Genetic Diversity in Domestic and Introduced Wheats

Open access

Abstract

A set of 33 wheat EST-SSR markers was designed and 18 from them were polymorphic and used for assessment of genetic diversity within 36 introduced genotypes of hexaploid bread wheat. Altogether 105 alleles were detected, in average 3.18 alleles per locus. Maximum number of alleles 14 was detected at the locus TDI389708. Five the most polymorphic markers were used for the evaluation and comparison of genetic variation within 46 domestic (Slovak) wheat genotypes and 36 introduced (foreign) wheat genotypes. The number of alleles per used primer pair within domestic genotypes varied from 7 to 19, with an average of 13.2 alleles, an average gene diversity 0.846 and PIC 0.980 per locus. The number of alleles per primer within introduced genotypes varied from 7 to 14, with an average of 10.8 alleles, an average gene diversity 0.780 and PIC 0.958 per locus. The level of polymorphism in EST- SSRs was sufficient for discrimination between genotypes and variation within domestic genotypes was slightly higher than in introduced genotypes. Variation revealed by 5 selected EST-SSR markers clustered genotypes according to origin. Domestic and introduced wheats were grouped distinctly into two separate groups.

AKHUNOV, E.D. - AKHUNOV, E.D. - GOODYEAR, A.V. - GENG, S. - QI, L.-L. - ECHALIER, B. - GILL, B.S. - MIFTAHUDIN - GUSTAFSON, J.P. - LAZO, G. - CHAO, S. - ANDERSON, O.D. - LINKIEWICZ, A.M. - DUBCOVSKY, J. - LA ROTA, M. - SORRELLS, M.E. - ZHANG, D. - NGUYEN, H.T. - KALAVACHARLA, V. - HOSSAIN, V. - KIANIAN, S.F. - PENG, J. - LAPITAN, N.L.V. - GONZALEZ- HERNANDEZ, J.L. - ANDERSON, J.A. - CHOI, D.-W. - CLOSE, T.J. - DILBIRLIGI, M. - GILL, K.S. - WALKER-SIMMONS, M.K. - STEBER, C. - MCGUIRE, P.E. - QUALSET, C.O. - DVORAK, J. 2003. The organization and rate of evolution of wheat genomes are correlated with recombination rates along chromosome arms. In Genome Research, vol. 13, pp.753-763. DOI :10.1101/gr.808603.

AKKAYA, M.S. - BUYUKUNAL-BAL, E.B. 2004. Assessment of genetic variation of bread wheat varieties using microsatellite markers. In Euphytica, vol, 135, pp. 179-185. DOI: 10.1023/B:EU PH.0000014908.02499.41.

BASSAM, B.J. - CAETANO-ANOLLES, G. - GRESSHOFF, P.M. 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. In Analytical Biochemistry, vol. 196, no. 1, pp. 80-83. DOI: 10.1016/0003-2697(91)90120-I.

CORDEIRO, G.M. - CASU, R. - McINTYRE, C.L. - MANNERS, J.M. - HENRY, R.J. 2001. Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to Erianthus and sorghum. In PlantScience, vol. 160, pp. 1115-1123. DOI: 10.1016/ S0168-9452(01)00365-X.

EUJAYL, I. - SORRELLS, M.E. - BAUM, M. - WOLTERS, P. - POWELL, W. 2001. Assessment of genotypic variation among cultivated durum wheat based on EST-SSRs and genomic-SSRs. In Euphytica, vol. 119, pp. 39-43.

FU, Y.B. - PETERSON, G.W. - GAO, J.K.Y.L. - JIA, J. - RICHARDS, K.W. 2006. Impact of plant breeding on genetic diversity of the Canadian hard red spring wheat germplasm as revealed by EST-derived SSr markers. In Theoretical and Applied Genetics, vol. 112, pp. 1239-1247. DOI: 10.1007/s00122-006-0225-2.

FUJITA, Y. - FUKUOKA, H. - YANO, H. 2009. Identification of wheat cultivars using EST-SSR markers. In Breeding Science, vol. 59, pp. 159-167. DOI: 10.1270/jsbbs.59.159.

GADALETA, A. - GIANCASPRO, A. - GIOVE, S.L. - ZACHEO, S. - MANGINI, G. - SIMEONE, R. - SIGNORILE, A. - BLANCO, A. 2009. Genetic and physical mapping of new EST-derived SSRs on the A and B genome chromosomes of wheat. In Theoreticaland applied genetics, vol. 118, pp. 1015-1025. DOI: 10.1007/s00122-008-0958-1.

GADALETA, A. - GIANCASPRO, A. - ZACHEO, S. - NIGRO, D. - GIOVE, S.L. - COLASUONNO, P. - BLANCO, A. 2011. Comparison of genomic and EST-derived SSR markers in phylogenetic analysis of wheat. In Plant Genetic Resources, vol. 9, pp. 243-246. DOI:10.1017/S14792621 1100030X.

GREGÁŇOVÁ, Ž. - KRAIC, J. - GÁLOVÁ, Z. 2005. Effectiveness of microsatellites in differentiation of elite wheat cultivars. In Biologia, vol. 60, pp. 665-670.

GREGÁŇOVÁ, Ž. - VIVODÍK, M. - GÁLOVÁ, Z. 2008. Analysis of diversity of wheat by microsatellites. In Biotechnology 2008 [CD-ROM]. České Budějovice : University of South Bohemia České Budějovice, pp. 109-1 11. ISBN 80-85645-58-0.

GUPTA, P.K. - RUSTGI, S. - SHARMA, S. - SINGH, R. - KUMAR, N. - BALYAN, H. S. 2003. Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat. In MolecularGenetics and Genomics, vol. 270, pp. 315-323. DOI: 10.1007/s00438-003-0921-4.

HUANG, X.Q. - BORNER, A. - RODER, M.S. - GANAL, M. 2002. Assessing genetic diversity of wheat (Triticumaestivum L.) germplasm using microsatellite markers. In Theoretical and Applied Genetics, vol. 105, pp. 699-707. DOI: 10.1007/s00122-002-0959-4.

KANTETY, R.V. - LA ROTA, M. - MATTHEWS, D.E. - SORRELLS, M.E. 2002. Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat. In Plant Molecular Biology, vol. 48, pp. 501-510. DOI: 10.1023/A:1014875206165.

LI, Y.CH. - KOROL, A.B. - FAHIMA, T. - NEVO, E. 2004. Microsatellites within genes: structure, function and evolution. In Molecular Biology and Evolution, vol. 21, pp. 991-1007. DOI: 10.1093/molbev/msh073.

PAETKAU, D. - CALVERT, W. - STIRLING, I. - STROBECK, C. 1995. Microsatellite analysis of population structure in Canadian polar bears. In: Molecular Ecology, vol. 4, pp. 347-354. DOI: 10.1 111/j.1365-294X.1995.tb00227.x.

PRASAD, M. - VARSHNEY, R.K. - ROY, J.K. - BALYAN, H.S. - GUPTA, P.K. 2000. The use of microsatelites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. In Theoreticaland Applied Genetics, vol. 100, pp. 584-592. DOI: 10.1007/s001220050077.

ROZEN, S. - SKALETSKY, H. 2000. Primer3 on the www for general users and for biologist programmers. In MISENER, S. - CRAWETZ S.A. (eds.) BioinformaticsMethods and Protocols. Methods in MolecularBiology, vol. 132, pp. 365 -386.

SONG, Z.P. - JIANG, X.B. - ZHANG, M. - WANG, Z.L. - BO, W.H. - AN, X.M. - ZHANG, D.Q. - ZHANG, Z.Y. 2012. Differences of EST-SSR and genomic- SSR markers in assessing genetic diversity in poplar. In Forestry Studies in China, vol. 14, pp. 1-7. DOI: 10.1007/s1 1632-012-0106-5.

WANG, H.Y. - WEI, Y.M. - YAN, Z.H. - ZHENG, Y.L. 2007. EST-SSR DNA polymorphism in durum wheat (Triticum durum L.) collections. In Journalof Applied Genetics, vol. 48, no. 1, pp. 35-42. DOI: 10.1007/BF03194655.

WEBER, J.L. 1990. Informativeness of human (dC-dA)n. (dG-dT)n polymorphism. In Genomics, vol. 7, pp. 524 -530. DOI: 10.1016/0888-7543(90)90195-Z.

WEIR, B.S. 1990. Genetic data analysis. II. Methods fordiscrete population genetic data. 2nd ed. Sinauer Associates : Sunderland, Mass., p. 370.

YANG, X.Q. - LIU, P. - HAN, Z.F. - NI, Z.F. - LIU, W.Q. - SUN, Q.X. 2005. Comparative analysis of genetic diversity revealed by genomic-SSR, EST‒SSR and pedigree in wheat (Triticum asetivum L.). In ActaGenetica Sinica, vol. 32, pp. 406-416.

YU, J.-K. - DAKE, T.M. - SINGH, S. - BENSCHER, D. - LI, W.L. - GILL, B. - SORRELLS, M.E. 2004. Development and mapping of EST-derived simplesequence repeat markers for hexaploid wheat. In Genome, vol. 47, pp. 805-818. DOI: 10.1 139/g04-057.

ZHUANG, P.P. - REN, Q. - LI, W. - CHEN, G.Y. 2011. Genetic diversity of Persian wheat (Triticumturgidum ssp. carthlicum) accessions by EST‒ SSR markers. In American Journal of Biochemistryand Molecular Biology, vol. 1, pp. 223-230. DOI: 10.3923/ajbmb.201 1.223.230.

Agriculture (Pol'nohospodárstvo)

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