 392-394.

  • Paton A.W. Paton J.C. (1998). Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCRassays for stx 1 stx 2 eae A enterohemorrhagic E. coli hly A rfb O111 and rfb O157. J. Clin. Microbiol. 36: 598-602.

  • Pilipcinec E. Tkáciková L. Naas H.T. Cabadaj R. Mikula I. (1999). Isolation of verotoxigenic Escherichia coli O157 from poultry. Folia Microbiol. 44: 455-456.

  • Sami M. Roya F. (2007). Prevalence of Escherichia coli O157: H7 on dairy farms in Shiraz Iran by immunomagnetic separation and multiplex PCR. Iran J. Vet. Res. 4: 319-324.

  • Schmidt H. Beutin L. Karch H. (1995). Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157: H7 strain EDL 933. Infect. Immun. 63: 1055-1061.

  • Schouten J.M. Van de Giessen A.W. Frankena K. De Jong M.C.M. Graat E.A.M. (2005). Escherichia coli O157 prevalence in Dutch poultry pig finishing and veal herds and risk factors in Dutch veal herds. Prev. Vet. Med. 70: 1-15.

  • Smith - Palmer A. Locking M. Reilly B. Fisher I.S. (2005). Cluster of E. coli O157 infections in Scottish tourists returning from southwest Turkey July-August (2005). Euro. Surveill. 10: E050818.2.

  • Tarr P.I. Gordon C.A. Chandler W.L. (2005). Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome. The Lancet 365: 1073-1086.

  • Wani S.A. Samanta I. Bhat M.A. Nishikawa Y. (2004). Investigation of shiga toxin-producing Escherichia coli in avian species in India. Lett. Appl. Microbiol. 39: 389-394.

Search
Journal information
Impact Factor


IMPACT FACTOR 2018: 1.515
5-year IMPACT FACTOR: 1.246

CiteScore 2018: 1.4

SCImago Journal Rank (SJR) 2018: 0.509
Source Normalized Impact per Paper (SNIP) 2018: 0.869

Cited By
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 454 204 13
PDF Downloads 246 150 7