In vivo investigation of antihyperalgesic and antinociceptive effects of peat formulations

Open access

Abstract

The aim of this study is to evaluate the antihyperalgesic and antinociceptive effects of two formulations containing peat water extracts using a model of carrageenan-induced hyperalgesia, combined with a test with a mechanical stimulus, and a hot plate test. Rats were divided into seven groups (n = 6) and received local treatment with two peat formulations and two diclofenac formulations dissolved in carbopol gel and Wolff® basis creme, respectively. Carbopol gel, Wolff® basis creme and 0.9 % NaCl without tested substances were used as controls. Both peat formulations exerted an unambiguous antihyperalgesic effect 60 minutes after the treatment. In the hot plate test, the rats treated with the Wolff® basis creme peat formulation showed a tendency to prolonged latency on the first hour. The results could be explained by partial activation of peripheral α2-adrenoceptors and the possible COX-2 suppressive activity.

1. A. M. Beer, H. E. Junginger, J. Lukanov and P. Sagorchev, Evaluation of the permeation of peat substances through human skin in vitro, Int. J. Pharm. 253 (2003) 169–175; https://doi.org/10.1016/S0378-5173(02)00706-8

2. C. E. J. van Rensburg, The antiinflammatory properties of humic substances: a mini review, Phytother. Res. 29 (2015) 791–795; https://doi.org/10.1002/ptr.5319

3. R. Klocking, B. Helbig and P. Wutzler, Untersuchungen zur antiviralen Aktivität von polyanionischen Torfinhaltsstoffen in vitro und in vivo, Geburtsh. Frauenheilk. 60 (2000) 192.

4. S. Codish, M. Abdu-Shakra, D. Flusser, M. Fringer and S. Sukenik, Mud compress therapy for the hands of patients with rheumatoid arthritis, Rheumatol. Int. 25 (2005) 49–54; https://doi.org/10.1007/s00296-003-0402-4

5. A. M. Beer, J. Lukanov and P. Sagorchev, Isolation of biologically active fractions from the water soluble components of fulvic and ulmic acids from peat, Phytomedicine 9 (2002) 653–666; https://doi.org/10.1078/094471102321616490

6. J. J. Gandy, J. R. Snyman and C. E. van Rensburg, Randomized, parallel-group, double-blind, controlled study to evaluate the efficacy and safety of carbohydrate-derived fulvic acid in topical treatment of eczema, Clin. Cosmet. Investig. Dermatol. 4 (2011) 145–148; https://doi.org/10.2147/CCID.S23110

7. R. Sabi, P. Very and C. E. J. van Rensburg, Carbohydrate-derived fulvic acid (CHD-FA) inhibits carrageenan-induced inflammation and enhances wound healing: efficacy and toxicity study in rats, Drug Dev. Res. 73 (2011) 18–23; https://doi.org/10.1002/ddr.20445

8. A. M. Beer, P. Zagorchev, M. Filipova and J. Lukanov, Effects of aqueous peat extract on the activity of cyclooxygenase and cyclooxygenase-1 and cyclooxygenase-2 isoforms, Phys. Med. Rehab. Kuror. 25 (2015) 51–54.

9. S. Porzio, G. Caselli, L. Pellegrini, V. Pallottini, M. Del Rosario, A. Coppola, L. Boltri, M. Gentile, G. Clavenna and G. Melillo, Efficacy of a new topical gel-spray formulation of ketoprofen lysine salt in the rat: percutaneous permeation in vitro and in vivo and pharmacological activity, Pharmacol. Res. 37 (1998) 41–47; https://doi.org/10.1006/phrs.1997.0260

10. E. G. Apostolova, V. Y. Kokova and L. P. Peychev, Experimental study on the antinociceptive effect of retigabine in rats, IJBAMR. 5 (2015) 568–574.

11. R. M. Stepanović-Petrović, A. M. Micov, M. A. Tomić and N. D. Ugrešić, The local peripheral antihyperalgesic effect of levetiracetam and its mechanism of action in an inflammatory pain model, Anesth. Analog. 115 (2012) 1457–1466; https://doi.org/10.1213/ANE.0b013e31826c7fc2

12. Y. Honda, H. Higuchi, Y. Matsuoka, A. Yabuki-kawase, M. Ishii-maruhama, Y. Tomoyasu, S. Maeda, H. Morimatsu and T. Miyawaki, The inhibitory effect of locally injected dexmedetomidine on carrageenan-induced nociception in rats, Eur. J. Pharmacol. 764 (2015) 215–219; https://doi.org/10.1016/j.ejphar.2015.06.054

13. S. J. Chien, T. C. Chen, H. C. Kuo, C. N. Chen and S. F. Chang, Fulvic acid attenuates homocysteine-induced cyclooxygenase-2 expression in human monocytes, BMC Complement. Altern. Med. 15 (2015) 61; https://doi.org/10.1186/s12906-015-0583-xJ

14. E. Magnusson and K. Fisher, The involvement of dopamine in nociception: the role of D(1) and D(2) receptors in the dorsolateral striatum, Brain Res. 855 (2000) 260–266; https://doi.org/10.1016/S0006-8993(99)02396-3

15. L. A. Pini, G. Vitale, A. Ottani and M. Sandrini, Naloxone-reversible antinociception by paracetamol in the rat, J. Pharmacol. Exp. Ther. 280 (1997) 934–940.

Acta Pharmaceutica

The Journal of Croatian Pharmaceutical Society

Journal Information


IMPACT FACTOR 2017: 1.071
5-year IMPACT FACTOR: 1.623

CiteScore 2017: 1.46

SCImago Journal Rank (SJR) 2017: 0.362
Source Normalized Impact per Paper (SNIP) 2017: 0.642

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 66 66 36
PDF Downloads 28 28 12