Methodological Approaches to Simple Enzyme Polymorphism Analyses of Amaranth Species (Amaranthus SP.)

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Methodological Approaches to Simple Enzyme Polymorphism Analyses of Amaranth Species (Amaranthus SP.)

Amaranths have drawn a great interest in last years as an agronomic crop, alternative energy source and ornamental plants around the world. Several of them are cultivated as the leaf vegetables, cereals, or as the colourful, quick growing garden plants. Increasing demand for the breeding and proteomic study in Amaranthus species led us to test some methodological approaches to enzyme polymorphism analysis. For these experiments genotypes of Amaranthus cruentus L. (genotype Ficha) and mutant line of hybrid K-433 were selected, characterized by a good seed quality and quantity, suitable for food production. The attention was devoted to test of published methodology, which were slightly modified for enzyme (ACP, ADH, CAT, DIA, GOT, IDH, MDH, PGD, PGI and PGM) multiplicity analysis for amaranths and testing of feasibility of chosen analysed organ weights, dimensions of Whatman No. 2 wicks and different volumes of extract buffer. Results from enzyme multiplicity analysis are presented by means of photographed fingerprints and phenotypes are expressed in diagrams where positions of zymograme bands are marked by using factors of relative mobility (Rm).

BOURGOIN-GRENECHE, M. - LALLEMAND, J. 1993. Electrophoresis and its application to the description of varieties. GEVES - La Miniere - F 78285 GUYANCOURT Cedex, France, 1993, 63 pp.

BOURGOIN-GRENECHE, M. - LALLEMAND, J. - POUGET, R. 1998. Technical reference manual for the isoenzymatic analysis of maize. GEVES - La Miniére - F 78285 GUYANCOURT Cedex, France, 1998. 73 pp. ISBN 2-910171-28-0.

CARDY, B.J. - STUBER, C.W. - GOODMAN, M.M. 1980. Techniques for starch gel electrophoresis of enzymes from maize (Zea mays L.). Institute of Statistics Mimeograph Series, North Carolina State University, Raleigh, USA, 1980, 87 pp.

CHAN, K.F. - SUN, M. 1997. Genetic diversity and relationships detected by isozyme and RAPD analysis of crop and wild species of Amaranthus. In Theoretical and Applied Genetics, vol. 95, 1997, no. 5-6, pp. 865-873.

ENGELHARDT, M. - BEŽO, M. 2000. Analýza izoenzýmov mnohovýhonkovej kultúry peľovosterilných línií a ich fertilných analógov repy cukrovej (Beta vulgaris L.) [Isosyme analysis of multishoot culture of cytoplasmic male sterility lines and Otypes of sugarbeet (Beta vulgaris L.)]. In Acta fytotechnica et zootechnica, vol. 3, 2000, no. 2, pp. 46-48.

ENGELHARDT, M. - MÚDRY, P. 1999. Identifikácia peľovo sterilných línií a ich fertilných analógov repy cukrovej (Beta vulgaris L.) pomocou izoenzýmových systémov [Identification of cytoplasmic male sterility lines and O-types of sugarbeet (Beta vulgaris L.) by means of isoenzyme systems]. In Poľnohospodárska výroba a skúšobníctvo, vol. 7, 1999, no. 2, pp. 29-32.

GAJDOŠOVÁ, A. - LIBIAKOVÁ, G. - OSTROLUCKÁ, M.G. - FEJÉR, J. 2008. Mutation breeding in selected Amaranthus ssp. In "Amaranth - plant for the future"- 5th International Symposium of the European Amaranth Association, (G. Libiaková, A. Gajdošová, Eds.) Nitra (Slovakia), 2008. ISBN 978-80-89088-70-6, pp. 93-94.

GOODMAN, M.M. - STUBER, C.W. - LEE, C.N. - JOHNSON, F.M. 1980. Genetic control of malate dehydrogenase isozymes in maize. In Genetics, vol. 94, 1980, no. 1, pp. 153-168.

GOPTSIY, T. - VORONCOV, N. - POPOV, V. - ZHYRAVEL, D. - GROMENKO, S. 2008. Grain varieties of amaranth developed by selection at Kharkiv National Agrarian University and the perspectives of their use. In "Amaranth - plant for the future" - 5th International Symposium of the European Amaranth Association, (G. Libiaková, A. Gajdošová, Eds.) Nitra (Slovakia), 2008. ISBN 978-80-89088-70-6, pp. 97-100.

GRENECHE, M. - GIRAUD, G. 1989. Manuel technique de référence pour ľ analyse isoenzymatique du Mais. GEVES - La Miniére - Cedex, Surgeres, 1989, France, 78 pp.

HAUPTLI, H. - JAIN, S. 1984. Allozyme variation and evolutionary relationships of grain amaranths (Amaranthus spp.). In Theoretical and Applied Genetics, vol. 69, 1984, no. 2, pp. 153-165.

JACOBSEN, S.E. - MUJICA, A. 2003. The genetic resources of Andean grain amaranths (Amaranthus caudatus L., A. cruentus L. and A. hypochondriacus L.) in America. In Plant Genetic Resources Newsletter, 2003, no. 133, pp. 41-44.

JAIN, S.K. - WU, L. - VAIDYA, K.R. 1980. Levels of morphological and allozyme variation in Indian amaranths: a striking contrast. In The Journal of Heredity, vol. 71, 1980, no. 4, pp. 283-285.

JEBARA, S. - DREVON, J.J. - JEBARA, M. 2010. Modulation of symbiotic efficiency and nodular antioxidant enzyme activities in two Phaseolus vulgaris genotypes under salinity. In Acta Physiologiae plantarum, vol. 32, 2010, no. 5, pp. 925-932.

KIRKPATRICK, B.A. 1995. Interspecific relationships within the genus Amaranthus (Amaranthaceae). Ph.D. diss. Texas Univ., College Station, TX, USA, 1995, 188 pp.

MAGBANUA, Z.V. - DE MORAES, C.M. - BROOKS, T.D. - WILLIAMS, W.P. - LUTHE, D.S. 2007. Is catalase activity one of the factors associated with maize Resistance to Aspergillus flavus? In The American Phytopathological Society, vol. 20, 2007, no. 6, pp. 697-706.

MANGAL, M. - SHARMA, D.R. 2002. In vitro mutagenesis and cell selection for the induction of black rot resistance in cauliflower. In Journal of Horticultural Science & Biotechnology, vol. 77, 2002, no. 3, pp. 268-272.

MARKERT, C.L. - MOLLER, F. 1959. Multiple forms of enzymes: tissue ontogenetic, and species specific patherns. In The Proceedings of the National Academy of Sciences, vol. 45, 1959, no. 5, pp. 753-763.

MÚDRY, P. - DRAGÚŇ, M. 2007. Effect of cadmium on the enzyme polymorphism of some oxidoreductases in different maize (Zea mays L.) organs. In "Industrial Toxicolog '07" - 27th International Symposium, (V. Koprda, A. Manová, F. Čacho, Eds.), Bratislava (Slovakia), 2007. ISBN 978-80-227-2654-2, pp. 348-353.

MÚDRY, P. - JURÁČEK, Ľ. 1998. Popis a rozlíšenie odrôd slnečnice. Metodické postupy separácie izoenzýmov ACP, GOT, MDH, ME, PGI, PGM a SKD druhu slnečnica ročná (Helianthus annuus L.) [Description and distinguishing of sunflower genotypes. Methodological procedures of isoenzymes separation of ACP, GOT, MDH, ME, PGI, PGM and SKD of sunflower (Helianthus annuus L.)]. In Poľnohospodárska výroba a skúšobníctvo, vol. 6, 1998, no. 4, pp. 32-36.

MÚDRY, P. - JURÁČEK, Ľ. 1999a. Metodologické postupy separácie izoenzýmov IDH, a PGD druhu hrach siaty (Pisum sativum L.) [Methodological procedures of isoenzymes separation of IDH and PGD of pea (Pisum sativum L.)]. In Poľnohospodárska výroba a skúšobníctvo, vol. 7, 1999, no. 1, pp. 32-34.

MÚDRY, P. - JURÁČEK, Ľ. 1999b. Metodické postupy separácie izoenzýmov ACP, DIA, IDH, MPI, PGD, PGM a PRX druhu sója fazuľová (Glycine max. [L.] Merr.) [Methodological procedures of isoenzymes separation of ACP, DIA, IDH, MPI, PGD, PGM and PRX of soybean species (Glycine max. [L.] Merr.)]. In Poľnohospodárska výroba a skúšobníctvo, vol. 7, 1999, no. 2, pp. 26-29.

MÚDRY, P. - JURÁČEK, Ľ. 2001. Modifikovaná štandardizovaná metodika analýzy polymorfizmu jedenástich druhov enzýmov - molekulárnych značkovačov kukurice siatej (Zea mays L.) [Modified and standardized methodology of polymorphism analysis of eleven enzymes - molecular markers of maize (Zea mays L.)]. In Biotechnologické metódy v šľachtení rastlín, BIOS, Nitra: SPU, 2001. ISBN 80-7137-915-8, pp. 68-73.

MÚDRY, P. - JURÁČEK, Ľ. - GÁBORČÍK, N. 1998. Polymorphism of selected isoenzymes in evaluation of identity and homogeneity of chickpea and vetchling genotypes. In Rostlinnávýroba, vol. 44, 1998, no. 3, pp. 103-109.

NEWTON, K.J. - SCHWARTZ, D. 1980. Genetic basis of the major malate dehydrogenase isozymes in maize. In Genetics, vol. 95, 1980, no. 2, pp. 425-442.

PRATT, D.B. - CLARK, L.G. 2001. Amaranthus rudis and A. tuberculatus - one species or two? In Journal of the Torrey Botanical Society, vol. 128, 2001, no. 3, pp. 282-296.

UVÁČKOVÁ, Ľ. - MÚDRY, P. - OBERT, B. - PREŤOVÁ, A. 2008. Enzyme fingerprint analyses in tissue regenerated from anther culture of maize. In Acta Physiologiae Plantarum, vol. 30, 2008, no. 6, pp. 779-785.

SAWHNEY, S. - BASRA, A.S. - KOHLI, R.K. 1981. Enzyme activity and electrophoretic pattern of isoenzymes of peroxidase, esterase and alkaline and acid phosphatase in relation to flowering in Amaranthus viridis L. - a quantitative SD plant. In Biologia Plantarum, vol. 23, 1981, no. 5, pp. 335-341.

STUBER, C.W. - WENDEL, J.F. - GOODMAN, M.M. - SMITH, J.S. C. 1988. Techniques and scoring procedures for starch gel electrophoresis of enzymes from maize (Zea mays L.). In Technical Bulletin, 1988, North Carolina Agricultural Research Service, Raleigh, no. 286, pp. 1-87.

WARWICK, S.I. - BLACK, L.D. 1986. Electrophoretic variation in triazine - resistant and susceptible populations of Amaranthus retroflexux L. In New Phytologist, vol. 104, 1986, no. 5, pp. 661-670.

YUDINA, R.S. - IBRAGIMOVA, S.S. - ZHELEZNOVA, N.B. 2008. Izučenie struktury populjacij amaranta (Amaranthus L.) po izofermentnym lokusam [Structure of amaranth (Amaranthus L.) populations according to isoenzyme alleles]. In Vestnik VOGiS, vol. 12, 2008, no. 3, pp. 385-391.

YUDINA, R.S. - ZHELEZNOVA, N.B. - ZAKHAROVA, O.V. - ZHELEZNOV, A.V. - SHUMNY, V. K. 2005. Isozyme analysis in a genetic collection of amaranths (Amaranthus L.). In Rusian Journal of Genetics, vol. 41, 2005, no. 12, pp. 1395-1400.

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