Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench

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Echinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides and glycoproteins. The aim of the study was to evaluate the protein content in dried roots of Echinacea purpurea (L.) Moench after homogenization of roots with liquid nitrogen, extraction in 0.01 mol L-1 phosphate-buffered saline (PBS) and purification followed by fractionation of proteins using gel filtration chromatography. Total concentration of proteins was measured using the Bradford method, and evaluation of the molecular mass of proteins was accomplished by applying the SDS-PAGE gel electrophoresis. The Bradford assay revealed that the highest concentration of proteins in fractions collected after gel filtration chomatography was 4.66–6.07 mg mL-1. Glycoproteins, alkamides and polysaccharides in roots of Echinacea purpurea (L.) Moench are chemical compounds that are responsible for their immunomodulatory properties. However, information about the difference of protein contents in fresh and dried roots of E. purpurea is insufficient.

1. D. C. Hell, J. Barnes, L. Anderson, S. Gibbons and J. D. Phillipson, Echinacea species (Echinacea angustifolia (DC.) Hell., Echinacea pallida (Nutt.) Nutt., Echinacea purpurea (L.) Moench): a review of their chemistry, pharmacology and clinical properties, J. Pharm. Pharmacol. 57 (2005) 929-954; DOI: 10.1211/0022357056127.

2. Q. Zheng, D. Qiu, X. Liu, L. Zhang, S. Cai and X. Zhang, Antiproliferative effect of Dendrobium catenatum Lindley polypeptides against human liver, gastric and breast cancer cell lines, Food Funct. 5 (2015) 1489-1495; DOI: 10.1039/C5FO00060B.

3. J. J. Dong, F. Wu, X. Ye, C. F. Sun, Y. Y. Tian, M. X. Lu, R. Zhang and Z. H. Chen, β-Defensin in Nile tilapia (Oreochromis niloticus): Sequence, tissue expression, and anti-bacterial activity of synthetic peptides, Gene 566 (2015) 23-31; DOI: 10.1016/j.gene.2015.04.025.

4. I. Arcan and A. Yemenicioglu, Antioxidant activity of protein extracts from heat-treated or thermally processed chickpeas and white beans, Food Chem. 103 (2007) 301-312; DOI: 10.1016/j.foodchem. 2006.07.050.

5. P. Abreu, S. Matthew, T. Gonzalez, D. Costa, M. A. Segundo and E. Fernandes, Anti-inflammatory and antioxidant activity of a medicinal tincture from Pedilanthus tithymaloides, Life Sci. 78 (2006) 1578-1585; DOI: 10.1016/j.lfs.2005.07.037.

6. R. J. Simpson, Protocol: Bulk precipitation of proteins by ammonium sulfate, Cold Spring Harb. Protoc. 2006; DOI: 10.1101/pdb.prot4308.

7. A. Gupta and R. S. Sandhu, A new high molecular weight agglutinin from garlic (Allium sativum), Molec. Cell. Cell. Bioch. 166 (1997) 1-9; DOI: 10.1023/A:1006827921151.

8. H. C. Silva, A. U. Bari, B. A. Rocha, K. S. Nascimento, E. L. Ponte, A. F. Pires, P. Delatorre, E. H. Teixeira, H. Debray, A. M. Assreuy, C. S. Nagano and B. S. Cavada, Purification and primary structure of a mannose/glucose-binding lectin from Parkia biglobosa Jacq. seeds with antinociceptive and anti-inflammatory properties, J. Mol. Recogn. 26 (2013) 470-478; DOI: 10.1002/jmr.2289.

9. M. M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72 (1976) 248-254; DOI: 10.1016/0003-2697(76)90527-3.

10. K. Hyung-Keun, L. Hyuk-Min, O. Kyong-Hwa, Y. Hyo-Jin, J. Ji-Seon and K. Sook-Kyung, Interpretation of protein quantitation using the Bradford assay: Comparison with two calculation models, Anal. Biochem. 434 (2013) 178-180; DOI: 10.1016/j.ab.2012.10.045.

11. S. Thude and B. Classen, High molecular weight constituents from roots of Echinacea pallida: An arabinogalactan-protein and an arabinan, Phytochemistry 66 (2005) 1026-1032; DOI: 10.1016/j.phytochem. 2005.02.028

12. J. B. Harborne and C. A. Williams, Phytochemistry of the Genus Echinacea, in Echinacea: The genus Echinacea (Ed. S. C. Miller), CRC Press, Boca Raton 2004, pp. 61-77.

13. R. B. Volk, W. Blaschek and B. Classen, Characterization of an arabinogalactan protein from the pressed juice of Echinacea purpurea: investigations into the type of linkage between the protein and polysaccharide moieties, J. Nat. Med. 61 (2007) 397-401; DOI: 10.1007/s11418-007-0174-3.

14. A. Bossy, W. Blaschek and B. Classen, Characterization and immunolocalization of arabinogalactan- proteins in roots of Echinacea purpurea, Planta Med. 75 (2009) 1526-1533; DOI: 10.1055/ s-0029-1185801.

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