Seroprevalence Of IgG Antibodies Against Borrelia burgdorferi In Dogs In Belgrade Area, Serbia

Open access

Abstract

In this study the seroprevalence of anti-Borrelia burgdorferi IgG antibodies in populations of naturally exposed dogs in the Belgrade area was determined. Serum samples of 215 dogs were tested with in-house made indirect immunofluorescent-antibody assay (IFA), enzyme-linked immunosorbent assay (ELISA), and Western blot assay (WB). Sera were from 34 pet dogs suspected of having Lyme borreliosis, 41 hunting dogs, 125 shelter dogs, and 15 clinically healthy pet dogs. Results of WB were used as a standard against which the performances of ELISA and IFA were evaluated. ELISA was significantly more sensitive than IFA (84.8 vs 67.9%), whereas the specificities of these tests were just slightly different (94.4% vs 92.6 %). Borrelia burgdorferi seroprevalence of 24.7% among tested dogs was recorded by WB test. The highest seroprevalence of 31.2% was found in the group of shelter dogs, while hunting and pet dogs showed lower seroprevalence of 19.5% and 17.6%, but the difference was statistically insignificant. These results are the first report on the seroprevalence of IgG antibodies to B. burgdorferi in dogs in the Belgrade area and could be of importance in the assessment of the risk of infection with B. burgdorferi in human population.

1. Kurtenbach K, Peacey M, Rijpkema SG, Hoodless AN, Nuttall PA, Randolph SE: Differential transmission of the genospecies of Borrelia burgdorferi sensu lato by game birds and small rodents in England. Appl Environ Microbiol 1998, 64:1169–1174.

2. Margos G, Vollmer SA, Cornet M, Garnier M, Fingerle V, Wilske B, Bormane A, Vitorino L, Collares-Pereira M, Drancourt M, Kurtenbach K: A new Borrelia species defined by multilocus sequence analysis of housekeeping genes. Appl Environ Microbiol 2009, 75:5410-5416.

3. Lissman BA, Bosler EM, Camay H: Spirochete associated arthritis (Lyme disease] in a dog. JAVMA 1984, 185: 219–220.

4. Levy S, Dury PH: Complete heart block in a dog seropositive for Borrelia burgdorferi. J Vet Intern Med 1988, 2:138–144.

5. AzumaY, Kawamura K, Isogai H, Isogai E: Neurologic abnormalities in two dogs suspected Lyme disease. Microbiol Immunol 1993, 37:325-329.

6. Levy SA, Magnarelli LA: Relationship between development of antibodies to Borrelia burgdorferi in dogs and the subsequent development of limb/joint borreliosis. JAVMA 1992, 200:344-347.

7. Mather TN, Fish D, Coughlin RT: Competence of dogs as reservoir for Lyme disease spirochetes (Borrelia burgdorferi). JAVMA 1994, 205:186–188.

8. Eng TR, Wilson ML, Spielman A, Lastavica CC: Greater risk of Borrelia burgdorferi infection in dogs than in people. J Infect Dis 1988, 158:1410–1411.

9. Dmitrović R: Lyme disease in Yugoslavia [In Serbian: Lajmska bolest u Jugoslaviji] Velarta; Belgrade, Serbia, 1996.

10. Institute of Public Health of Serbia „Dr Milan Jovanović Batut” 2012: Health scientific yearbook Republic of Serbia [http://wwwbatutorgrs/download/publikacije/pub2012pdf].

11. Milutinović M, Radulović Ž, Jovičić V, Oreščanin Z: Population dynamics and Borrelia burgdorferi infection rate of Ixodes ricinus ticks in the Belgrade area. Acta Veterinaria (Beograd] 2004, 54:219-225.

12. Milutinović M, Masuzawa T, Tomanović S, Radulović Ž, Fukui T, Okamoto Y: Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Francisella tularensis and their co-infections in host-seeking Ixodes ricinus ticks collected in Serbia. Exp Appl Acarol 2008, 45:171–183.

13. Cekanac R, Pavlovic N, Gledovic Z, Grgurevic A, Stajkovic N, Lepsanovic Z, Ristanovic E: Prevalence of Borrelia burgdorferi in Ixodes ricinus ticks in Belgrade area. Vector Borne Zoonotic Dis 2010, 10:447-452.

14. Ristanovic ES, Kitamura K, Masuzawa T, Milutinovic MJ, Cekanac RM, Stajkovic NT, Živanovic DM: Molecular characterization of Borrelia burgdorferi sensu lato strains isolated in the area of Belgrade, Serbia. Brazilian Journal of Microbiology 2007, 38:14-16.

15. Craft JE, Grodzicki RL, Steere AC: Antibody response in Lime disease-evaluation of diagnostic tests. J Infect Dis 1984, 193:265-275.

16. Jovičić VLj, Grego EM, Lako BL, Ristović BM, Lepšanović ZA, Stajković NT: Improved serodiagnosis of early Lyme borreliosis: Immunoblot with local Borrelia afzelii strain. APMIS 2003, 311: 1053-1059.

17. Guerra MA, Walker ED, Kitron U: Quantitative Approach for the Serodiagnosis of Canine Lyme Disease by Immunoblot Procedure. J Clin Microbiol 2000, 38: 2628–2632.

18. Hauser U, Lehnert G, Lobentanzer R, Wilske B: Interpretation criteria for standardized Western blots for three European species of Borrelia burgdorferi sensu lato. J Clin Microbiol 1997, 35:1433-1444.

19. Štefančikova A, Tresova G, Petko B, Skardova I, Sesztakova E: ELISA comparison of three whole cell antigens of Borrelia burgdorferi sensu lato in serological study of dogs from area of Kosice, Eastern Slovakia. Ann Agric Environ Med 1998, 5:25–30.

20. Pejchalova, K, Žarkovska A, Fučik K, Schanilec P: Serological Confirmation of Borrelia burgdorferi Infection in Dogs in the Czech Republic. Vet Res Commun 2006, 30:231-238.

21. Greene RT, Hirsch DA, Rottman PL, Gerig TM: Interlaboratory Comparasion of Titers of Antibody to Borrelia burgdorferi and Evaluation of a Commercial Assay Using Canine Sera J Clin Microb 1991, 29:16-20.

22. Lindenmayer J, Weber M, Bryant J, Marquez E, Onderdonk A: Comparison of indirect immunofluorescent-antibody assay, enzyme-linked immunosorbent assay, and Western immunoblot for the diagnosis of Lyme disease in dogs. J Clin Microbiol 1990, 28:92–96.

23. Hovius JWR, Hovius KE, Oei A, Houwers DJ, van Dam AP: Antibodies against specific proteins of and immobilizing activity against three strains of Borrelia burgdorferi sensu lato can be found in symptomatic but not in infected asymptomatic dogs. J Clin Microbiol 2000, 38:2611-2621.

24. Savić S, Vidić B, Lazić S, Lako B, Potkonjak A, Lepsanović Z: Borrelia burgdorferi in ticks and dogs in the province of Vojvodina, Serbia. Parasite 2010, 17:357-361.

25. Leschnik M, Feiler A, Duscher GG, Joachim A: Effect of owner-controlled acaricidal treatment on tick infestation and immune response to tick-borne pathogens in naturally infested dogs from Eastern Austria. Parasit Vectors 2013, 6:62.

26. Merino FJ, Serrano JL, Saz JV, Nebreda T, Gegundez M, Beltran M: Epidemiological characteristics of dogs with Lyme borreliosis in the province of Soria (Spain]. Eur J Epidemiol 2000, 16:97–100.

27. Žarkov I, Martinov M: The Lyme disease results of a serological study in sheep, cows and dogs in Bulgaria. Revia Med Vet 2003, 154: 363-366.

28. Martinov S, Halacheva M, Nedelchev N, Aleksandrov E: Contemporary state of the tick-borne transmissible infections in domestic animals in Bulgaria. Vet Med X 2006, 3-4:7-18.

29. Potkonjak A, Savić S, Vračar V, Rnjak D, Tikvicki M, Obrenović S, Lako B: Prevalence of G class antibodies to antigens of lyme disease causes in dogs in Vojvodina, Serbia [n Serbian: Prevalencija antitela klase G na antigene uzročnika lajmske bolesti kod pasa u Vojvodini, Srbija] Vet glasnik 2013, 67:55–66.

30. Turk N, Marinculić A, Modrić Z: Serologic studies of canine Lyme borreliosis in the Zagreb area (Croatia]. Veterinarski arhiv 2000, 70:39-45.

31. Kiss T, Cadar D, Krupaci AF, Bordeanu A, Brudaşcă GF, Mihalca AD, Mircean V, Gliga L, Dumitrache MO, Spînu M: Serological reactivity to Borrelia burgdorferi sensu lato in dogs and horses from distinct areas in Romania. Vector Borne Zoonotic Dis 2011, 11:1259-1262.

32. Kybicová K, Schánilec P, Hulínská D, Uherková L, Kurzová Z, Spejchalová: Detection of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in dogs in the Czech Republic. Vector Borne Zoonotic Dis 2009, 9:655-661.

33. Barth C, Straubinger RK, Sauter-Louis C, Hartmann K: Prevalence of antibodies against Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum and their clinical relevance in dogs in Munich, Germany. Berl Munch Tierarztl Wochenschr 2012, 125:337-444.

34. Goossens HA, van den Bogaard AE, Nohlmans MK: Dogs as sentinels for human lyme borreliosis in The Netherlands. J Clin Microbiol 2001, 39:844-848.

35. Cardoso L, Mendão C, Madeira de Carvalho L: Prevalence of Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp and Leishmania infantum in apparently healthy and CVBD-suspect dogs in Portugal--a national serological study. Parasit Vectors 2012, 5:62.

36. Miró G, Montoya A, Roura X, Gálvez R, Sainz A: Seropositivity rates for agents of canine vector-borne diseases in Spain: a multicentre study. Parasit Vectors 2013, 6:117.

37. Berzina I, Matise I: Seroprevalence against Borrelia burgdorferi sensu lato and occurence of antibody co-expression with Anaplasma phagocytophilum in dogs in Latvia. Ir Vet J 2013, 66:9.

38. Volgina NS, Romashov BV, Romashova NB, Shtannikov AV: Prevalence of borreliosis, anaplasmosis, ehrlichiosis and Dirofilaria immitis in dogs and vectors in Voronezh Reserve (Russia]. Comp Immunol Microbiol Infect Dis 2013, 36:567-574.

39. Obrenović S, Radojičić S, Stević N, Bogunović D, Vakanjac S, Valčić M: Seroprevalence of cat leptospirosis in Belgrade (Serbia). Acta Veterinaria 2014, 64: 510-518.

Acta Veterinaria

The Journal of University of Belgrade

Journal Information

IMPACT FACTOR 2018: 0.656
5-year IMPACT FACTOR: 0,532

CiteScore 2018: 0.71

SCImago Journal Rank (SJR) 2018: 0.281
Source Normalized Impact per Paper (SNIP) 2018: 0.547

Cited By

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 200 171 8
PDF Downloads 69 64 5