Application of Biotests in Cyanobacterial Extract Toxicity Assessment

Open access

Abstract

The aim of the study was to determine the toxicity of the extract obtained from the cyanobacterial cells derived from the waters of Zemborzycki dam reservoir with use of a battery of biotests. The taxonomic identification of the bloom-forming cyanobacteria revealed high abundance of Aphanizomenon flos-aquae and Dolichospermum spp. (Anabaena spp.) and in a lower degree of Microcystis aeruginosa and Planktothrix agardhii. In the extract obtained from concentrated cyanobacterial cells, hepatotoxin microcystin-LR at a concentration of 22.89 ± 3.74 μg/L and neurotoxin Antx-a at 13.02 ± 0.01 μg/L have been detected. Toxicity of the extract was evaluated with the following assays: Daphtoxkit F magna with the crustacean Daphnia magna, Thamnotoxkit F with the crustacean Thamnocephalus platyurus, Rotoxkit F with the rotifer Brachionus calyciflorus and Protoxkit F with ciliate Tetrahymena thermophila. The most sensitive organism among all studied was T. platyurus for which EC50 was estimated to be 1.2% of the initial extract concentration. On the basis of the highest obtained value of the toxicity unit (TU = 83) the studied sample was classified to the IV class, which is of high acute toxicity. Additionally, it was found that reactivity on cyanobacterial products differs greatly among organisms used in bioassays, which indicate the need for using a set of biotests.

[1] Błaszczyńska, A., Toruńska, A., Kobos, J., Browarczyk-Matusiak, G. & Mazur-Marzec, H. (2010). Ecology of toxic cyanobacteria, Kosmos, 59, 173–198.

[2] James, K.J.A., Furley, I.R., Scherlock, M.A., Stack, M., Twoigh, F.B., Caudwell, O. & Skulbarg, M. (1998). Sensitive determination of anatoxin-a, homoanatoxin-a and their degradation products by liquid chromatography with fl uorometric detection, Journal of Chromatography, 789, 147–157.

[3] Lampart-Kałużniacka, M. & Śliwińska, A. (2011). Assessment of degree of eutrophization of lake Trzesiecko streams based on biotic indicators, Annual Set The Environment Protection, 13, 1069–1080.

[4] Marszalek, B. & Blaha, L. (2004). Comparison of 17 biotests for detection of cyanobacterial toxicity, Environmental Toxicology, 19, 310–317.

[5] Nicholson, B.C. & Burch, M.D. (2001). Evaluation of analytical methods for detection and quantification of cyanotoxins in relation to Australian drinking water guidelines. Cooperative Research Centre for Water Quality and Treatment, National Health and Medical Research Council of Australia, Australia. ISBN Print: 1864960949 Online: 1864961007.

[6] Pawlik-Skowrońska, B., Pirszel, J. & Kornijów, R. (2008). Spatial and temporal variation in microcystin concentrations during perennial bloom of Plantothrix agardhii in a hypertrophic lake, Annales de Limnologie, 44, 145–150.

[7] Pawlik-Skowrońska, B. & Toporowska, M. (2011). Blooms of toxin-producing Cyanobacteria – a real threat in small dam reservoirs at the beginning of their operation, Oceanological and Hydrobiological Studies, 40, 30–37.

[8] Pawlik-Skowrońska, B., Skowroński, T., Pirszel, J. & Adamczyk, A. (2004). Relationship between cyanobacterial bloom composition and anatoxin-a and microcystin occurrence in the eutrophic dam reservoir (SE Poland). Polish Journal of Ecology, 52, 479–490.

[9] Tarkowska-Kukuryk, M. (2013). Effect of phosphorous loadings on macrophytes structure and trophic state of dam reservoir on a small lowland river (Eastern Poland). Archives of Environmental Protection, 39, 3, 33–46.

[10] Persoone, G., Marsalek, B., Blinova, I., Torokne, A., Zarina, D., Manusadzianas, L., Nałęcz-Jawecki, G., Tofan, L., Stepanowa, N., Tothova, L. & Kolar, B. (2003). A practical and userfriendly toxicity classification system with microbiotests for natural waters and wastewaters, Environmental Toxicology, 18, 395–402.

[11] Sierosławska, A., Rymuszka, A., Kalinowska, R., Skowroński, T., Bownik, A., Pawlik-Skowrońska, B. (2010). Toxicity of cyanobacterial bloom in the eutrophic dam reservoir (Southeast Poland), Environmental Toxicology and Chemistry, 29, 556–560.

[12] Wiatkowski, M. & Rosik-Dulewska, Cz. (2013). Water quality assessment of Włodzienin Reservoir in the first year of its operations, Annual Set The Environment Protection (Rocznik Ochrona Środowiska), 15, 2666–2682.

[13] Wolska, L., Sagajdakow, A., Kuczyńska, A. & Namieśnik, J. (2007). Application of ecotoxicological studies in integrated environmental monitoring: Possibilities and problems, Trends in Analytical Chemistry, 26, 332–344.

[14] Sieroslawska, A. (2013). Evaluation of the sensitivity of the organisms used in commercially available toxkits to the selected cyanotoxins. Polish Journal of Environmental Studies, 22, 1817–1823.

Archives of Environmental Protection

The Journal of Institute of Environmental Engineering and Committee of Environmental Engineering of Polish Academy of Sciences

Journal Information


IMPACT FACTOR 2016: 0.708
5-year IMPACT FACTOR: 0.835

CiteScore 2017: 1.01

SCImago Journal Rank (SJR) 2017: 0.371
Source Normalized Impact per Paper (SNIP) 2017: 0.737

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 178 141 12
PDF Downloads 67 60 7