Antiproliferative Properties Against Human Breast, Cervical and Ovarian Cancer Cell Lines, and Antioxidant Capacity of Leaf Aqueous Ethanolic Extract from Cotinus coggygria Scop.

Z. Gospodinova 1 , N. Bózsity 2 , M. Nikolova 3 , M. Krasteva 1  and I. Zupkó 2
  • 1 Laboratory of Genome Dynamics and Stability, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria
  • 2 Department of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, Hungary
  • 3 Department of Applied Botany, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria


Cotinus coggygria Scop. leaf aqueous ethanolic extract was examined for its in vitro antiproliferative and antioxidant activity. Antiproliferative effect was assessed on four human gynecological cancer cell lines: breast (MCF7, T47D), cervical (HeLa) and ovarian (A2780) and compared to the cell growth inhibitory effect on non-cancerous breast epithelial cell line MCF10A using MTT cell proliferation assay. Radical scavenging assay with DPPH was applied to evaluate antioxidant potential of the extract. The obtained results showed that the herb inhibited cell growth of all of the tested cancer cell lines and the highest was the cytostatic effect on A2780 cells with a half maximal inhibitory concentration (IC50) value of 30.8 μg/ml. For the other cell lines the IC50 values were in the range of 55-122.7 μg/ml. Additionally, the extract exerted considerably weaker reduction in cell proliferation of the non-cancerous cell line MCF10A compared to cancer cells, which indicates for antiproliferative selectivity. C. coggygria extract showed high free radical scavenging activity with an IC50 value of 11.2 μg/ml. The obtained data provide evidence for pharmacological potential of the tested extract and future more detailed studies concerning the molecular mechanisms of the anticancer effect of the herb are needed.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Ajaib M, Khan ZUD, Khan N, Wahab M. Ethnobotanical studies on useful shrubs of District Kotli, Azad Jammu & Kashmir, Pakistan. Pak J Bot, 2010;42(3):1407-1415.

  • 2. Antal DS, Ardelean F, Pinzaru I et al. Effects of cyclodextrin complexation on the anti-cancer effects of Cotinus coggygria extract and its constituents, butein and sulfuretin. Revista de chmie, 2016;67(8):1618-1622.

  • 3. Artun FT, Karagoz A, Ozcan G et al. In vitro anticancer and cytotoxic activities of some plant extracts on HeLa and Vero cell lines. J BUON, 2016;21(3):720-5.

  • 4. Cha MR, Park JH, Choi YH et al. Alphaglucosidase inhibitors from the branches extract of Cotinus coggygria. Korean J Pharmacogn, 2009;40(3):229-232.

  • 5. Cragg GM, Kingston DGI, Newman DJ, editors. Anticancer Agents from Natural Products. Boca Raton, FL: Brunner-Routledge Psychology Press, Taylor & Francis Group; 2005.

  • 6. Demirci B, Demirci F, Başer KHC. Composition of the essential oil of Cotinus coggygria Scop. from Turkey. Flavour Frag J, 2003;18:43-44.

  • 7. Duo J, Ying GG, Wang GW, Zhang L. Quercetin inhibits human breast cancer cell proliferation and induces apoptosis via Bcl-2 and Bax regulation. Mol Med Rep, 2012;5(6):1453-1456.

  • 8. Ferrazzano GF, Roberto L, Catania MR et al. Screening and scoring of antimicrobial and biological activities of Italian vulnerary plants against major oral pathogenic bacteria. Evid Based Compl Alternat Med, 2013;2013:316280.

  • 9. Georgieva L, Mihaylova D. Screening of total phenolic content and radical scavenging capacity of Bulgarian plant species. Int Food Res J, 2015;22(1):240-245.

  • 10. Gospodinova Z, Antov G, Christova R, Krasteva M. Extracts from Tanacetum vulgare L. and Cotinus coggygria Scop. exert a lower in vitro inhibitory effect on normal mammary epithelial cell viability than on breast cancer. Anticancer Res Abstracts of the Ninth International Conference of Anticancer Research, 2014;34:5928.

  • 11. Huang KC. The Pharmacology of Chinese Herbs. 2nd Edition. Boca Raton: CRC Press; 1999. 193-194.

  • 12. Ivanova D, Gerova D, Chervenkov T, Yankova T. Polyphenols and antioxidant capacity of Bulgarian medicinal plants. J Ethnopharmacol, 2005;96:145-150.

  • 13. Kawada M, Ohno Y, Ri Y et al. Anti-tumor effect of gallic acid on LL-2 lung cancer cells transplanted in mice. Anticancer Drugs, 2001;12:847-852.

  • 14. Kültür S. Medicinal plants used in Kirklareli Province (Turkey). J Ethnopharmacol, 2007;111:341-364.

  • 15. Marčetić M, Božić D, Milenković M et al. Antimicrobial, antioxidant and anti-inflammatory activity of young shoots of the smoke tree, Cotinus coggygria Scop. Phytother Res, 2013;27:1658-1663.

  • 16. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 1983;65(1-2):55-63.

  • 17. Nićiforović N, Mihailović V, Mašković P et al. Antioxidant activity of selected plant species; potential new sources of natural antioxidants. Food Chem Toxicol, 2010;48:3125-3130.

  • 18. Ploetz KL. An Ethnobotanical Study of Wild Herb Use in Bulgaria [MSc Thesis], Michigan Technological University; 2000.

  • 19. Pollio A, Zarrelli A, Romanucci V Polyphenolic profile and targeted bioactivity of methanolic extracts from Mediterranean ethnomedicinal plants on human cancer cell lines. Molecules, 2016;21(4):395.

  • 20. Riaz T, Abbasi MA, Aziz-ur-Rehman, et al. Cotinus coggyria: A rich source of antioxidants. Pak J Pharm Sci, 2012;25(3):679-686.

  • 21. Ruela-de-Sousa RR, Fuhler GM, Blom N, et al. Cytotoxicity of apigenin on leukemia cell lines: implications for prevention and therapy. Cell Death Dis, 2010;1:e19.

  • 22. Savikin K, Zdunic G, Jankovic T et al. In vitro cytotoxic and antioxidative activity of Cornus mas and Cotinus coggygria. Nat Prod Res, 2009;23(18):1731-1739.

  • 23. Shen Q, Shang D, Ma F, Zhang Z. Pharmacological study on anti-hepatitis effect of Cotinus coggygria Scop. Syrup. Zhongguo Zhong Yao Za Zhi, 1991;16:746-749.

  • 24. Simić M, Vučićević D, Milenković M, Kovačević N. Antioxidant and anti-inflammatory activity of Cotinus coggygria extracts. Planta Med, 2008;74:PA63.

  • 25. Stanojević L, Stanković M, Nikolić V et al. Antioxidant activity and total phenolic and flavonoid contents of Hieracium pilosella L. extracts. Sensors, 2009;9:5702-5714.

  • 26. Vodenicharov D, Petrov A. [Poisonous plants and the poisoning with them]. Sofia–Moscow: Pensoft Publ; 2001. In Bulgarian.

  • 27. Zhang X, Ling Y, Yu H, Ji Y. Studies on mechanism of myricetin-induced apoptosis in human hepatocellular carcinoma HepG-2 cells. Zhongguo Zhongyao Zazhi, 2010;35(8):1046-1050.


Journal + Issues