Prevalence of Staphylococcus aureus and antibiotic resistant Staphylococcus aureus in public transport in Bratislava, Slovakia

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Abstract

There is evidence that the transmission of Staphylococcus aureus and methicillin-resistant S. aureus between the community and environmental surfaces still exists. Even the means of this transmission remain uncertain, the public transport system may serve as a potential source of different bacteria, and the contact with contaminated public surfaces may increase the risk for bacterial diseases emergence. This study aimed to investigate S. aureus contamination on Bratislava’s public transport vehicles. Forty samples of hand-touched surfaces were collected during December 2015 and March 2017 by using surface sampling method. S. aureus was detected in all analysed swabs. Simultaneously, antibiotic resistance of S. aureus from swabs was evaluated. Of 40 samples, only 23 % did not contain S. aureus resistant to some of 10 analysed antibiotics. On the other hand, the severe prevalence of highly resistant S. aureus to penicillin, methicillin, ampicillin, and cefoxitin was confirmed. 15 % of isolates displayed resistance to at least three antimicrobial classes. The amount of S. aureus was not significantly influenced by the lines or by the analysed surface (grabs rails or on-board stop buttons). However, there was a statistically significant effect of year period, both between samples from December and March and between samples from the same month but different year. The study confirmed the widespread occurrence of resistant S. aureus in public transport vehicles in Bratislava, Slovakia.

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  • Arbuthnott J Coleman D DeAzavedo J (1990) Journal of Applied Bacteriology Symposium Supplement 69: 1S—8S.

  • Bremer P Fletcher G Osborne C (2004) Staphylococcus aureus New Zealand Institute for Crop and Food Research Limited Christchurch. pp. 1—6.

  • CLSI Performance Standards for Antimicrobial Susceptibility Testing (2007) 17th Informational Supplement 27: 1—182.

  • ECDC (2017) Antimicrobial resistance surveillance in Europe 2015 Surveillance report Avialable in May 2017: http://ecdc.europa.eu/en/publications/Publications/antimicrobial-resistance-europe-2015.

  • EN ISO 6888-1 (2003) Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 1: Technique using Baird-Parker agar medium 1—11.

  • European Committee on Antimicrobial Susceptibility Testing (EUCAST 2018) Breakpoint tables for interpretation of MICs and zone diameters. Version 7.1. Available in July 2018: http//www.eucast.org.

  • Gaymard A Pichon M Degaud M Tasse J Dupieux C Laurent F (2016) International Journal of Antimicrobial Agents 48: 459—462.

  • Lowy FD (2014) Journal of Clinical Investigation 111: 1265—1273.

  • Magiorakos A-P Srinivasan A Carey RB Carmeli Y Falagas ME Giske CG et al. (2011) Clinical Microbiology and Infection 18: 268—281.

  • Mendes  Martins da Costa P Rego D Beça N Alves C Moreira T Conceiçao T Aires-de-Sousa M (2015) Public Health 129: 1125—1131.

  • Mylotte JM McDemott C Spooner JA (1987) Reviews of Infectious Diseases 9: 891—907.

  • Otter JA French GL (2009) Letters in Applied Microbiology 49: 803—805.

  • Otter JA Havill NL Boyce JM French GL (2009) Journal of Clinical Microbiology and Infectious Diseases 28: 835—839.

  • Reffuveille F Josse J Vallé Q Mongaret C Gangloff SC (2017) In Enany S Crotty Alexander LE (Eds.) The rise of virulence and antibiotic resistance in Staphylococcus aureus (pp. 189—214). InTech Zagreb.

  • Sexton J Maxwell S Gerba S (2007) 107th Annual Meeting of the American Society for Microbiology.

  • Stepanović S Ćirković I Djukić S Vuković D Švabić-Vlahović M (2008) Letters in Applied Microbiology 47: 339—341.

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