Voluntary exercise and testosterone therapy caused increase in percentage of Myh6 and expression of oxidative stress marker Cybb in left ventricles of rats

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Abstract

Aim: The aim of this study is to identify a possible damage to heart ventricles caused by supraphysiological doses of testosterone, voluntary physical activity or their combination.

Methods: In the 8-week long experiment, 10-12 weeks old male Wistar rats were administered testosterone depot in dose of 100 mg/kg (TES, n = 15) or vehiculum (CON, n = 12) once a week subcutaneously. Next groups injected with testosterone (SPOTES, n = 12) or vehiculum (SPO, n = 12) were running in exercise wheels ad libitum. Gene expressions in left and right ventricles of the heart were measured by quantitative reverse transcription polymerase chain reaction method.

Results:ln left ventricles of the testosterone groups, we observed a mild but significant increase in the percentage of Myh6 myosin heavy chain isoform and higher expression of NADPH oxidase subunit Cybb (*p < 0.05).

Conclusions:Testosterone affected the expression of genes related to contractile apparatus and oxidative stress in the left ventricle but not in right ventricle of the heart of rats. The observed level of physical activity did not have a compelling effect on the expression of measured genes.

[1] Altamirano F, Oyarce C, Silva P, Toyos M, Wilson C, Lavandero S, et al. Testosterone induces cardiomyocyte hypertrophy through mammalian target of rapamycin complex 1 pathway. Journal of Endocrinology 2009;202:299-307.

[2] Baggish AL and Wood MJ. Athlete’s Heart and Cardiovascular Care of the Athlete: Scientific and Clinical Update. Circulation 2011;123:2723-2735.

[3] Emer E, Yildiz O, Seyrek M, Demirkol S, Topal T, Kurt B, et al. High-dose testosterone and dehydroepiandrosterone induce cardiotoxicity in rats: Assessment of echocardiographic, morphologic, and oxidative stress parameters. Human and Experimental Toxicology 2016;Vol. 35(5): 562-572.

[4] Finocchiaro G, Papadakis M, Robertus JL, et al. Etiology of Sudden Death in Sports. Journal of the American College of Cardiology 2016;67:2108-2115.

[5] de Groote P. Right ventricular systolic function in heart failure: A long story but still the same question. Archives of Cardiovascular Diseases 2016;109: 227-230.

[6] Hydock DS, Lien CY, Schneider CM, Hayward R. Effects of voluntary wheel running on cardiac function and myosin heavy chain in chemically gonadectomized rats. Am J Physiol Heart Circ Physiol 2007;293:H3254-H3264.

[7] Korte FS, Herron TJ, Rovetto MJ, McDonald KS. Power output is linearly related to MyHC content in rat skinned myocytes and isolated working hearts. Am J Physiol Heart Circ Physiol 2005;289:H801-H812.

[8] Lowes BD, Minobe W, Abraham WT, et al. Changes in gene expression in the intact human heart. Downregulation of alpha-myosin heavy chain in hypertrophied, failing ventricular myocardium. J Clin Invest 1997;100(9):2315-24.

[9] Myers J. Exercise and Cardiovascular Health. Circulation 2003;107:e2-e5.

[10] Pongkan W, Chattipakorn SC and Chattipakorn N. Chronic Testosterone Replacement Exerts Cardioprotection against Cardiac Ischemia-Reperfusion Injury by Attenuating Mitochondrial Dysfunction in Testosterone-Deprived Rats. PLoS ONE 2015;10(3):e0122503.

[11] Powers SK and Jackson MJ. Exercise-Induced Oxidative Stress: Cellular Mechanisms and Impact on Muscle Force Production. Physiol Rev 2008;88(4):1243-12764.

[12] Pugh PJ, Jones RD, West JN, Jones TH, Channer KS. Testosterone treatment for men with chronic heart failure. Heart 2004;90:446-447.

[13] Tibenska M, Kyselovicova O, Medekova H. Anthropometric and functional changes and their relationship after two-year aerobic gymnastics training. Acta Fac Pharm Univ Comen 2010;LVII:1-7.

[14] Tibenska M, Medekova H. „Z”-scores of anthropometric and motor parameters of girls in aerobic gymnastics. Acta Fac Pharm Univ Comen 2014;LXI(2):55-58.

[15] Woodiwiss AJ, Trifunovic B, Philippides M, Norton GR. Effects of an androgenic steroid on exercise-induced cardiac remodeling in rats. J Appl Physiol 2000;88:409-415.

[16] Zammit PS, Kelly RG, Franco D, Brown N, Moorman AFM, Buckingham ME. Suppression of Atrial Myosin Gene Expression Occurs Independently in the Left and Right Ventricles of the Developing Mouse Heart. Dev Dyn 2000;217:75-85.

European Pharmaceutical Journal

Acta Facultatis Pharmaceuticae Universitatis Comenianae (formerly)

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CiteScore 2017: 0.24

SCImago Journal Rank (SJR) 2017: 0.129
Source Normalized Impact per Paper (SNIP) 2017: 0.140

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