Association Between Glutathione S-Transferase Omega 1 A140D Polymorphism in the Turkish Population and Susceptibility to Non-Small Cell Lung Cancer

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Recent years have seen a growing evidence of ethnic differences in the frequency of glutathione S-transferase omega 1 (GSTO1) A140D gene polymorphism, which is associated with various cancers such as breast and liver. Until now however, no association has been investigated between the GSTO1 A140D polymorphism and lung cancer. The aim of our study was to see if there was one in the Turkish population. To do that, we identified GSTO1 A140D polymorphism in 214 unrelated healthy individuals and 172 patients with non-small cell lung cancer (NSCLC) using the polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method. The frequencies of A/A (wild type), A/D (heterozygous mutant), and D/D (homozygous mutant) GSTO1 A140D genotypes in healthy subjects were 48 %, 41 %, and 11 %, respectively. In NSCLC patients they were 48 %, 45 %, and 7 %, respectively. We found no significant association between the GSTO1 A140D gene polymorphism and NSCLC or its histological subtypes, namely squamous cell carcinoma or adenocarcinoma. Furthermore, this polymorphism did not correlate with smoking. Our study is the first to show that the frequency of GSTO1 A140D gene polymorphism in the Turkish population is similar to other Caucasian populations and that this polymorphism is not associated with susceptibility to NSCLC.

1. Autrup H. Gene-environment interactions in environmental carcinogens. In: Nicolopoulou-Stamati P, Hens L, Howard CV, Van Larebeke N, editors. Cancer as an environmental disease. Dordrecht: Kluwer Academic Publisher; 2004. p. 71-101.

2. Strange RC, Spiteri MA, Ramachandran S, Fryer AA. Glutathione-S-transferase family of enzymes. Mutat Res 2001;482:21-6.

3. Yin ZL, Dahlstrom JE, Le Couteur DG, Board PG. Immunohistochemistry of omega class glutathione Stransferase in human tissues. J Histochem Cytochem 2001;49:983-7. doi: 10.1177/002215540104900806

4. Harju TH, Peltoniemi MJ, Rytila PH, Soini Y, Salmenkivi KM, Board PG, Ruddock LW, Kinnula VL. Glutathione STable transferase omega in the lung and sputum supernatants of COPD patients. Respir Res 2007;8:48. doi:10.1186/1465-9921-8-48

5. Dulhunty A, Gage P, Curtis S, Chelvanayagam G, Board P. The glutathione transferase structural family includes a nuclear chloride channel and a ryanodine receptor calcium release channel modulator. J Biol Chem 2001;276:3319-23. doi: 10.1074/jbc.M007874200

6. Wang L, Xu J, Ji C, Gu S, Lv Y, Li S, Xu Y, Xie Y, Mao Y. Cloning, expression and characterization of human glutathione S-transferase Omega 2. Int J Mol Med 2005;16:19-27.

7. Board PG. The omega-class glutathione transferases: structure, function, and genetics. Drug Metab Rev 2011;43:226-35. doi: 10.3109/03602532.2011.561353

8. Tanaka-Kagawa T, Jinno H, Hasegawa T, Makino Y, Seko Y, Hanioka N, Ando M. Functional characterization of two variant human GSTO 1-1s (Ala140Asp and Thr217Asn). Biochem Biophys Res Commun 2003;301:516-20.

9. Whitbread AK, Tetlow N, Eyre HJ, Sutherland GR, Board PG. Characterization of the human Omega class glutathione transferase genes and associated polymorphisms. Pharmacogenetics 2003;13:131-44. doi: 10.1097/01. fpc.0000054062.98065.6e

10. Board PG, Anders MW. Glutathione transferase omega 1 catalyzes the reduction of S-(phenacyl)glutathiones to acetophenones. Chem Res Toxicol 2007;20:149-54. doi: 10.1021/tx600305y

11. Granja F, Morari EC, Assumpcao LV, Ward LS. GSTO polymorphism analysis in thyroid nodules suggest that GSTO1 variants do not influence the risk for malignancy. Eur J Cancer Prev 2005;14:277-80.

12. Ozturk A, Desai PP, Minster RL, Dekosky ST, Kamboh MI. Three SNPs in the GSTO1, GSTO2 and PRSS11 genes on chromosome 10 are not associated with age-at-onset of Alzheimer’s disease. Neurobiol Aging 2005;26:1161-5. doi: 10.1016/j.neurobiolaging.2004.11.001

13. Marahatta SB, Punyarit P, Bhudisawasdi V, Paupairoj A, Wongkham S, Petmitr S. Polymorphism of glutathione Stransferase omega gene and risk of cancer. Cancer Lett 2006;236:276-81. doi: 10.1016/j.canlet.2005.05.020

14. Kolsch H, Linnebank M, Lutjohann D, Jessen F, Wullner U, Harbrecht U, Thelen KM, Kreis M, Hentschel F, Schulz A, von Bergmann K, Maier W, Heun R. Polymorphisms in glutathione S-transferase omega-1 and AD, vascular dementia, and stroke. Neurology 2004;63:2255-60. doi: 10.1212/01.WNL.0000147294.29309.47

15. Wahner AD, Glatt CE, Bronstein JM, Ritz B. Glutathione S-transferase mu, omega, pi, and theta class variants and smoking in Parkinson’s disease. Neurosci Lett 2007;413:274-8. doi: 10.1016/j.neulet.2006.11.053

16. Fu S, Wu J, Chen F, Sun D, Fu S. Polymorphisms of Glutathione S-transferases Omega-1 among ethnic populations in China. BMC Genet 2008;9:29. doi: 10.1186/1471-2156-9-29

17. Takeshita H, Fujihara J, Takastuka H, Agusa T, Yasuda T, Kunito T. Diversity of glutathione s-transferase omega 1. A140D; and 2. N142D; gene polymorphisms in worldwide populations. Clin Exp Pharmacol Physiol 2009;36:283-6. doi: 10.1111/j.1440-1681.2008.05058.x

18. Polimanti R, Piacentini S, Porreca F, Fuciarelli M. Glutathione S-transferase ω class. GSTO; polymorphisms in a sample from Rome (Central Italy). Ann Hum Biol 2010;37:585-92. doi: 10.3109/03014460903508520

19. Kunak SC, Ada AO, Karacaoglan V, Soydas E, Bilgen S, Iscan M. Drug/xenobiotic metabolizing enzyme polymorphisms in a Turkish population. Afr J Pharm Pharmacol 2012;6:2068-74. doi: 10.5897/AJPP12.339

20. Peter H, Deutschmann S, Reichel C, Hallier E. Metabolism of methyl chloride by human erythrocytes. Arch Toxicol 1989;63:351-5. doi: 10.1007/BF00303122

21. Garte S, Gaspari L, Alexandrie AK, Ambrosone C, Autrup H, Autrup JL, Baranova H, Bathum L, Benhamou S, Boffetta P, Bouchardy C, Breskvar K, Brockmoller J, Cascorbi I, Clapper ML, Coutelle C, Daly A, Dell’Omo M, Dolzan V, Dresler CM, Fryer A, Haugen A, Hein DW, Hildesheim A, Hirvonen A, Hsieh LL, Ingelman-Sundberg M, Kalina I, Kang D, Kihara M, Kiyohara C, Kremers P, Lazarus P, Le Marchand L, Lechner MC, van Lieshout EM, London S, Manni JJ, Maugard CM, Morita S, Nazar-Stewart V, Noda K, Oda Y, Parl FF, Pastorelli R, Persson I, Peters WH, Rannug A, Rebbeck T, Risch A, Roelandt L, Romkes M, Ryberg D, Salagovic J, Schoket B, Seidegard J, Shields PG, Sim E, Sinnet D, Strange RC, Stucker I, Sugimura H, To-Figueras J, Vineis P, Yu MC, Taioli E. Metabolic gene polymorphism frequencies in control populations. Cancer Epidemiol Biomarkers Prev 2001;10:1239-48.

22. Aktas D, Ozen H, Atsu N, Tekin A, Sozen S, Tuncbilek E. Glutathione S-transferase M1 gene polymorphism in bladder cancer patients. A marker for invasive bladder cancer? Cancer Genet Cytogenet 2001;125:1-4.

23. Olsen A, Autrup H, Sorensen M, Overvad K, Tjonneland A. Polymorphisms of glutathione S-transferase A1 and O1 and breast cancer among postmenopausal Danish women. Eur J Cancer Prev 2008;17:225-9. doi: 10.1097/ CEJ.0b013e3282b6fe1e

24. Pongstaporn W, Pakakasama S, Sanguansin S, Hongeng S, Petmitr S. Polymorphism of glutathione S-transferase Omega gene: association with risk of childhood acute lymphoblastic leukemia. J Cancer Res Clin Oncol 2009;135:673-8. doi: 10.1007/s00432-008-0501-4

25. Wang YH, Yeh SD, Shen KH, Shen CH, Juang GD, Hsu LI, Chiou HY, Chen CJ. A significantly joint effect between arsenic and occupational exposures and risk genotypes/ diplotypes of CYP2E1, GSTO1 and GSTO2 on risk of urothelial carcinoma. Toxicol Appl Pharmacol 2009;241:111-8. doi: 10.1016/j.taap.2009.08.008

26. Dubey S, Powell CA. Update in lung cancer 2007. Am J Respir Crit Care Med 2008;177:941-6. doi: 10.1164/ rccm.200801-107UP

27. Yanbaeva DG, Wouters EF, Dentener MA, Spruit MA, Reynaert NL. Association of glutathione-S-transferase omega haplotypes with susceptibility to chronic obstructive pulmonary disease. Free Radic Res 2009;43:738-43. doi: 1 0.1080/10715760903038440

28. Hu YC, Sidransky D, Ahrendt SA. Molecular detection approaches for smoking associated tumors. Oncogene 2002;21:7289-97. doi: 10.1038/sj.onc.1205805

29. Chan EC, Lam SY, Fu KH, Kwong YL. Polymorphisms of the GSTM1, GSTP1, MPO, XRCC1, and NQO1 genes in Chinese patients with non-small cell lung cancers: relationship with aberrant promoter methylation of the CDKN2A and RARB genes. Cancer Genet Cytogenet 2005;62:10-20.

30. Hoffmann D, Rivenson A, Murphy SE, Chung F-L, Amin S, Hecht SS. Cigarette smoking and adenocarcinoma of the lung: the relevance of nicotine-derived N-nitrosamines. J Smoking Rel Disord 1993;4:165-90.

31. Denissenko MF, Pao A, Tang M-S, Pfeifer GP. Preferential formation of benzo[a]pyrene adducts at lung cancer mutational hotspots in p53. Science 1996;274:430-2. doi: 10.1126/science.274.5286.430

32. Ada AO, Kunak SC, Hancer F, Bilgen S, Suzen SH, Alpar S, Gulhan M, Kurt B, Iscan M. CYP and GST polymorphisms and survival in advanced non-small cell lung cancer patients. Neoplasma 2010;57:512-21.

33. Ada AO, Kunak SC, Hancer F, Soydas E, Alpar S, Gulhan M, Iscan M. Association between GSTM1, GSTT1, and GSTP1 polymorphisms and lung cancer risk in a Turkish population. Mol Biol Rep 2012;39:5985-93. doi: 10.1007/ s11033-011-1411-0

34. Breslow NE, Day NE, editors. Statistical Methods in Cancer Research. Volume I - The Analysis of Case-Control Studies. IARC Sci Publ No. 32. Lyon: IARC; 1980.

35. Ulusoy G, Arinc E, Adali O. Genotype and allele frequencies of polymorphic CYP2E1 in the Turkish population. Arch Toxicol 2007;81:711-8. doi: 10.1007/s00204-007-0200-y

36. Chariyalertsak S, Purisa W, Sangrajrang S. Role of glutathione S-transferase omega gene polymorphisms in breast-cancer risk. Tumori 2009;95:739-43. doi: 10.1700/467.5515

37. Andonova IE, Justenhoven C, Winter S, Hamann U, Baisch C, Rabstein S, Spickenheuer A, Harth V, Pesch B, Bruning T, Ko YD, Ganev V, Brauch H. No evidence for glutathione S-transferases GSTA2, GSTM2, GSTO1, GSTO2, and GSTZ1 in breast cancer risk. Breast Cancer Res Treat 2010;121:497-502. doi: 10.1007/s10549-009-0589-5

38. Schneider J, Bernges U, Philipp M, Woitowitz HJ. GSTM1, GSTT1, and GSTP1 polymorphism and lung cancer risk in relation to tobacco smoking. Cancer Lett 2004;208:65-74. doi: 10.1016/j.canlet.2004.01.002

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