Removal of landfill leachate toxicity and genotoxicity by two treatment methods

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Leachates from active and closed municipal solid waste landfills can be a major source of contamination to groundwater and surface waters. In the present study the toxic and genotoxic potential of leachate from an old sanitary landfill prior to and following chemical and electrochemical treatments were assessed using Lemna, Allium, and comet tests. Photosynthetic pigments, malondialdehyde (indicator of lipid peroxidation) and antioxidant enzyme activities were evaluated as additional indicators of toxicity in duckweed. Following duckweed exposure to 25 % dilution of landfill leachate, growth rate and photosynthetic pigments content significantly decreased while lipid peroxidation increased despite stimulation of antioxidative defence mechanisms. Diluted leachate induced DNA strand breaks in duckweed cells as evidenced by the comet assay. Regarding the Allium test, untreated leachate caused inhibition of Allium cepa cell division and induction of mitotic and chromosomal aberrations. Although both water treatments completely reduced genotoxicity of leachate, the electrochemical method was found to be more efficient in removing toxic substances present in landfill leachate and thus more suitable for treating such leachates prior to their discharge into the environment. As landfill leachates pose a risk to human health and environment in general due to their (geno)toxicity, the present study demonstrates that the ecotoxicity/genotoxicity assays should be used in leachate risk assessment together with physicochemical analysis.

1. Kjeldsen P, Barlaz MA, Rooker AP, Baun A, Ledin A, Christensen TH. Present and long-term composition of MSW landfill Leachate: a review. Crit Rev Environ Sci Technol 2002;32:297-336. doi: 10.1080/10643380290813462

2. Bortolotto T, Bertoldo JB, da Silveira FZ, Manganelli Defaveri T, Silvano J, Pich CT. Evaluation of the toxic and genotoxic potential of landfill leachates using bioassays.Environ Toxicol Pharmacol 2009;28:288-93. doi: 10.1016/j. etap.2009.05.007

3. Chandra S, Chauhan LK, Murthy RC, Saxena PN, Pande PN, Gupta SK. Comparative biomonitoring of leachates from hazardous solid waste of two industries using the Allium test.Sci Total Environ 2005;347:46-52. doi: 10.1016/j. scitotenv.2005.01.002

4. Sang N, Li G, Xin X. Municipal landfill leachate induces cytogenetic damage in root tips of Hordeum vulgare.Ecotoxicol Environ Saf 2006;63:469-73. doi: 10.1016/j. ecoenv.2005.02.009

5. Oreščanin V, Ruk D, Kollar R, Lovrenčić Mikelić I, Nad K, Mikulić N. A combined treatment of landfill leachate using calcium oxide, ferric chloride and clinoptilolite. J Environ Sci Health A Tox Hazard Subst Environ Eng 2011;46:323-8. doi: 10.1080/10934529.2011.539118

6. Oreščanin V, Kollar R, Ruk D, Nad K. Characterization and electrochemical treatment of landfill leachate. J Environ Sci Health A Tox Hazard Subst Environ Eng 2012;47:462-9. doi: 10.1080/10934529.2012.646146

7. Wang W. Ammonia toxicity to macrophytes (common duckweed and rice) using static and renewal methods.Environ Toxicol Chem 1991;10:1173-7. doi: 10.1002/ etc.5620100909

8. Leme DM, Marin-Morales MA. Allium cepa test in environmental monitoring: A review on its application. Mutat Res 2009;682:71-81. doi: 10.1016/j.mrrev.2009.06.002

9. Fiskesjö G. The Allium test as a standard in environmental monitoring. Hereditas 1985;102:99-112. PMID:3988545

10. American Public Health Association (APHA), American Water Works Association (AWWA), Water Environment Federation (WEF). Standard Methods for Examination of Water and Wastewater. 22nd ed. Washington: American Public Health Association; 2012.

11. Oreščanin V, Lovrenčić I, Mikelić L, Lulić S. Applicability of MiniPal 4 compact EDXRF spectrometer for soil and sediment analysis. X-ray Spectrom 2008;37:508-11. doi: 10.1002/xrs.1079

12. ISO/DIS 20079, 2004. International Organization for Standardization / Draft International Standard. Water quality - Determination of the toxic effect of water constituents and wastewater on duckweed (Lemna minor) - Duckweed growth inhibition test.

13. Lichtenthaler HK. Chlorophylls and carotenoids: pigments of photosynthetic membranes. Methods Enzymol 1987;148:350-82. doi: 10.1016/0076-6879(87)48036-1

14. Gichner T, Patková Z, Száková J, Demnerová K. Cadmium induces DNA damage in tobacco roots, but no DNA damage, somatic mutations or homologous recombination in tobacco leaves. Mutat Res 2004;559:49-57. doi: 10.1016/j. mrgentox.2003.12.008

15. Heath RL, Packer L. Photoperoxidation in isolated chloroplasts. I. kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 1968;125:189-98. doi: 10.1016/0003-9861(68)90654-1

16. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54. doi: 10.1016/0003-2697(76)90527-3

17. Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 1981;22:867-80.

18. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6. PMID: 6727660

19. Foo KY, Hameed BH. An overview of landfill leachate treatment via activated carbon adsorption process. J Hazard Mater 2009;171:54-60. doi: 10.1016/j.jhazmat.2009.06.038

20. Pravilnik o graničnim vrijednostima opasnih i drugih tvari u otpadnim vodama [Ordinance on limit values of indicators of hazardous and other substances in wastewater, in Croatian]. Narodne novine 94/2008.

21. Gajski G, Oreščanin V, Garaj-Vrhovac V. Chemical composition and genotoxicity assessment of sanitary landfill leachate from Rovinj, Croatia. Ecotoxicol Environ Saf 2012;78:253-9. doi: 10.1016/j.ecoenv.2011.11.032

22. Kwasniewska J, Nałęcz-Jawecki G, Skrzypczak A, Płaza GA, Matejczyk M. An assessment of the genotoxic effects of landfill leachates using bacterial and plant tests. Ecotoxicol E n v i r o n S a f 2 0 1 2 ; 7 5 : 5 5 - 6 2 . d o i : 1 0 . 1 0 1 6 / j . ecoenv.2011.08.020

23. Gaulden ME. Hypothesis: some mutagens directly alter specific chromosomal proteins (DNA topoisomerase II and peripheral proteins) to produce chromosome stickiness, which causes chromosome aberrations. Mutagenesis 1987;2:357-65. doi: 10.1093/mutage/2.5.357

24. Britto DT, Kronzucker HJ. NH4 + toxicity in higher plants: a critical review. J Plant Physiol 2002;159:567-84. doi: 10.1078/0176-1617-0774

25. Radić S, Stipaničev D, Cvjetko P, Lovrenčić Mikelić I, Marijanović Rajčić M, Širac S, Pevalek-Kozlina B, Pavlica M. Ecotoxicological assessment of industrial effluent using duckweed (Lemna minor L.) as a test organism. Ecotoxicology 2010;19:216-2. doi: 10.1007/s10646-009-0408-0

26. Radić S, Stipaničev D, Cvjetko P, Marijanović Rajčić M, Širac S, Pevalek-Kozlina B, Pavlica M. Duckweed Lemna minor as a tool for testing toxicity and genotoxicity of surface waters. Ecotoxicol Environ Saf 2011;74:182-7. doi: 10.1016/j.ecoenv.2010.06.011

27. Horvat T, Vidaković-Cifrek Ž, Oreščanin V, Tkalec M, Pevalek-Kozlina B. Toxicity assessment of heavy metal mixtures by Lemna minor L. Sci Total Environ 2007;384:229-38. doi: 10.1016/j.scitotenv.2007.06.007

28. Perfus-Barbeoch L, Leonhardt N, Vavasseur A, Forestier C.Heavy metal toxicity: cadmium permeates through calcium channels and disturbs the plant water status. Plant J 2002;32:539-48. doi: 10.1046/j.1365-313X.2002.01442.x

29. Hou W, Chen X, Song G, Wang Q, Chang CC. Effects of copper and cadmium on heavy metal polluted waterbody restoration by duckweed (Lemna minor). Plant Physiol Biochem 2007;45:62-9. PMID: 17300947

30. Zhu Z, Gerendas J, Bendixen R, Schinner K, Tabrizi H, Sattelmacher B, Hansen U-P. Different tolerance to light stress in NO3 - - and NH4 +- grown Phaseolus vulgaris L. Plant Biol 2000;2:558-70. doi: 10.1055/s-2000-7498

31. Fatima RA, Ahmad M. Certain antioxidant enzymes of Allium cepa as biomarkers for the detection of toxic heavy metals in wastewater. Sci Total Environ 2005;346:256-73. PMID: 15993699

32. Widziewicz K, Kalka J, Skonieczna M, Madej P. The comet assay for the evaluation of genotoxic potential of landfill leachate. Sci World J 2012;2012:435239. doi: 10.1100/2012/435239

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