Physiological Threshold of Somatic Cell Count in Milk of Polish Heath Sheep and Polish Lowland Sheep

Wiesław Piotr Świderek 1 , Krystyna Małgorzata Charon 1 , Anna Winnicka 2 , Joanna Gruszczyńska 1  and Mariusz Pierzchała 3
  • 1 Department of Animal Genetics and Breeding, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland
  • 2 Department of Pathology and Laboratory Diagnostics, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland
  • 3 Department of Molecular Cytogenetics, Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzębiec, Postępu 36A, 05-552 Magdalenka, Poland

Abstract

The study was conducted on 320 sheep of two breeds: Polish Heath Sheep (PHS) and Polish Lowland Sheep (PLS). Udder health status was assessed based on somatic cell count (SCC) and bacteriological examination of milk. Cells of the immune system (leukocyte populations and lymphocyte subpopulations) were identified in the blood and milk of sheep by flow cytometry, using a panel of specific monoclonal antibodies and fluorescent dyes. The study showed that the highest proportion of neutrophils and a lower percentage of lymphocytes (CD4+, CD8+, CD19+, WC1-N2+) in milk and blood occurred in sheep of both breeds in which milk SCC ranged between 201 and 300×103 cells/ml. In light of existing research, these results suggest that fluctuations in somatic cell count of ewe milk are physiologically determined up to 200×103cells/ml and result from udder health disturbances above this level.

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  • Albenzio M., Santillo A., Caroprese M., Ruggieri D., Ciliberti M., Sevi A. (2012). Immune competence of the mammary gland as affected by somatic cell and pathogenic bacteria in ewes with subclinical mastitis. J. Dairy Sci., 95: 3877-3887.

  • Asai K.I., Komine Y., Kozutsumi T., Yamaguchi T., Komine K.I., Kumagai K. (2000). Predominant subpopulations of Tlymphocytes in the mammary gland secretions during lactation and intraepithelial Tlymphocytes in the intestine of dairy cows. Vet. Immunol. Immunopathol., 73: 233-240.

  • Banos G., Wall E., Coffey M.P., Bagnall A., Gillespie S., Russell G.C., Mc Neil - ly T.N. (2013). Identification of immune traits correlated with dairy cow health, reproduction and productivity. PLo S ONE 8(6): e65766. doi:10.1371/journal.pone.0065766.

  • Benić M., Habrun B., Kompes G., MihaljevićŽ., CvetnićŽ., Cergolj M., Maće- šić N. (2012). Cell content in milk from cows with S. aureus intramammary infection. Vet. Arhiv., 82: 411-422.

  • Bergonier D., Berthelot X. (2003). New advances in epizootiology and control of ewe mastitis. Livest. Prod. Sci., 79: 1-16.

  • Bonelli P., Dimauro C., Re R., Pilo G., Dore S., Cannas A.E., Nicolussi P.S. (2013). Peripheral blood and milk leukocytes subsets of lactating Sarda ewes. Ital. J. Anim. Sci., 12: 208-212.

  • Boulanger J.D., Bureau F., M.E., Lotte D., Mainil J., Lekeux P. (2003). Increased nuclear factorκBactivity in milk cells of mastitis-affected cows. J. Dairy Sci., 86: 1259-1267.

  • Burton J.L., Kehrli J.R. (1996). Effects of dexamethasone on bovine circulating Tlymphocyte populations. J. Leukoc. Biol., 59: 90-99.

  • Chaffer M., Leitner G., Winkler M., Glickman A., Krifucks O., Ezra E., Sa - ran A. (1999). Coagulase-negative staphylococci and mammary gland infections in cows. Zentralbl. Vetmed. B, 46: 707-712.

  • Concha C., Saad A., Holmberg O. (1995). Comparison of mammary gland T-cell proliferative responses and proportions of three T-lymphocyte populations (CD, CD4 and CD8) between late lactation and mid-involution. IDF International Mastitis Seminar, Tel-Aviv Israel, 28 May - 1 June, 1995, pp. 91-94.

  • De La Cruz M., Serrano E., Montoro V., Marco J., Romeo M., Baselga R., Albi - zu L., Amorena B. (1994). Etiology and prevalence of subclinical mastitis in the Manchega sheep at mid-lactation. Small Rumin. Res., 14: 175-180.

  • Fawcett T. (2006). An introduction to ROCanalysis. Pattern Recogn. Lett., 27: 861-874.

  • Filev F. (1972). Microbiologicni proucvania na ovceto mljako. VII. Elektronen metod zaopredeljane broja kletkie. Vet. Med. Nauk., 9: 33-36.

  • Fruganti G., Ranucci S., Tesei B., Valente C. (1985). Valutazione dello stato sanitario della mammella di pecore durante un intero ciclo di lattazione. Clin. Vet., 108: 286-296.

  • Fthenakis G.C., El-Masannt E.T.S., Booth J.M., Jones J.E.T. (1991). Somatic cell counts of ewes’milk. Br. Vet. J., 147: 575-581.

  • Gonzalez-Rodriguez M.C., Gonzalo C., San Primitivo F., Carmenes P. (1995). Relationship between somatic cell count and intramammary infection of the half udder in dairy ewes. J. Dairy Sci., 78: 2753-2759.

  • Gonzalo C., Carriedo J.A., Baro J.A., Primitivo F. (1994). Factors influencing variation of test day milk-yield, somatic cell count, fat and protein in dairy sheep. J. Dairy Sci., 77: 1537-1542.

  • Green T.J. (1984). Use of somatic cell counts for detection of subclinical mastitis in ewes. Vet. Res., 114, p. 43.

  • Hurley D.J., Kensinger M.H., Mastro A.M., Wilson R.A. (1990). An evaluation of the mononuclear cells derived from bovine mammary gland dry secretions using leukocyte antigen specific monoclonal antibodies, light scattering properties and non-specific esterase staining. Vet. Immunol. Immunopathol., 25: 177-193.

  • Jones J.E.T. (1991). Mastitis in Sheep. Breeding for Disease Resistance in Farm Animals. Owen J.B., Axford R.F.E. (eds). CAB Int., Wallingford, UK, pp. 412-423.

  • Lafi S.Q., Al Majali A.M., Rousan M.D., Alaweneh J.M. (1998). Epidemiological studies of clinical and subclinical ovine mastitis in Awassi sheep in northern Jordan. Prev. Vet. Med., 33: 171-181.

  • Lascelles J. (1979). Heme-deficient mutants of Staphylococcus aureus. Meth. Enzymol., 56: 172-178.

  • Le Roux Y., Laurent F., Moussaoui F. (2003). Polymorphonuclear proteolytic activity and milk composition change. Vet. Res., 34: 1-17.

  • Lee J.W., O’ Brien C.N., Guidry A.J., Paape M.J., Shafer-Weaver K.A., Zhao X. (2005). Effect ofatrivalent vaccine against Staphylococcus aureus mastitis lymphocyte subpopulations, antibody production, and neutrophil phagocytosis. Can. J. Vet. Res., 69: 11-18.

  • Leitner G., Shoshani E., Krifucks O., Chaffer M., Saran A. (2000). Milk leukocyte population patterns in bovine udder infection of different etiology B. Infect. Dis. Public. Heath., 47: 581-589.

  • Leitner G., Chaffer M., Zamir S., Mor T., Glickman A., Winkler M., Weisblit L., Saran A. (2001). Udder disease etiology, milk somatic cell count and NAGase activity in Israeli Assaf sheep throughout lactation. Small Rumin. Res., 39: 107-112.

  • Leitner G., Eligulashvily R., Krifucks O., Perl S., Saran A. (2003). Immune cell differentiation in mammary gland tissues and milk of cows chronically infected with Staphylococcus aureus. B Infect. Dis. Vet. Pub. Health, 50: 45-52.

  • Mavrogenis A.P., Koumas A., Kakoyiannis C.K., Taliotis C.H. (1995). Use of somatic cell count for the detection of subclinical mastitis in sheep. Small Rumin. Res., 17: 79-84.

  • Mørk T., Waage S., Tollersrud T., Kvitle B., Sviland S. (2007). Clinical mastitis in ewes; bacteriology, epidemiology and clinical features. Acta Vet. Scand., 49, p. 23, doi:10.1186/1751-0147-49-23.

  • Mukasa A., Yoshida H., Kobayashi N., Matsuzaki G., Nomoto K. (1998). Gamma delta Tcells in infection-induced and autoimmune-induced testicular inflammation. Immunol., 95: 395-401.

  • Ozenc E., Seker E., Baki Acar D., Birdane M.K., Darbaz I., Dogan N. (2011). The importance of staphylococci and threshold value of somatic cell count for diagnosis of sub-clinical mastitis in Pirlak sheep at mid-lactation. Reprod. Domest., Anim., 46: 970-974.

  • Park Y.H., Jung S.C., Moon J.S. (1994). Asubset of mammary glandγδTlymphocytes down regulates Bo CD4 Tlymphocyte response to Staphyloccocus aureus in cattle with intramammary infection. Korean J. Immunol., 16: 19-27.

  • Park Y.H., Joo Y.S., Park J.H., Moon J.S., Kim S.H., Kwon N.H., Ahn J.S., Davis W.C., Davies C.J. (2004). Characterization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitis-resistant and susceptible cows. J. Vet. Sci., 5: 29-39.

  • Pengov A. (2001). The role of coagulase-negative Staphylococcus spp. and associated somatic cell counts in the ovine mammary gland. J. Dairy Sci., 84: 572-574.

  • Persson- Waller K., Colditz I.G. (1998). Expression of surface antigens on blood and mammary leukocytes in lactating and dry ewes. Vet. Immunol. Immunopathol., 62: 273-278.

  • Persson- Waller K., Colditz I.G. (1999 a). The effect of experimental infectious mastitis on leukocyte subpopulations and cytokine production in non-lactating ewes. J. Vet. Med. B, 46: 289-299.

  • Persson- Waller K., Colditz I.G. (1999 b). Effect of intramammary infusion ofβ-1,3-glucan or interleukin-2 on leukocyte subpopulations in mammary glands of sheep. Am. J. Vet. Res., 60: 703-707.

  • Piccinini R., Binda E., Belotti M., Casirani G., Zecconi A. (2005). Comparison of blood and milk non-specific immune parameters in heifers after calving in relation to udder health. J. Vet. Res., 36: 747-757.

  • Pillai S.R., Kunze E., Sordillo L.M., Jayarao B.M. (2001). Application of differential inflammatory cell count asatool to monitor udder health. J. Dairy Sci., 84: 1413-1420.

  • Riggio V., Portolano B., Bovenhuis H., Bishop S.C. (2010). Genetic parameters for somatic cell score according to udder infection status in Valle del Belice dairy sheep and impact of imperfect diagnosis of infection. Genet. Sel. Evol., doi: 10.1186/1297-9686-42-30.

  • Riggio V., Pesce L.L., Morreale S., Portolano B. (2013). Receiver-operating characteristic curves for somatic cell scores and California mastitis test in Valle del Belice dairy sheep. Vet. J., 196: 528-532.

  • Riollet C., Rainard P., Poutrel B. (2000). Cells and cytokines in inflammatory secretions of bovine mammary gland. Adv. Exp. Med. Biol., 480: 247-258.

  • Rivas A.L., Quimby F.W., Coksaygan O., Olmstead L., Lein D.H. (2000). Longitudinal evaluation of CD4+ and CD8+ peripheral blood and mammary gland lymphocytes in cows experimentally inoculated with Staphylococcus aureus. Can. J. Vet. Res., 64: 232-237.

  • Romeo M., Ziluga I., Marco J. (1998). Diagnostico in situ de la infeccion mamaria mediante palpacion, California mastitis test su seguimiento mediante recuento de celulas somaticas. Ovis, 59: 61-77.

  • Saunders B.M., Frank A.A., Cooper A.M., Orme I.M. (1998). Role of gamma delta Tcells in immunopathology of pulmonary Mycobacterium avium infection in mice. Infect. Immunol., 66: 5508-5514.

  • Schwarz D., Diesterbeck U.S., König S., Brügemann K., Schlez K., Zschöck M., Wolter W., Czerny C.P. (2011). Flow cytometric differential cell counts in milk for the evaluation of inflammatory reactions in clinically healthy and subclinically infected bovine mammary glands. J. Dairy Sci., 94: 5033-5044.

  • Soltys J., Quinn M.T. (1999). Selective recruitment of T-cell subsets to the udder during Staphylococcal and Streptococcal mastitis: Analysis of lymphocyte subsets and adhesion molecule expression. Infection and Immunity, 67: 6293-6302.

  • Sordillo L.M., Nickerson S.C., Akers R.M., Oliver S.P. (1987). Secretion composition during bovine mammary involution and the relationship with mastitis. Inter. J. Bioch., 19: 1165-1172.

  • Sordillo L.M., Nickerson S.C., Akers R.M. (1989). Pathology of Staphylococcus aureus mastitis during lactogenesis: relationships with bovine mammary structure and function. J. Dairy Sci., 72: 228-240.

  • Stefanakis A., Boscs C., Alaxopoulos C., Samartzi F. (1995). Frequency of subclinical mastitis and observation on somatic cell counts in ewe’s milk in northern Greece. Anim. Sci., 61: 69-76.

  • Suarez V.H., Busetti M.R., Miranda A.O., Calvinho L.F., Bedotti D.O., Canave - sio V.R. (2002). Effect of infectious status and parity on somatic cell count and California mastitis test in Pampinta dairy ewes. J. Vet. Med. B Infect. Dis. Vet. Public. Health., 49: 230-234.

  • Taylor B.C., Dellinger J.D., Cullor J.S., Stott J.L. (1994). Bovine milk lymphocytes display the phenotype of memory Tcells and predominantly CD8+. Cell. Immunol., 156: 245-253.

  • Tolone M., Riggio V., Portolano B. (2013). Estimation of genetic and phenotypic parameters for bacteriological status of the udder, somatic cell score, and milk yield in dairy sheep using a threshold animal model. Livest. Sci., 151: 134-139.

  • Travniček M., Petrik P., Balašcák J., Federic F. (1976). Vhodnost pouzitia niektorych metodikvprevencii mastitid bahnicvpodmienkach strojového dojenia. Veterinarstvi, 26: 508-509.

  • Watkins G.H., Jones J.E.T. (2004). Observations on mastitis in lowland sheep at and after weaning. Proc. Sheep Vet. Soc., 27: 61-64.

  • Watkins G.H., Burriel A.R., Jones J.E.T. (1991). Afield investigation of subclinical mastitis in sheep in southern England. Br. Vet. Rec., 147: 413-431.

  • Winnicka A., Kluciński W., Hoser G., Sikora J., Kawiak J. (1999). Flow cytometry analysis of milk and peripheral blood cells from goats during lactation. J. Vet. Med., 46: 459-464.

  • Winnicka A., Kluciński W., Kawiak J., Hoser G., Sikora J. (2000). Effect of Baypamun on blood leucocytes in normal and dexamethasone treated goats. J. Vet. Med., 47: 385-394.

  • Winter P., Colditz I.G. (2002). Immunological responses of the lactating ovine udder following experimental challenge with Staphylococcus epidermidis. Vet. Immunol. Immunopathol., 89: 57-65.

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