Evaluation of Fatty Acids Composition of Some Food Samples by Using GC-MS and NMR Techniques

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The purpose of this paper was to compare the composition (weight % of total identified FA) in saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids from 3 food matrices (sunflower oil, palm oil and lard) by 2 different techniques, gas chromatography - mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR). For GC-MS technique, fatty acid methyl esters (FAMEs) identification in the samples was performed by comparison of the retention times (RT) and the mass/charge (m/z) ratio characteristic of each FAME component in the reference standards used (F.A.M.E. Mix C4 - C24 and SRM®2377). FAMEs quantification from food samples was realized by applying correction factors calculated based on reference standards. NMR spectra were recorded on a Bruker Advance 400 MHz spectrometer, operating at 9.4 Tesla corresponding to the resonance frequency of 400.13 MHz for the 1H nucleus. The NMR spectra was recorded directly on the oil without any sample preparation. The difference between the mean values of the fatty acids content determined by GC-MS and NMR was not more than ± 15% for sunflower oil and lard, and ± 6% for palm oil.

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  • Dijkstra A.J. W.W. Christie and G. Knothe. 2007. Nuclear Magnetic Resonance Spectroscopy. The Lipid Handbook 3rd Edition CRC Press New York pp. 455-467.

  • Aued-Pimentel S. M.M.M. Kus E.E. Kumagai V. Ruvieri and O. Zenebon. 2010. Comparison of gas chromatographic and gravimetric methods for quantization of total fat and fatty acids in foodstuffs. Quim. Nova 33(1): 76-84.

  • Chowdhury K. L.A. Banu S. Khan and A. Latif. 2007. Studies on the fatty acid composition of edible oil. Bangladesh Journal of Scientific and Industrial Research 42(3): 311-316.

  • Firl N. H. Kienberger and M. Rychlik. 2014. Validation of the sensitive and accurate quantitation of the fatty acid distribution in bovine milk. International Dairy Journal 35: 139-144.

  • Ionescu (Bordei) N. M. Popescu A. Bratu D. Istrati C. Ott and A. Meghea. 2015. Valuable Romanian vegetable oils and extract with high pharmaco-cosmetic potential. Revista de Chimie Bucharest 66(9): 1267-1272.

  • Kostik V. S. Memeti and B. Bauer. 2013. Fatty acid composition of edible oils and fats. Journal of Hygienic Engineering and Design 4: 112-116.

  • Manzano P. J.C. Diego M.J. Nozal J.L. Bernal and J. Bernal. 2012. Gas chromatography-mass spectrometry approach to study fatty acid profiles in fried potato crisps. Journal of Food Composition and Analysis 28: 31-39.

  • Montoya C. B. Cochard A. Flori D. Cros R. Lopes T. Cuellar S. Espeout I. Syaputra P. Villeneuve M. Pina E. Ritter T. Leroy and N. Billotte. 2014. Genetic architecture of palm oil fatty acid composition in cultivated oil palm (Elaeis guineensis Jacq.) compared to its wild relative E. oleifera (H.B.K) Cortés. PLoS ONE 9(5): 1-13 e95412.

  • Chira N. 2011. Food Fat: Methods of Analysis Authentication (Grăsimile alimentare: Metode de analiză autentificare). Ed. Printech Bucuresti

  • Piasentier E. N. Di Bernardo M. Morgante A. Sepulcri and M. Vitale. 2009. Fatty acid composition of heavy pig back fat in relationship to some animal factors. Italian Journal of Animal Science 8(2): 531-533.

  • Popa M. N. Babeanu G. Dicu A. Teodorescu and N. Boaghe. 2013. The influence of environmental conditions and planting date on sunflower oil content and fatty acids composition. Scientific Bulletin. Series F. Biotechnologies XVIII: 65-68.

  • Popović S. D. Ćolović P. Ikonić T. Tasić L. Kostadinović and J. Lević. 2017. Influence of temperature regime in gas chromatography on polyunsaturated fatty acid determination in Petrovskáklobasá. Romanian Biotechnological Letters 22(4): 12812-12820.

  • Ruiz-Aracama A. E. Goicoechea and M.D. Guillén. 2017. Direct study of minor extra-virgin olive oil components without any sample modification. 1H NMR multisupression experiment. A powerful tool. Food Chemistry 228: 301-314.

  • Simionato J.I. J.C. Garcia G. Tadeu dos santos C.C. Oliveira J.V. Visentainer and N. Evelázio des Souza. 2010. Validation of the determination of fatty acids in milk by gas chromatography. J. Braz. Chem. Soc. 21(3): 520-524.

  • ISO 12966-2:2017. Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters- Part 2: Preparation of methyl esters of fatty acids

  • ISO 12966-4:2015. Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters- Part 4: Determination by capillary gas chromatography

  • SR EN ISO 661:2005. Animal and vegetable fats and oils. Preparation of test sample

  • Tan Z. E. Reyes-Suarez W. Indrasena J.A. Kralovec. 2017. Novel approach to study fish oil oxidation using 1H nuclear magnetic resonance spectroscopy. Journal of Functional Foods 36: 310-316.

  • Vicente J. M. Geraldo de Carvalho and E.E. Garcia- Rojas. 2015. Fatty acids profile of Sacha Inchi oil and blends by 1H NMR and GC-FID. Food Chemistry 181: 215-221.

  • Li Y. and B.A. Watkins. 2001. Current Protocols in Food Analytical Chemistry. Ed. John Wiley and Sons Inc. New York pp. D1.2.1-D1.2.15.

  • Yeboah E.M.O. I. Kobue-Lekalake J.C. Jackson E.N. Muriithi O. Matenanga and S.O. Yeboah. 2017. Application of high resolution NMR FTIR and GC-MS to a comparative study of some indigenous seed oils from Botswana. Innovative Food Science and Emerging Technologies 44: 181-190.

  • Zhang M. X. Yang H.T. Zhao A.J. Dong J. Wang G.Y. Liu P. Wang C.L. Cheng and H. Zhang. 2015. A quick method for routine analysis of C18 trans fatty acids in non-hydrogenated edible vegetable oils by gas chromatography-mass spectrometry. Food Control 57: 293-301.

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